NGSBIO Library Quant Kit for Illumina

Figure 1. Amplification Curves. An adapter-ligated library sample (purple) is run alongside six standard templates (grey) provided in the NGSBIO Library Quant Kit.
Figure 2. Standard Curve. The Cts of the amplification curves is plotted against the log of the concentration of the standard templates. A linear curve is fitted through the standards. The concentration of the unknown sample is then calculated from its position on the curve.

Figure 3. High consistency and reproducibility of quantification. Quantification of 16 adapter-ligated libraries using NGSBIO Library Quant Kit (purple), NEBNext® Library Quant Kit (orange), and K APA Library Quantification Kit (green). The NGSBIO Library Quant Kit shows less spread and greater consistency among replicates. The quantification results are within those obtained by two leading manufacturers of NGS library quantification kits. The number on the bottom right corner of each graph represents the concentration of dsDNA obtained using a Qubit Fluorometer from Invitrogen.