Figure 3. Increased PCR success rates and consistency with complex targets
Amplification of a 13.5kb fragment of the human ß-globin gene at different annealing temperatures (A: 68.5°C, B: 66.0°C, C: 63.0°C, D: 60.5°C). The starting template amount is 30ng human genomic DNA. GC content is 37%. Reactions were set up using master mix formats (apart from Takara’s PrimeSTAR® GXL DNA Polymerase) and following manufacturers’ recommendations. Cycling conditions were 95°C for 2 min, then 30 cycles of 95°C for 15 sec, annealing for 15 sec, 72°C for 12 min. PCRBIO HS VeriFi™ Mix displays a higher yield and specificity compared to leading competitors. The PCRBIO mix also shows greater consistency and versatility across the annealing temperature range.