Project Description

Claro™ Supremo ECL solution

제품 소개

Claro ECL solution은 Western blot을 위한 detection reagent로, 낮은 antibody 농도에서도 높은 감광을 보입니다.
Solution A (Luminol/enhancer)와 Solution B (Peroxide)를 각각 1:1의 비율로 혼합하여 사용합니다.
민감도에 따라 3가지 제품군으로 나뉘어 있어, 사용자의 목적에 맞게 선택이 가능합니다.

Claro Supremo ECL solution은 mid-femtogram 에서 low-femtogram 검출 레벨에 안정적인 광 출력을 갖는 ECL substrate입니다.
이 제품은 매우 높은 signal intensity와 signal을 장시간 유지해야 하는 application을 위한 분들에게 적합한 제품입니다.

제품특징

• 고감도
• Quantitative: linear dynamic range
• 긴 신호 지속 시간
• Solution A: Luminol/enhancer solution (amber bottle) / Solution B: Peroxide solution (white bottle)
• Mid-femtogram detection
• 3일 동안 안정한 작업 용액
• background 대비 뚜렷한 signal 확인 가능

응용분야

• Routine protein detection
• Protein confirmation
• Overexpressed proteins
• Tagged proteins

보관온도

• Storage at room temperature 1 year

Figure1. Hela whole cell lysate (Abcam ® ) was diluted to 250 μg/mL in electrophoresis reducing sample buffer and  1:1  serial dilutions were made.  5μL  of each dilution (1250 ng to 78 ng of total protein) were separated by SDS-PAGE  and the proteins were transferred to  Amersham™  Protran ®   Premium  0,45  NC  membrane (GEHC). The membrane was blocked with 5% non-fat dry milk in  1X  TBS  TWEEN ® 20  Buffer.  Then the membrane was incubated with  Rabbit Anti-MAP Kinase (ERK 1/2) antibody (SIGMA) at 1:5000 followed by incubation with Anti-Rabbit IgG HRP Linked F(ab′)2 (GEHC) at 1:50000.

주문정보

Cat. No. 품명 규격 제조사 Antibody Dilution Range Competitor
HQS071, 0250 Claro Sola ECL Solution
-Low picogram
2 x 125mL BioD Primary:
1:500 – 1:5,000
Secondary:
1:20,000 – 1:100,000
Pierce™ ECL plus – Thermo scientific™
Immobilon® classico – Millipore™
Western lightning™ plus – PerkinElmer
Westernbright™ ecl – Advansta
HQS070, 0250 Claro Mucho ECL Solution
-Mid femtogram
2 x 125mL BioD Primary:
1:1,000 – 1:15,000
Secondary:
1:25,000 – 1:150,000
Clarity™ – Bio-rad
Supersignal™ west dura – Thermo scientific™
Amersham™ ecl™ prime – GE healthcare
Supersignal™ west pico plus – Thermo scientific™
Immobilon® crescendo – Millipore™
Westernbright™ quantum™ – Advansta
HQS075, 0100 Claro Supremo ECL Solution
-Mid femtogram to  low femtogram
2 x 50mL BioD Primary:
1:5,000 – 1:50,000
Secondary:
1:50,000 – 1:250,000
Supersignal™ west dura – Thermo scientific™
Amersham™ ECL prime™ – GE healthcare
Immobilon® forte – Millipore™
Immobilon® – Millipore™
Western lightning™pro – PerkinElmer
HQS3,0100 Claro SupremoPlus ECL Solution
-Low femtogram
2 x 50mL BioD Primary:
1:5.000 – 1:100.000
Secondary:
1:100.000 – 1:500.000
Clarity max™ – Bio-rad
Supersignal™ west femto – Thermo scientific™
Amersham™ ECL select™ – GE healthcare
Westernbright™ sirius™ – Advansta
Western lightning™ultra – PerkinElmer

Document

제품 자료 BioD-Claro-ECL-solution.pdf
ECL Selection guide_web.pdf
Upload date: 2021.07.14
제품 매뉴얼 Manual-Supremo-ECL.pdf Upload date: 2021.07.14
제품 MSDS MSDS-Supremo-Luminol.pdf
MSDS-Supremo-peroxide.pdf
Upload date: 2021.07.14