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GenCRISPR™ gRNA/Cas9 Plasmids

GenScript는 사전 검증된 가이드 RNA(gRNA) 서열을 포함하는 다양한 종류의 CRISPR/Cas9 Plasmid 시스템을 제공합니다.
Plasmid는 gRNA 데이터베이스에서 유전자 이름, 기호 또는 ID로 검색할 수 있습니다.

진스크립트 CRISPR/Cas9 서비스 특징

• 온라인 Tool에서 쉽게 원하는 유전자의 sgRNA를 선택하거나 design할 수 있음
• GenScript 만의 유전자 합성 기술로 ssDNA/dsDNA 모두 전문적으로 합성 가능
• 다양한 Vector를 저렴한 가격에 제공하여 초기 시작 부담이 적음
• End to end solution: sgRNA design tool, 유전자 합성, 다양한 CRISPR/Cas9 방법 등 실험에 필요한 모든 서비스 제공

GenScript CRISPR Plasmid Repository

GenScript maintains a collection of more than 20,000 lentiCRISPRv2 plasmids containing guide RNA (gRNA) sequences pre-validated by the Broad Institute. Plasmids can be searched by gene name, symbol, or ID on our gRNA Database.

Product Vector Selection
GenCRISPR™ Plasmid Collection Lentiviral Amp, Puro

Enhanced CRISPR/SpCas9 Plasmids

Enhanced specificity SpCas9 (eSpCas9), also referred to as SpCas9 (K848A/K1003A/R1060A), is structurally engineered for improved target specificity by researchers at the Feng Zhang Laboratory at the Broad Institute (Slaymaker et al. 2016).  eSpCas9 is able to reduce off-target effects by over 10-fold while maintaining robust on-target genome editing efficiency.

Product Vector Selection
eSpCas9 Plasmids Plasmid
Lentiviral
Puro or GFP

SpCas9 Plasmids

Cas9 endonuclease is the research standard for gene editing. When combined with single guide RNA (sgRNA) sequences, these enzymes create site-specific double-strand breaks (DSBs) in the genome.

Product Vector Selection
SpCas9 Plasmids Plasmid
Lentiviral
AAV
Amp
Amp, Puro
Amp, Neo
Amp, GFP

SpCas9 Nickase Plasmids

SpCas9 nickase (Cas9n D10A) contains a mutation allowing the endonuclease to create single-strand nicks, as opposed to DSBs. Pairing two opposite facing gRNA sequences with SpCas9 nickase is an efficient method of gene editing that prevents unwanted indels from forming.

Product Vector Selection
SpCas9 Nickase Plasmids Plasmid
Lentiviral
Amp
Amp, Puro
Amp, GFP

SaCas9 Plasmids

The Staphylococcus aureus Cas9 orthologue (SaCas9) is the preferred endonuclease for adeno-associated virus (AAV) applications. SaCas9 is approximately 1 kb shorter than SpCas9, and offers additional flexibility around AAV packaging constraints. The lower immunogenicity of AAV vectors makes SaCas9 well-suited for in vivo editing applications and therapeutics.

Product Vector Selection
SaCas9 Plasmids AVV Amp

Transcription Activation (SAM) Plasmids

The CRISPR/Cas9 Synergistic Activation Mediator (SAM) system has been engineered to enable transcriptional activation of downstream targets. The SAM system utilizes three different activators, VP64, P65, and HSF1, which are assembled onto a catalytically dead Cas9 (dCas9) complex to drive transcription.

Product Vector Selection
SAM gRNA Plasmids Plasmid
Lentiviral
Amp
Amp, Zeo
SAM dCas9-VP64 Plasmids Lentiviral Amp, Blast
Amp, GFP
SAM MS2-P65-HSF1 Plasmids Lentiviral Amp, GFP
Amp, Hygro

Broad Institute Plasmid Collection

Broad Institute Plasmids are generated by the Broad Institute of Harvard and MIT. These plasmids contain a 17bp-1.8kb expressible linker in lieu of a customized sgRNA sequence, which can be modified by your laboratory.

