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PCRBIO Rapid Extract PCR Kit

제품 소개

Direct PCR이란?
준비된 시료로부터 별도의 DNA 추출 과정 없이 시료 자체를 바로 PCR에 사용하는 방법입니다.
시료에서 PCR 반응 단계까지의 준비 시간이 짧고 실험 방법이 간단하며 다수의 시료를 빠른 시간 내에 PCR 할 수 있다는 장점이 있습니다.


PCRBIO Rapid Extract Lysis Kit는 다양한 샘플로부터 DNA 추출과정 없이 15분 이내에 바로 PCR을 수행 할 수 있습니다.
Rapid Extraction PCR kit는 PCRBIO HS Taq Mix Red가 포함되어 있는 제품으로 Non specific band나 primer dimer 등 실험과정 중에 발생할 수 있는 문제를 줄이고 편리성을 높였습니다.

제품특징

• 신속, 편리한 single-tube DNA extraction kit
• high yield, 15분 이내에 DNA 추출 가능
• PCRBIO HS Taq Mix Red 를 통한 direct gel loading
• Sample: Mouse tail clip and ear punch / Animal tissue / Hair follicle / Buccal swab / Mammalian blood / FFPE tissue

응용분야

• Genotyping
• Transgene Detection
• Knockout Analysis
• Sequencing

보관온도

•-20℃

Figure 1. Mouse tail DNA rapid extraction.
PCRBIO Rapid Extract PCR Kit was used to extract DNA from 3mg of mouse tail clipping following the standard 15-minute protocol. Row 1 shows results from PCR Biosystems, The line below is the result of the competitor’s products.

Figure 2. Mouse ear DNA rapid extraction and 2.5kb amplification
1) Add sample directly to lysis buffer
2) 5-10 minute incubation
3) 10-minute inactivation and pellet debris by centrifugation
4) Add to PCRBIO HS Taq Mix Red and amplify

주문정보

​ 제품번호 품명 규격 제조사 비고
PB10.24-08 PCRBIO Rapid Extract PCR Kit 80 rxns PCRbiosystems [PCRBIO HS Taq Mix Red 2 x 1ml] & [Buffer A 1 x 1.6ml, Buffer B 1 x 0.8ml]
PB10.24-40 PCRBIO Rapid Extract PCR Kit 400 rxns PCRbiosystems [PCRBIO HS Taq Mix Red 10 x 1ml] & [Buffer A 5 x 1.6ml, Buffer B 5 x 0.8ml]
PB15.11-08 PCRBIO Rapid Extract Lysis kit 80 rxns PCRbiosystems Buffer A 1 x 1.6ml, Buffer B 1 x 0.8ml
PB15.11-24 PCRBIO Rapid Extract Lysis kit 240 rxns PCRbiosystems Buffer A 3 x 1.6ml, Buffer B 3 x 0.8ml

관련 논문

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