VeriFi Library Amplification Mix

제품 특징

•낮은 GC bias, 높은 GC/AT 표적에 이상적
•우수한 데이터 품질을 위한 더 많은 판독
•최대 감도 및 특이도를 위한 AptaLock hot start technology
•Taq DNA 중합효소보다 100배 더 높은 충실도
•실온에서 실험조건 설정 가능
•최소한의 피펫팅을 위한 2x ready mix

Applications

• NGS library amplification
• Whole Genome Sequencing
• RNA Seq
• Multiplex and high throughput PCR
• GC/AT-rich target sequencing

Figure 1. The higher number of unique reads per dataset
The number of uniquely mapped reads for three microbial genomes with different average GC content (E. coli ~50% GC, S. aureus ~30% GC, and S. griseus ~70% GC) is shown as a percentage total reads in four sequencing datasets. Datasets were generated using Illumina® sequencing in a blind experiment where all three genome libraries were amplified with different proofreading polymerases, VeriFi™ Libary Amplification Mix (purple), KAPA HiFi HotStart Library Amplification Kit (green), NEBNext® Ultra™ II Q5® Master Mix (orange), and Takara SeqAmp™ DNA Polymerase (blue). NGS library amplification with VeriFi™ Libary Amplification Mix leads to more unique reads per dataset after read deduplication compared to leading competitors.

Figure 2. Reduced GC bias of VeriFi™ Library Amplification Mix
Quantification of synthetic 1 kb sequences with different GC content, 30% GC (grey), 50% GC (purple), and 70% GC (blue). Amplification curves are shown on the left panel and melt peaks are on the right panel. Serial dilutions of each template were used in a reaction volume of 20 µL. Mixes tested are A) VeriFi™ Library Amplification Mix, B) KAPA HiFi HotStart Library Amplification Kit, C) NEBNext® Ultra™ II Q5® Master Mix, D) repliQa HiFi ToughMix (Quantabio). Each mix was run under cycling conditions recommended by the manufacturer. VeriFi™ Library Amplification Mix consistently amplifies templates across a broad range of GC content and over a wide range of concentrations with much less bias than competitors.

Figure 3. The number of uniquely mapped reads for three microbial genomes with different average GC content (E. coli ~50% GC, S. aureus ~30% GC, and S. griseus ~70% GC) is shown as a percentage total reads in four sequencing datasets. Datasets were generated using Illumina® sequencing in a blind experiment where all three genome libraries were amplified with different proofreading polymerases, VeriFi™ Library Amplification Mix (purple), KAPA HiFi HotStart Library Amplification Kit (green), NEBNext® Ultra™ II Q5® Master Mix (orange), and Takara SeqAmp™ DNA Polymerase (blue). NGS library amplification with VeriFi™ Library Amplification Mix leads to more unique reads per dataset after read deduplication compared to leading competitors.