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GenCRISPR™ gRNA/Cas9 Plasmids
GenScript는 사전 검증된 가이드 RNA(gRNA) 서열을 포함하는 다양한 종류의 CRISPR/Cas9 Plasmid 시스템을 제공합니다.
Plasmid는 gRNA 데이터베이스에서 유전자 이름, 기호 또는 ID로 검색할 수 있습니다.
진스크립트 CRISPR/Cas9 서비스 특징
• 온라인 Tool에서 쉽게 원하는 유전자의 sgRNA를 선택하거나 design할 수 있음
• GenScript 만의 유전자 합성 기술로 ssDNA/dsDNA 모두 전문적으로 합성 가능
• 다양한 Vector를 저렴한 가격에 제공하여 초기 시작 부담이 적음
• End to end solution: sgRNA design tool, 유전자 합성, 다양한 CRISPR/Cas9 방법 등 실험에 필요한 모든 서비스 제공
GenScript CRISPR Plasmid Repository
GenScript maintains a collection of more than 20,000 lentiCRISPRv2 plasmids containing guide RNA (gRNA) sequences pre-validated by the Broad Institute. Plasmids can be searched by gene name, symbol, or ID on our gRNA Database.
Product | Vector | Selection |
GenCRISPR™ Plasmid Collection | Lentiviral | Amp, Puro |
Enhanced CRISPR/SpCas9 Plasmids
Enhanced specificity SpCas9 (eSpCas9), also referred to as SpCas9 (K848A/K1003A/R1060A), is structurally engineered for improved target specificity by researchers at the Feng Zhang Laboratory at the Broad Institute (Slaymaker et al. 2016). eSpCas9 is able to reduce off-target effects by over 10-fold while maintaining robust on-target genome editing efficiency.
More information »
Product | Vector | Selection |
eSpCas9 Plasmids | Plasmid Lentiviral |
Puro or GFP |
SpCas9 Plasmids
Cas9 endonuclease is the research standard for gene editing. When combined with single guide RNA (sgRNA) sequences, these enzymes create site-specific double-strand breaks (DSBs) in the genome.
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Product | Vector | Selection |
SpCas9 Plasmids | Plasmid Lentiviral AAV |
Amp Amp, Puro Amp, Neo Amp, GFP |
SpCas9 Nickase Plasmids
SpCas9 nickase (Cas9n D10A) contains a mutation allowing the endonuclease to create single-strand nicks, as opposed to DSBs. Pairing two opposite facing gRNA sequences with SpCas9 nickase is an efficient method of gene editing that prevents unwanted indels from forming.
Product | Vector | Selection |
SpCas9 Nickase Plasmids | Plasmid Lentiviral |
Amp Amp, Puro Amp, GFP |
SaCas9 Plasmids
The Staphylococcus aureus Cas9 orthologue (SaCas9) is the preferred endonuclease for adeno-associated virus (AAV) applications. SaCas9 is approximately 1 kb shorter than SpCas9, and offers additional flexibility around AAV packaging constraints. The lower immunogenicity of AAV vectors makes SaCas9 well-suited for in vivo editing applications and therapeutics.
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Product | Vector | Selection |
SaCas9 Plasmids | AVV | Amp |
Transcription Activation (SAM) Plasmids
The CRISPR/Cas9 Synergistic Activation Mediator (SAM) system has been engineered to enable transcriptional activation of downstream targets. The SAM system utilizes three different activators, VP64, P65, and HSF1, which are assembled onto a catalytically dead Cas9 (dCas9) complex to drive transcription.
More information »
Product | Vector | Selection |
SAM gRNA Plasmids | Plasmid Lentiviral |
Amp Amp, Zeo |
SAM dCas9-VP64 Plasmids | Lentiviral | Amp, Blast Amp, GFP |
SAM MS2-P65-HSF1 Plasmids | Lentiviral | Amp, GFP Amp, Hygro |
Broad Institute Plasmid Collection
Broad Institute Plasmids are generated by the Broad Institute of Harvard and MIT. These plasmids contain a 17bp-1.8kb expressible linker in lieu of a customized sgRNA sequence, which can be modified by your laboratory.
