VeriFi Polymerase & Mixes

Figure 1. Versatility across a broad range of amplicon lengths
PCRBIO VeriFi Polymerase amplifies the range of fragment lengths indicated with high yield and specificity. The starting template amount is 4-30ng of mouse or human genomic DNA, diluted 1.5 to 3 fold. GC content ranges from 37-55%. L:PCRBIO Ladder II.

Figure 2. Increased success rates with complex templates
Amplification of a 17.5kb fragment of the HBB gene. The starting template amount is 150ng (A and C) and 30ng (B and D) of human genomic DNA, diluted 2 fold. A 2-step PCR protocol was used with amplification at 72°C (A and B) or 68°C (C and D). GC content is 37%. PCRBIO VeriFi Polymerase amplifies long fragments with yields comparable to Takara PrimeSTAR GXL DNA Polymerase. L: PCRBIO Ladder II.

Figure 3. Amplification of targets with high sensitivity and specificity compared to leading competitors
Amplification of a 1.0kb fragment of the GAPDH gene with different starting template amounts of mouse genomic DNA. A: 20ng, B: 3.2ng, C:
0.5ng, D: 0.08ng. GC content is 51%. L: PCRBIO Ladder IV. The reactions were set up following manufacturers’ recommendations. Cycling conditions were 95°C 2 min, then 30 cycles of 98°C 15 sec, 66°C 15 sec and 72°C 30 sec. PCRBIO VeriFi Polymerase displays greater sensitivity and specificity compared to leading competitors.