Project Description

qPCRBIO Genotyping Mix

제품 소개

qPCRBIO Genotyping Mix는 TaqMan probe, Molecular Beacon probe등을 이용하여 유전형질 (genotyping) 분석을 할 수 있습니다.
이 제품은 hot start polymerase, dNTPs, buffer, MgCl2가 혼합 된 형태로 target gene에 대한 매우 높은 특이도를 갖고 있어 목적 DNA의 증폭에 매우 효과적dlq니다.
또한 이 제품의 buffer 조성은 allele specific probe가 더욱 특이적으로 binding 할 수 있도록 하며 clear한 allele cluster를 만들 수 있습니다.

제품특징

• 정확한 대립 유전자 식별
• 우수한 allele clustering
• TaqMan™ dual-labeled probe와 호환

응용분야

• Genotyping single nucleotide polymorphisms (SNPs)
• TaqMan® allelic discrimination assays
• High throughput genotyping studies
• TaqMan® pre-designed SNP genotyping assays

보관온도

•-20℃

HRM analysis of class I, II, III and IV single nucleotide polymorphisms. In each case, three samples of human genomic DNA were amplified using qPCRBIO HRM Mix with primers specific for a fragment of gene containing the SNPs. Figure 1 shows Factor V 1691G/A (class I), figure 2 shows MTHFR 1298A/C (class II) After amplification the products were subjected to HRM analysis. The traces show clear allele calling of class I, II, III and IV SNPs.

HRM analysis of class I, II, III and IV single nucleotide polymorphisms. In each case, three samples of human genomic DNA were amplified using qPCRBIO HRM Mix with primers specific for a fragment of gene containing the SNPs. figure 3 shows HFE 187 C/G (class III) and figure 4 shows beta-globulin 17A/T (class IV). After amplification the products were subjected to HRM analysis. The traces show clear allele calling of class I, II, III and IV SNPs.

주문정보

​ 제품번호 품명 규격 제조사 비고
PB20.41-01 qPCRBIO Genotyping Mix Lo-ROX 1 x 1mL PCRBIO Systems
PB20.41-05 5 x 1mL
PB20.42-01 qPCRBIO Genotyping Mix Hi-ROX 1 x 1mL
PB20.42-05 5 x 1mL
PB20.43-01 qPCRBIO Genotyping Mix No-ROX 1 x 1mL
PB20.43-05 5 x 1mL

관련 논문

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Document

제품 자료 Genotyping Mix Product Flyer.pdf Download Size : 680kB
제품 MSDS PB20 Real-Time Mixes MSDS.pdf Download Size : 324kB
제품 매뉴얼 Genotyping Mix Lo-ROX Manual.pdf Download Size : 57.6kB