Introduction

Metabion DLP standard portfolio에는 minor groove binder (MGB) probes 도 포함되어 있습니다.
아래 다양한 5’ dye / 3’ NFQ-MGB combinations을 확인하고 문의 주시면 담당자가 즉시 연락 드리겠습니다.
커스텀 조합이나 공장 단위 대량 합성도 가능합니다.

MGB in comparison to ZNA®-conjugated, LNA-modified oligonucleotides

MGB (Minor Groove Binder), ZNA®, LNA(Locked Nucleic Acids), and PNA (Peptide Nucleic Acid) are known to increase the Tm of an oligo sequence.
MGB (Minor Groove Binder) probes include a minor groove binder (MGB) moiety at the 3’ end that increases the melting temperature (Tm) of the probe and stabilizes the hybridization of the probe DNA to its target sequence.
The introduction of the minor groove binder moiety is sequence-independent, meaning the core sequence remains “unmodified”.

ZNA®s are oligonucleotides conjugated with repeated cationic spermine units that decrease electrostatic repulsions with target nucleic acid strands, and greatly improve hybridization properties by enhanced affinity to the complementary target sequence as well as increased stability of the formed duplex at an unprecedented specificity.
The “Tm boost” generated by adding ZNA® to either end of the oligonucleotide probe is significant and also sequence-independent.

In contrast, LNA probes rely on modified nucleotide chemistry with regard to the sugar component involved, while the organic base component is “unmodified” and thus follows Watson-Crick base-pairing rules when mixed with DNA or RNA bases in an oligonucleotide.
When incorporated into an oligonucleotide probe, locked nucleic acid monomers increase structural stability, resulting in a raise of the formed duplex melting temperature (Tm).
Locked Nucleic acids are not recognized by DNA/RNases as a substrate, hence LNA modified oligonucleotides also display significant resistance to nucleases.

In summary, Metabion offers all three duplex stability-enhancing modifications, and therefore provides the greatest flexibility in assay design and choosing the right option for your required application.

Delivery (발송)

Metabion normal olgo는 working days 기준 7~10일 정도 소요 되니다.
Modified oligo는 여기에 3-5일  이상 추가 시간이 소요 됩니다.

FAQ

What kind of product documentation do I get with my MGB probes?
A hard copy of the HPLC chromatogram and the mass spectrum are included in addition to the respective Synthesis Report and delivery note.

What concentration of MGB probes are recommended for PCR applications?
A concentration of 0.2 µM of the MGB probe should be fine for most assays, but as usual, the optimal concentration should be determined experimentally.
Due to the higher affinity of the probe to the target, the probe concentration might be lower.

Oligo 보관시 유의점

일반적으로 올리고는 화학적으로 안정하며, -20℃에서 6개월 이상 안정하다고 볼 수 있습니다.
특히, 동결 건조한 상태에서는 매우 안정적이며 오래 보관하실 수 있습니다.
용액 상태에서도 안정하지만, nuclease등의 contamination에 의해 degradation 될 수도 있습니다.

또한, 올리고는 얼렸다 녹였다’를 반복하게 되면 분해를 촉진시켜 품질의 이상이 발생될 수 있습니다.
DNA의 경우는 acidic한 조건에서는 depurination에 의해 decompose될 수 있으니 pH를 낮추지 말아야 합니다.

DNA Dual Labelled Probes (included MGB probe) 주문하는 방법

아래 링크의 올리고 주문양식을 다운로드하여 각 항목을 빠짐없이 작성후 oligo@bio-d.co.kr 로 보내주시면 됩니다.