Project Description
VeriFi Polymerase & Mixes
제품 소개
PCRBIO VeriFi Polymerase는 cloning, site-directed mutagenesis 및 sequencing과 같은 더 높은 정확성이 요구되는 모든 PCR applications을 위해 설계된 high fidelity enzyme입니다.
이 enzyme은 3’-5’ exonuclease (proofreading) 활성을 위한 Pfu DNA polymerase 로부터 유래한 것입니다.
또한 dNTP, Mg 및 enhancer가 포함된 고급 buffer system이 제공되어 광범위한 target의 high fidelity PCR이 가능합니다.
제품특징
• Taq DNA 중합 효소보다 100 배 이상 높은 충실도
• 17.5kb 이상의 복잡한 genomic templates 에서도 증가된 PCR 성공률을 보임
• GC and AT rich sequence를 포함하여 까다로운 템플릿으로부터 효율적으로 증폭 가능
• 표준 PCR 조건 (10-30 초 / kb)에서 높은 수율
• GC bias감소를 위한 고온(~100°C) 사이클링
응용분야
• Long range PCR
• High fidelity PCR
• Site-directed mutagenesis
• Cloning
• Next Generation Sequencing
보관온도
• -20℃
Figure 1. Versatility across a broad range of amplicon lengths
PCRBIO VeriFi Polymerase amplifies the range of fragment lengths indicated with high yield and specificity. The starting template amount is 4-30ng of mouse or human genomic DNA, diluted 1.5 to 3 fold. GC content ranges from 37-55%. L:PCRBIO Ladder II.
Figure 2. Increased success rates with complex templates
Amplification of a 17.5kb fragment of the HBB gene. The starting template amount is 150ng (A and C) and 30ng (B and D) of human genomic DNA, diluted 2 fold. A 2-step PCR protocol was used with amplification at 72°C (A and B) or 68°C (C and D). GC content is 37%. PCRBIO VeriFi Polymerase amplifies long fragments with yields comparable to Takara PrimeSTAR GXL DNA Polymerase. L: PCRBIO Ladder II.
Figure 3. Amplification of targets with high sensitivity and specificity compared to leading competitors
Amplification of a 1.0kb fragment of the GAPDH gene with different starting template amounts of mouse genomic DNA. A: 20ng, B: 3.2ng, C:
0.5ng, D: 0.08ng. GC content is 51%. L: PCRBIO Ladder IV. The reactions were set up following manufacturers’ recommendations. Cycling conditions were 95°C 2 min, then 30 cycles of 98°C 15 sec, 66°C 15 sec and 72°C 30 sec. PCRBIO VeriFi Polymerase displays greater sensitivity and specificity compared to leading competitors.
주문정보
Cat. No. | 품명 | 규격 | 제조사 | 비고 |
PB10.42-01 | VeriFi Polymerase | 100 Units | PCRbiosystems | [1 x 0.05mL 2u/µL] & [1 x 1.7mL buffer] |
PB10.42-05 | VeriFi Polymerase | 500 Units | PCRbiosystems | [1 x 0.250mL 2u/µL] & [3 x 1.7mL buffer] |
PB10.43-01 | VeriFi Mix | 100 x 50µL Reactions | PCRbiosystems | 2 x 1.25mL |
PB10.43-05 | VeriFi Mix | 500 x 50µL Reactions | PCRbiosystems | 2 x (5 x 1.25mL) |
PB10.44-01 | VeriFi Mix Red | 100 x 50µL Reactions | PCRbiosystems | 2 x 1.25mL |
PB10.44-05 | VeriFi Mix Red | 500 x 50µL Reactions | PCRbiosystems | 2 x (5 x 1.25mL) |
PB10.44-480 | VeriFi PreMix, 8strip | 480 rxns | PCRbiosystems | 8 strip X 12 x 5 |
PB10.44-480TW | VeriFi PreMix, 12 strip | 480 rxns | PCRbiosystems | 12 strip X 12 x 5 |
Document
제품 자료 | PCRBIO-VeriFi-Polymerase-Product-Flyer.pdf | Upload date: 2021.07.12 |
제품 매뉴얼 | PB10.42-PCRBIO-VeriFi-Polymerase-Manual-1.pdf PB10.44-PCRBIO-VeriFi-Mix-Red-Manual-1.pdf PB10.43-PCRBIO-VeriFi-Mix-Manual-1.pdf |
Upload date: 2021.07.12 |
제품 MSDS | PB10.42-PCRBIO-VeriFi-Polymerase-MSDS.pdf PB10.43-PCRBIO-VeriFi-Mix-MSDS.pdf PB10.44-PCRBIO-VeriFi-Mix-Red-MSDS.pdf |
Upload date: 2021.07.12 |