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PCRBIO HS VeriFi™ Polymerase

제품 소개

PCRBIO HS VeriFi™ Polymerase는 정밀한 PCR을 수행 가능하도록 AptaLock™ hot start technology가 적용된 강력한 proofreading enzyme입니다.
향상된 DNA 결합 능력과 처리 능력으로 speed, yield and sensitivity 를 크게 개선하는 동시에 길고 까다로운 템플릿의 PCR 성공률을 크게 높여줍니다.

AptaLock™ is an innovative proprietary technology that uses an aptamer-like molecule to confer hot start activity to the polymerase. It is an alternative method to an antibody-mediated hot start.  The AptaLock™ molecule reversibly binds and inhibits the exonuclease and polymerase activities of the enzyme at temperatures below 40°C. This allows the reaction to be set up at room temperature without the loss of sensitivity and specificity as a result of primer dimer formation and non-specific amplification.

제품특징

• sensitivity and specificity 를 극대화하는 AptaLock™ hot start technology
• 길거나 GC/AT 가 많은 템플릿(>17.5kb)도 쉽게 진행
• 최대 100°C에서 denaturation이 가능하므로 GC-rich sequences도 보다 효과적으로 분리 가능
• Taq DNA 중합효소보다 100배 높은 충실도
• 실온에서 셋업 가능
• Reaction mix 는 PCR 실행 전/후 모두 최대 24시간 동안 안정적.
• blunt-end PCR products 생성

응용분야

• High fidelity PCR
• Long PCR
• Multiplex and high throughput PCR
• Site-directed mutagenesis
• Cloning
• Sequencing

보관온도

• -20℃

Figure 1. Superior performance in multiplex reactions
10-plex PCR using lambda phage genome (6 targets) and mouse genome (4 targets) at different annealing temperatures (A: 63.0°C, B: 61.5°C, C: 60.5°C). The starting template amount is 1pg lambda DNA and 1ng mouse gDNA. Amplicon lengths are between 139bp and 962bp. Reactions were set up using master mix formats following manufacturers’ recommendations. Cycling conditions were 95°C 2 min, 40 cycles of 95°C 15 sec, annealing A to C 30 sec, 72°C 90 sec. L:
PCRBIO Ladder III. P: reference pool of single products. PCRBIO HS VeriFi™ Mix displays greater sensitivity and specificity in multiplex when compared to leading competitors

Figure 2. Successful PCR across a broad range of GC and AT content
Amplification of 13 targets with GC content ranging from 28.7% to 83% using PCRBIO HS VeriFi™ Mix. The starting template amount is 30ng mouse cDNA. Band size is between 99bp and 274bp. Cycling conditions were 98°C 5 min, 40 cycles of 98°C 15 sec, annealing between 54°C and 62°C (depending on target) 15 sec, 72°C 30 sec. L: PCRBIO Ladder III. PCRBIO HS VeriFi™ Mix is able to amplify templates across a broad range of GC and AT content.

Figure 3. Increased PCR success rates and consistency with complex targets
Amplification of a 13.5kb fragment of the human ß-globin gene at different annealing temperatures (A: 68.5°C, B: 66.0°C, C: 63.0°C, D: 60.5°C). The starting template amount is 30ng human genomic DNA. GC content is 37%. Reactions were set up using master mix formats (apart from Takara’s PrimeSTAR® GXL DNA Polymerase) and following manufacturers’ recommendations. Cycling conditions were 95°C for 2 min, then 30 cycles of 95°C for 15 sec, annealing for 15 sec, 72°C for 12 min. PCRBIO HS VeriFi™ Mix displays a higher yield and specificity compared to leading competitors. The PCRBIO mix also shows greater consistency and versatility across the annealing temperature range.

주문정보

Cat. No. 품명 규격 제조사 비고
PB10.45-01 PCRBIO HS VeriFi Polymerase 100 Units PCRbiosystems [1 x 0.05mL 2u/µL] & [1 x 1.7mL buffer
PB10.45-05 PCRBIO HS VeriFi Polymerase 500 Units PCRbiosystems [1 x 0.250mL 2u/µL] & [3 x 1.7mL buffer]
PB10.46-01 PCRBIO HS VeriFi Mix 100 x 50µL Reactions PCRbiosystems 2 x 1.25mL
PB10.45-05 PCRBIO HS VeriFi Mix 500 x 50µL Reactions PCRbiosystems 2 x (5 x 1.25mL)
PB10.47-01  PCRBIO HS VeriFi Mix Red 100 x 50µL Reactions PCRbiosystems 2 x 1.25mL
PB10.47-05  PCRBIO HS VeriFi Mix Red 500 x 50µL Reactions PCRbiosystems 2 x (5 x 1.25mL)

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