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제품 소개

Cyanagen 사의 WESTAR-ONE은 하나의 솔루션으로 이루어진 웨스턴블랏 (Western blot) 용 Chemiluminescent substrates입니다.
WESTAR-ONE 라인에는 pictogram 에서 low femtogram에 이르기까지 감도 수준이 다른 세 가지 제품이 포함되어 있어 대부분의 요구 사항을 충족합니다.
독점 기술을 기반으로 하는 WESTAR-ONE 사전 혼합 솔루션은 2 가지 용액을 사용하면서 발생할 수 있는 오염의 위험을 줄임으로써 결과의 일관성을 높일 수 있습니다.

WESTAR-ONE EXTREME is the most sensitive premixed chemiluminescent HRP substrate for low femtogram detection level in Western Blot that has been developed to date.
Because of its high signal strength, it can detect very small amounts of proteins while minimizing the amount of antibodies required.


• 모든 화학발광(chemiluminescence)이미징장비와 호환되며 X-ray film detection도 가능
• 다양한 유형의 membrane과 blocking reagent 및 광범위한 항체 희석에 적합
• 낮은 background 및 높은 S/N ratio를 위해 최적화
• 혼합 과정 불필요
• 낮은 항체 소비량
• 실온에서 바로 사용 가능


Features Low-femtogram detection
No mixing is required
Low antibody consumption to save your money
Ready-to-use at RT
Substrate type HRP (Horseradish Peroxidase) Substrate
Components Luminol derivative/enhancer/peroxide solution
Antibody Dilution Range Primary: 1:5000 – 1:100000
Secondary: 1:100000 – 1:500000
(from 1 mg/mL stock solution)
Available as 250 mL kit (2,500 cm2 of membrane)
Signal duration 2 hours
Storage Stable for 1 year at RT
Shipping RT


Westar ONE Extreme Comparison with its competitors Experimental contitions: Whole cell lysates: Neuroendocrine cell-line Amount of sample (μg): 20 µg protein Primary Antibodies: Anti-FAF1 (E-4) (sc-393965) : 75-80 kDa, 1:20000 Anti-PCNA (Cellsignaling #2586): 1:100000 (36 kDa) Anti-p21 Waf1/Cip1 (BD 610233) : 1:1000 Secondary Antibodies: HAM (Cellsignaling) = 1:25000 Imager: INTAS Chemocam

WESTAR-ONE EXTREME Chemiluminescent substrates Selection guide

Product Competitors When to use
Cod. XLSU177
Amersham™ ECL-GE healthcare
Amersham™ ECL start-GE healthcare
Pierce™ ECL-Thermo scientific™
Immobilon® classico-Millipore™
Entry-level substrate for Very abundant proteins
Cod. XLSU178
Supersignal™ west dura-Thermo scientific™
Amersham™ ecl™ prime-GE healthcare
Supersignal™ west pico plus-Thermo scientific™
Immobilon® crescendo-Millipore™
Westernbright™ quantum™-Advansta
Everyday applications, Improved sensitivity over Base level ECL
Cod. XLSU180
Immobilon® forte-Millipore™
Clarity max™-Bio-rad
Supersignal™ west Femto-Thermo scientific™
Amersham™ ECL select™-GE healthcare
Westernbright™ sirius™-Advansta
Western lightning™ultra-PerkinElmer
Target is the least abundant, with very high sensitivity


Cat. No. 품명 규격 제조사 비고
XLSU177 WESTAR-ONE BASIC 250 mL Cyanagen Mid-picogram
XLSU178 WESTAR-ONE PLUS 250 mL Cyanagen Mid-femtogram
XLSU180 WESTAR-ONE EXTREME 250 mL Cyanagen Low-femtogram


제품 자료 Upload date: 2022.04.06
제품 매뉴얼 WESTAR-and-WESTAR-ONE-Product-Manual.pdf Upload date: 2022.04.06
MSDS_XLSU180_WESTAR-ONE-Extreme.pdf Upload date: 2022.04.06