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UltraScript 2.0 cDNA Synthesis Kit

제품 소개

UltraScript 2.0 역전사 효소는 견고하고 고열 안정성을 지닌 변형된 MMLV 역전사 효소로서 GC가 풍부하고 낮은 농도의 템플릿을 포함하여,
광범위한 RNA 샘플 유형으로부터 탁월한 cDNA 합성 속도와 높은 수율을 얻기 위해 설계되었습니다.

제품특징

•  55 ° C ~ 65 ° C 이상온도에서 작동하는 고 내열성 역전사 효소
• Advanced RNase inhibitor
• 최소 20pg 의 total RNA에서도 높은 cDNA 수율 확보
• GC가 풍부하고 고도로 구조화 된 template 에서도 정확한 역전사
• Sensitive detection of low copy number transcripts
• Reduced RNase H activity
• Mg 및 dNTP를 포함한  advanced buffer

응용분야

• cDNA synthesis for qPCR and PCR analysis, cloning, cDNA library preparation, and NGS
• Viral RNA targets
• miRNA targets
• Efficient synthesis from total RNA or poly(A)+ RNA

보관온도

•-20℃

Figure 1. UltraScript 2.0 Reverse Transcriptase maintains higher specific activity at elevated temperatures when compared to competing products and our original UltraScript Reverse Transcriptase. Specific activity is measured at the given incubation temperatures using an RT-qPCR assay.

Figure 2. Mouse liver total RNA was reverse transcribed using UltraScript 2.0 Reverse Transcriptase, followed by amplification of G-Act cDNA with qPCRBIO SyGreen Mix. UltraScript 2.0 Reverse Transcriptase can transcribe up to 3.5µg of RNA while retaining a linear response.

Figure 3. Mouse liver total RNA was reverse transcribed using UltraScript 2.0 Reverse Transcriptase, followed by amplification of G-Act cDNA using qPCRBIO SyGreen Mix. Up to 65°C, UltraScript 2.0 Reverse Transcriptase shows little change in yield with ΔC t values within ± 1 C t range (3b) and remains partially active up to 90°C (3a and 3b).

Figure 4. UltraScript 2.0 Reverse Transcriptase gives similar amounts of product across a wide range of temperatures in endpoint RT-PCR. Mouse reference RNA was reverse transcribed using UltraScript 2.0 Reverse Transcriptase. G-Act cDNA was amplified using qPCRBIO SyGreen Mix and visualized on EtBr 1% agarose gel.

주문정보

​ 제품번호 품명 규격 제조사 비고
PB30.31-02 UltraScript 2.0 cDNA Synthesis Kit 25 rxns PCR biosystems
PB30.31-10 UltraScript 2.0 cDNA Synthesis Kit 100 rxns
PB30.33-02 UltraScript 2.0 Reverse Transcriptase 10,000 units
PB30.33-10 UltraScript 2.0 Reverse Transcriptase 40,000 units

관련 논문

qPCRBIO cDNA Synthesis Kit

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Document

제품 자료 cDNA Synthesis Kit Flyer.pdf Download Size : 490kB
제품 MSDS PB30-MSDS.pdf Download Size : 321kB
제품 매뉴얼 cDNA Synthesis Kit Manual.pdf Download Size : 75.6kB