Product | Vector | Selection |
eSpCas9 and SpCas9 Broad Plasmid Collection | Plasmid Lentiviral |
Amp, Puro Amp, GFP |
SpCas9 Nickase Broad Plasmid Collection |
Plasmid | Amp Amp, Puro Amp, GFP |
SaCas9 Broad Plasmid Collection |
AAV | Amp |
CRISPR Plasmid Detail
Service | Vector | Cas9/gRNA Expression | Delivery Method | Vector Type | Selection Marker |
eSpCas9 Plasmids | eSpCas9-2A-GFP (PX458 | eSpCas9 & gRNA | Plasmid | All-in-one Vector | AmpR EGFP |
eSpCas9 Plasmids | eSpCas9-2A-Puro (PX459) V2. | eSpCas9 & gRNA | Plasmid | All-in-one Vector | AmpR PuroR |
eSpCas9 Plasmids | eSpCas9-LentiCRISPR v | eSpCas9 & gRNA | Lentiviral | All-in-one Vector | AmpR PuroR |
SpCas9 Plasmids | pSpCas9 BB-2A-GFP PX458 | SpCas9 & gRNA | Plasmid | All-in-one Vector | AmpR EGFP |
SpCas9 Plasmids | pSpCas9 BB-2A-Puro (PX459) v2.0 | SpCas9 & gRNA | Plasmid | All-in-one Vector | AmpR PuroR |
SpCas9 Plasmids | pLentiCRISPR v2 | SpCas9 & gRNA | Lentiviral | All-in-one Vector | AmpR PuroR |
SpCas9 Plasmids SpCas9 Nickase Plasmids |
pLentiGuide-Puro | gRNA Only | Lentiviral | Dual Vector | AmpR PuroR |
SpCas9 Plasmids | pLentiCas9-Blast | SpCas9 Only | Lentiviral | Dual Vector | AmpR BsdR BleoR |
SpCas9 Plasmids | pLentiCas9-EGFP | SpCas9 Only | Lentiviral | Dual Vector | AmpR EGFP |
SpCas9 Plasmids | pGS-gRNA | gRNA Only | Plasmid | Dual Vector | AmpR |
SpCas9 Plasmids | pGS-gRNA-Neo | gRNA Only | Plasmid | Dual Vector | AmpR NeoR |
SpCas9 Plasmids | pSpCas9 PX165 | SpCas9 Only | Plasmid | Dual Vector | AmpR |
SpCas9 Plasmids | pAAV_SpGuide acceptor (PX552) | gRNA Only | AAV | Dual Vector | AmpR EGFP |
SpCas9 Plasmids | pAAV-SpCas9 PX551 | SpCas9 Only | AAV | Dual Vector | AmpR |
SpCas9 Nickase Plasmids | pSpCas9n BB PX460 | SpCas9 Nickase & gRNA | Plasmid | All-in-one Vector | AmpR |
SpCas9 Nickase Plasmids | pSpCas9n BB-2A-GFP PX461 | SpCas9 Nickase & gRNA | Plasmid | All-in-one Vector | AmpR EGFP |
SpCas9 Nickase Plasmids | pSpCas9n BB-2A-Puro (PX462) V2.0 | SpCas9 Nickase & gRNA | Plasmid | All-in-one Vector | AmpR PuroR |
SpCas9 Nickase Plasmids | pLentiCas9n-Blast | SpCas9 Nickase Only | Lentiviral | Dual Vector | AmpR BsdR BleoR |
SaCas9 Plasmids | pX601_AAV | SaCas9 & gRNA | AAV | All-in-one Vector | AmpR |
Transcription Activation (SAM) | pSgRNA(MS2) | gRNA Only | Plasmid | SAM Multi Vector | AmpR |
Transcription Activation (SAM) | pLenti_sgRNA(MS2)_zeo | gRNA Only | Lentiviral | SAM Multi Vector | AmpR ZeoR BeloR |
Transcription Activation (SAM) | pLenti_dCas9-VP64_Blast | Cas9 Activator | Lentiviral | SAM Multi Vector | AmpR BlastR BleoR |
Transcription Activation (SAM) | pLenti_dCas9-VP64_GFP | Cas9 Activator | Lentiviral | SAM Multi Vector | AmpR EGFP BleoR |
Transcription Activation (SAM) | pLenti_MS2-P65-HSF1_Hygro | Activator Adapter | Lentiviral | Multi Vector | AmpR HygroR BleoR |
Transcription Activation (SAM) | pLenti_MS2-P65-HSF1_GFP | Activator Adapter | Lentiviral | Multi Vector | AmpR EGFP BleoR |