Project Description
Claro Supremo ECL solution
제품 소개
Claro ECL solution은 웨스턴 블랏 (Western blot)을 위한 detection reagent로, 낮은 antibody 농도에서도 높은 감광을 보입니다.
Solution A (Luminol/enhancer)와 Solution B (Peroxide)를 각각 1:1의 비율로 혼합하여 사용합니다.
민감도에 따라4가지 제품군으로 나뉘어 있어, 사용자의 목적에 맞게 선택이 가능합니다.
Claro Supremo ECL solution은 mid-femtogram 에서 low-femtogram 검출 레벨에 안정적인 광 출력을 갖는 ECL substrate입니다.
이 제품은 매우 높은 signal intensity와 signal을 장시간 유지해야 하는 application을 위한 분들에게 적합한 제품입니다.
제품특징
• 고감도
• Quantitative: linear dynamic range
• 긴 신호 지속 시간
• Solution A: Luminol/enhancer solution (amber bottle) / Solution B: Peroxide solution (white bottle)
• Mid-femtogram detection
• 3일 동안 안정한 작업 용액
• background 대비 뚜렷한 signal 확인 가능
응용분야
• Routine protein detection
• Protein confirmation
• Overexpressed proteins
• Tagged proteins
보관온도
• Storage at room temperature 1 year
Figure1. Hela whole cell lysate (Abcam ® ) was diluted to 250 μg/mL in electrophoresis reducing sample buffer and 1:1 serial dilutions were made. 5μL of each dilution (1250 ng to 78 ng of total protein) were separated by SDS-PAGE and the proteins were transferred to Amersham™ Protran ® Premium 0,45 NC membrane (GEHC). The membrane was blocked with 5% non-fat dry milk in 1X TBS TWEEN ® 20 Buffer. Then the membrane was incubated with Rabbit Anti-MAP Kinase (ERK 1/2) antibody (SIGMA) at 1:5000 followed by incubation with Anti-Rabbit IgG HRP Linked F(ab′)2 (GEHC) at 1:50000.
주문정보
| Cat. No. | 품명 | 규격 | 제조사 | Antibody Dilution Range | Competitor |
| A0030 (HQS071, 0250) |
Claro Sola ECL Solution -Low picogram |
2 x 125mL | BioD | Primary: 1:500 – 1:5,000 Secondary: 1:20,000 – 1:100,000 |
Pierce™ ECL plus – Thermo scientific™ Immobilon® classico – Millipore™ Western lightning™ plus – PerkinElmer Westernbright™ ecl – Advansta |
| A0031 (HQS070, 0250) |
Claro Mucho ECL Solution -Mid femtogram |
2 x 125mL | BioD | Primary: 1:1,000 – 1:15,000 Secondary: 1:25,000 – 1:150,000 |
Clarity™ – Bio-rad Supersignal™ west dura – Thermo scientific™ Amersham™ ecl™ prime – GE healthcare Supersignal™ west pico plus – Thermo scientific™ Immobilon® crescendo – Millipore™ Westernbright™ quantum™ – Advansta |
| A0032 (HQS075, 0100) |
Claro Supremo ECL Solution -Mid femtogram to low femtogram |
2 x 50mL | BioD | Primary: 1:5,000 – 1:50,000 Secondary: 1:50,000 – 1:250,000 |
Supersignal™ west dura – Thermo scientific™ Amersham™ ECL prime™ – GE healthcare Immobilon® forte – Millipore™ Immobilon® – Millipore™ Western lightning™pro – PerkinElmer |
| A0033 (HQS3,0100) |
Claro SupremoPlus ECL Solution -Low femtogram |
2 x 50mL | BioD | Primary: 1:5.000 – 1:100.000 Secondary: 1:100.000 – 1:500.000 |
Clarity max™ – Bio-rad Supersignal™ west femto – Thermo scientific™ Amersham™ ECL select™ – GE healthcare Westernbright™ sirius™ – Advansta Western lightning™ultra – PerkinElmer |
Document
| 제품 자료 | ECL Selection guide_web.pdf | Upload date: 2021.07.14 |
| 제품 매뉴얼 | Manual-Supremo-ECL.pdf | Upload date: 2021.07.14 |
| 제품 MSDS | MSDS-Supremo-Luminol.pdf MSDS-Supremo-peroxide.pdf |
Upload date: 2021.07.14 |