Figure 3. High consistency and reproducibility of quantification. Quantification of 16 adapter-ligated libraries using NGSBIO Library Quant Kit (purple), NEBNext® Library Quant Kit (orange), and K APA Library Quantification Kit (green). The NGSBIO Library Quant Kit shows less spread and greater consistency among replicates. The quantification results are within those obtained by two leading manufacturers of NGS library quantification kits. The number on the bottom right corner of each graph represents the concentration of dsDNA obtained using a Qubit Fluorometer from Invitrogen.