Project Description
WESTAR-ONE EXTREME
제품 소개
Cyanagen 사의 WESTAR-ONE은 하나의 솔루션으로 이루어진 웨스턴블랏 (Western blot) 용 Chemiluminescent substrates입니다.
WESTAR-ONE 라인에는 pictogram 에서 low femtogram에 이르기까지 감도 수준이 다른 세 가지 제품이 포함되어 있어 대부분의 요구 사항을 충족합니다.
독점 기술을 기반으로 하는 WESTAR-ONE 사전 혼합 솔루션은 2 가지 용액을 사용하면서 발생할 수 있는 오염의 위험을 줄임으로써 결과의 일관성을 높일 수 있습니다.
WESTAR-ONE EXTREME is the most sensitive premixed chemiluminescent HRP substrate for low femtogram detection level in Western Blot that has been developed to date.
Because of its high signal strength, it can detect very small amounts of proteins while minimizing the amount of antibodies required.
제품사양
Description | WESTAR-ONE EXTREME |
Features | Low-femtogram detection No mixing is required Low antibody consumption to save your money Ready-to-use at RT |
Substrate type | HRP (Horseradish Peroxidase) Substrate |
Components | Luminol derivative/enhancer/peroxide solution |
Antibody Dilution Range | Primary: 1:5000 – 1:100000 Secondary: 1:100000 – 1:500000 (from 1 mg/mL stock solution) |
Available as | 250 mL kit (2,500 cm2 of membrane) |
Signal duration | 2 hours |
Storage | Stable for 1 year at RT |
Shipping | RT |
Application
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Westar ONE Extreme Comparison with its competitors Experimental contitions: Whole cell lysates: Neuroendocrine cell-line Amount of sample (μg): 20 µg protein Primary Antibodies: Anti-FAF1 (E-4) (sc-393965) : 75-80 kDa, 1:20000 Anti-PCNA (Cellsignaling #2586): 1:100000 (36 kDa) Anti-p21 Waf1/Cip1 (BD 610233) : 1:1000 Secondary Antibodies: HAM (Cellsignaling) = 1:25000 Imager: INTAS Chemocam |
WESTAR-ONE EXTREME Chemiluminescent substrates Selection guide
Product | Competitors | When to use |
WESTAR-ONE BASIC Cod. XLSU177 |
Amersham™ ECL-GE healthcare Amersham™ ECL start-GE healthcare Pierce™ ECL-Thermo scientific™ Immobilon® classico-Millipore™ |
Entry-level substrate for Very abundant proteins |
WESTAR-ONE PLUS Cod. XLSU178 |
Clarity™-Bio-rad Supersignal™ west dura-Thermo scientific™ Amersham™ ecl™ prime-GE healthcare Supersignal™ west pico plus-Thermo scientific™ Immobilon® crescendo-Millipore™ Westernbright™ quantum™-Advansta |
Everyday applications, Improved sensitivity over Base level ECL |
WESTAR-ONE EXTREME Cod. XLSU180 |
Immobilon® forte-Millipore™ Clarity max™-Bio-rad Supersignal™ west Femto-Thermo scientific™ Amersham™ ECL select™-GE healthcare Westernbright™ sirius™-Advansta Western lightning™ultra-PerkinElmer |
Target is the least abundant, with very high sensitivity |
주문정보
Cat. No. | 품명 | 규격 | 제조사 | 비고 |
XLSU177 | WESTAR-ONE BASIC | 250 mL | Cyanagen | Mid-picogram |
XLSU178 | WESTAR-ONE PLUS | 250 mL | Cyanagen | Mid-femtogram |
XLSU180 | WESTAR-ONE EXTREME | 250 mL | Cyanagen | Low-femtogram |
Document
제품 자료 | Upload date: 2022.04.06 | |
제품 매뉴얼 | WESTAR-and-WESTAR-ONE-Product-Manual.pdf | Upload date: 2022.04.06 |
MSDS_XLSU180_WESTAR-ONE-Extreme.pdf | Upload date: 2022.04.06 |