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UltraScript 2.0 Reverse Transcriptase & Kits

제품 소개

Real time PCR을 이용한 유전자 발현, 유전자 복제, cDNA 라이브러리의 생산과 같은 많은 분자생물학적인 연구 과정의 첫 번째 단계는 RNA를 상보적 DNA로 역전사 하는 것입니다.
이 과정에서 높은 GC 함량이나 복잡한 2차 구조를 가진 RNA의 경우 생각보다 진행이 더 어려울 수 있습니다.
복잡한 2차 구조를 가진 주형에서는 역전사효소가 RNA 템플릿에서 멈추거나, 분리되거나, 헤어핀 루프 부분을 건너뛰어 내부 결실로 인해 cDNA가 짧아질 수 있기 때문입니다.

이와 같이 까다로운 RNA 샘플로 작업할 때는 내열성이 뛰어난 UltraScript 2.0 Reverse Transcriptase를 권장합니다.
UltraScript 2.0 역전사 효소는 견고하고 고열 안정성을 지닌 변형된 MMLV 역전사 효소로서 GC가 풍부하고 낮은 농도의 템플릿을 포함하여, 광범위한 RNA 샘플 유형으로부터 탁월한 cDNA 합성 속도와 높은 수율을 얻기 위해 설계되었습니다.

제품특징

•  55 ° C ~ 65 ° C 이상온도에서 작동하는 고 내열성 역전사 효소
• Advanced RNase inhibitor
• 최소 20pg 의 total RNA에서도 높은 cDNA 수율 확보
• GC가 풍부하고 고도로 구조화 된 template 에서도 정확한 역전사
• Sensitive detection of low copy number transcripts
• Reduced RNase H activity
• Mg 및 dNTP를 포함한  advanced buffer

응용분야

• cDNA synthesis for qPCR and PCR analysis, cloning, cDNA library preparation, and NGS
• Viral RNA targets
• miRNA targets
• Efficient synthesis from total RNA or poly(A)+ RNA

보관온도

•-20℃

Figure 1. UltraScript 2.0 Reverse Transcriptase maintains higher specific activity at elevated temperatures when compared to competing products and our original UltraScript Reverse Transcriptase. Specific activity is measured at the given incubation temperatures using an RT-qPCR assay.

Figure 2. Mouse liver total RNA was reverse transcribed using UltraScript 2.0 Reverse Transcriptase, followed by amplification of G-Act cDNA with qPCRBIO SyGreen Mix. UltraScript 2.0 Reverse Transcriptase can transcribe up to 3.5µg of RNA while retaining a linear response.

Figure 3. Mouse liver total RNA was reverse transcribed using UltraScript 2.0 Reverse Transcriptase, followed by amplification of G-Act cDNA using qPCRBIO SyGreen Mix. Up to 65°C, UltraScript 2.0 Reverse Transcriptase shows little change in yield with ΔC t values within ± 1 C t range (3b) and remains partially active up to 90°C (3a and 3b).

Figure 4. UltraScript 2.0 Reverse Transcriptase gives similar amounts of product across a wide range of temperatures in endpoint RT-PCR. Mouse reference RNA was reverse transcribed using UltraScript 2.0 Reverse Transcriptase. G-Act cDNA was amplified using qPCRBIO SyGreen Mix and visualized on EtBr 1% agarose gel.

주문정보

​ 제품번호 품명 규격 제조사 비고
PB30.31-02 UltraScript 2.0 cDNA Synthesis Kit 25 rxns PCR biosystems [1 x 25μl UltraScript 2.0] & [1 x 100μl reaction mix
PB30.31-10 UltraScript 2.0 cDNA Synthesis Kit 100 rxns PCR biosystems [1 x 100μl UltraScript 2.0] & [4 x 100μl reaction mix]
PB30.32-02 UltraScript 2.0 cDNA Synthesis Kit Separate Oligos 25 rxns PCR biosystems [1 x 25μl UltraScript 2.0] & [1 x 200μl buffer] & [1 x 100μl
Anchored Oligo(dT)18] & [1 x 100μl Random Hexamers]
PB30.32-10 UltraScript 2.0 cDNA Synthesis Kit Separate Oligos 100 rxns PCR biosystems [1 x 100μl UltraScript 2.0] & [2 x 200μl buffer] & [1 x 100μl
Anchored Oligo(dT)18] & [1 x 100μl Random Hexamers]
PB30.33-01 UltraScript 2.0 Reverse Transcriptase 10,000 units PCR biosystems [2 x 25μl UltraScript 2.0, 200 u/μl] & [1 x 200μl buffer]
PB30.33-10 UltraScript 2.0 Reverse Transcriptase 40,000 units PCR biosystems [2 x 100μl UltraScript 2.0, 200 u/μl] & [4 x 200μl buffer]

관련 논문

qPCRBIO cDNA Synthesis Kit

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