Figure 3. Amplification of targets with high sensitivity and specificity compared to leading competitors
Amplification of a 1.0kb fragment of the GAPDH gene with different starting template amounts of mouse genomic DNA. A: 20ng, B: 3.2ng, C:
0.5ng, D: 0.08ng. GC content is 51%. L: PCRBIO Ladder IV. The reactions were set up following manufacturers’ recommendations. Cycling conditions were 95°C 2 min, then 30 cycles of 98°C 15 sec, 66°C 15 sec and 72°C 30 sec. PCRBIO VeriFi Polymerase displays greater sensitivity and specificity compared to leading competitors.