GenScript CRISPR Plasmid Repository

GenScript maintains a collection of more than 20,000 lentiCRISPRv2 plasmids containing guide RNA (gRNA) sequences pre-validated by the Broad Institute. Plasmids can be searched by gene name, symbol, or ID on our gRNA Database.

Enhanced CRISPR/SpCas9 Plasmids

Enhanced specificity SpCas9 (eSpCas9), also referred to as SpCas9 (K848A/K1003A/R1060A), is structurally engineered for improved target specificity by researchers at the Feng Zhang Laboratory at the Broad Institute (Slaymaker et al. 2016).  eSpCas9 is able to reduce off-target effects by over 10-fold while maintaining robust on-target genome editing efficiency.
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SpCas9 Plasmids

Cas9 endonuclease is the research standard for gene editing. When combined with single guide RNA (sgRNA) sequences, these enzymes create site-specific double-strand breaks (DSBs) in the genome.
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SpCas9 Nickase Plasmids

SpCas9 nickase (Cas9n D10A) contains a mutation allowing the endonuclease to create single-strand nicks, as opposed to DSBs. Pairing two opposite facing gRNA sequences with SpCas9 nickase is an efficient method of gene editing that prevents unwanted indels from forming.

SaCas9 Plasmids

The Staphylococcus aureus Cas9 orthologue (SaCas9) is the preferred endonuclease for adeno-associated virus (AAV) applications. SaCas9 is approximately 1 kb shorter than SpCas9, and offers additional flexibility around AAV packaging constraints. The lower immunogenicity of AAV vectors makes SaCas9 well-suited for in vivo editing applications and therapeutics.
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Transcription Activation (SAM) Plasmids

The CRISPR/Cas9 Synergistic Activation Mediator (SAM) system has been engineered to enable transcriptional activation of downstream targets. The SAM system utilizes three different activators, VP64, P65, and HSF1, which are assembled onto a catalytically dead Cas9 (dCas9) complex to drive transcription.
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Broad Institute Plasmid Collection

Broad Institute Plasmids are generated by the Broad Institute of Harvard and MIT. These plasmids contain a 17bp-1.8kb expressible linker in lieu of a customized sgRNA sequence, which can be modified by your laboratory.