Product Vector Selection
eSpCas9 and SpCas9 Broad Plasmid Collection Plasmid
Lentiviral
Amp, Puro
Amp, GFP
SpCas9 Nickase
Broad Plasmid Collection
Plasmid Amp
Amp, Puro
Amp, GFP
SaCas9
Broad Plasmid Collection
AAV Amp

 

CRISPR Plasmid Detail

Service Vector Cas9/gRNA Expression Delivery Method Vector Type Selection Marker
eSpCas9 Plasmids eSpCas9-2A-GFP (PX458 eSpCas9 & gRNA Plasmid All-in-one Vector AmpR
EGFP
eSpCas9 Plasmids eSpCas9-2A-Puro (PX459) V2. eSpCas9 & gRNA Plasmid All-in-one Vector AmpR
PuroR
eSpCas9 Plasmids eSpCas9-LentiCRISPR v eSpCas9 & gRNA Lentiviral All-in-one Vector AmpR
PuroR
SpCas9 Plasmids pSpCas9 BB-2A-GFP PX458 SpCas9 & gRNA Plasmid All-in-one Vector AmpR
EGFP
SpCas9 Plasmids pSpCas9 BB-2A-Puro (PX459) v2.0 SpCas9 & gRNA Plasmid All-in-one Vector AmpR
PuroR
SpCas9 Plasmids pLentiCRISPR v2 SpCas9 & gRNA Lentiviral All-in-one Vector AmpR
PuroR
SpCas9 Plasmids
SpCas9 Nickase Plasmids
pLentiGuide-Puro gRNA Only Lentiviral Dual Vector AmpR
PuroR
SpCas9 Plasmids pLentiCas9-Blast SpCas9 Only Lentiviral Dual Vector AmpR
BsdR
BleoR
SpCas9 Plasmids pLentiCas9-EGFP SpCas9 Only Lentiviral Dual Vector AmpR
EGFP
SpCas9 Plasmids pGS-gRNA gRNA Only Plasmid Dual Vector AmpR
SpCas9 Plasmids pGS-gRNA-Neo gRNA Only Plasmid Dual Vector AmpR
NeoR
SpCas9 Plasmids pSpCas9 PX165 SpCas9 Only Plasmid Dual Vector AmpR
SpCas9 Plasmids pAAV_SpGuide acceptor (PX552) gRNA Only AAV Dual Vector AmpR
EGFP
SpCas9 Plasmids pAAV-SpCas9 PX551 SpCas9 Only AAV Dual Vector AmpR
SpCas9 Nickase Plasmids pSpCas9n BB PX460 SpCas9 Nickase & gRNA Plasmid All-in-one Vector AmpR
SpCas9 Nickase Plasmids pSpCas9n BB-2A-GFP PX461 SpCas9 Nickase & gRNA Plasmid All-in-one Vector AmpR
EGFP
SpCas9 Nickase Plasmids pSpCas9n BB-2A-Puro (PX462) V2.0 SpCas9 Nickase & gRNA Plasmid All-in-one Vector AmpR
PuroR
SpCas9 Nickase Plasmids pLentiCas9n-Blast SpCas9 Nickase Only Lentiviral Dual Vector AmpR
BsdR
BleoR
SaCas9 Plasmids pX601_AAV SaCas9 & gRNA AAV All-in-one Vector AmpR
Transcription Activation (SAM) pSgRNA(MS2) gRNA Only Plasmid SAM Multi Vector AmpR
Transcription Activation (SAM) pLenti_sgRNA(MS2)_zeo gRNA Only Lentiviral SAM Multi Vector AmpR
ZeoR
BeloR
Transcription Activation (SAM) pLenti_dCas9-VP64_Blast Cas9 Activator Lentiviral SAM Multi Vector AmpR
BlastR
BleoR
Transcription Activation (SAM) pLenti_dCas9-VP64_GFP Cas9 Activator Lentiviral SAM Multi Vector AmpR
EGFP
BleoR
Transcription Activation (SAM) pLenti_MS2-P65-HSF1_Hygro Activator Adapter Lentiviral Multi Vector AmpR
HygroR
BleoR
Transcription Activation (SAM) pLenti_MS2-P65-HSF1_GFP Activator Adapter Lentiviral Multi Vector AmpR
EGFP
BleoR

CRISPR/Cas9 gRNA Vector Design Guide

CRISPR/Cas9 gRNA Vector