Project Description
UltraScript 2.0 Reverse Transcriptase & Kits
제품 소개
Real time PCR을 이용한 유전자 발현, 유전자 복제, cDNA 라이브러리의 생산과 같은 많은 분자생물학적인 연구 과정의 첫 번째 단계는 RNA를 상보적 DNA로 역전사 하는 것입니다.
이 과정에서 높은 GC 함량이나 복잡한 2차 구조를 가진 RNA의 경우 생각보다 진행이 더 어려울 수 있습니다.
복잡한 2차 구조를 가진 주형에서는 역전사효소가 RNA 템플릿에서 멈추거나, 분리되거나, 헤어핀 루프 부분을 건너뛰어 내부 결실로 인해 cDNA가 짧아질 수 있기 때문입니다.
이와 같이 까다로운 RNA 샘플로 작업할 때는 내열성이 뛰어난 UltraScript 2.0 Reverse Transcriptase를 권장합니다.
UltraScript 2.0 역전사 효소는 견고하고 고열 안정성을 지닌 변형된 MMLV 역전사 효소로서 GC가 풍부하고 낮은 농도의 템플릿을 포함하여, 광범위한 RNA 샘플 유형으로부터 탁월한 cDNA 합성 속도와 높은 수율을 얻기 위해 설계되었습니다.
제품특징
• 55 ° C ~ 65 ° C 이상온도에서 작동하는 고 내열성 역전사 효소
• Advanced RNase inhibitor
• 최소 20pg 의 total RNA에서도 높은 cDNA 수율 확보
• GC가 풍부하고 고도로 구조화 된 template 에서도 정확한 역전사
• Sensitive detection of low copy number transcripts
• Reduced RNase H activity
• Mg 및 dNTP를 포함한 advanced buffer
응용분야
• cDNA synthesis for qPCR and PCR analysis, cloning, cDNA library preparation, and NGS
• Viral RNA targets
• miRNA targets
• Efficient synthesis from total RNA or poly(A)+ RNA
보관온도
•-20℃
Figure 1. UltraScript 2.0 Reverse Transcriptase maintains higher specific activity at elevated temperatures when compared to competing products and our original UltraScript Reverse Transcriptase. Specific activity is measured at the given incubation temperatures using an RT-qPCR assay.
Figure 2. Mouse liver total RNA was reverse transcribed using UltraScript 2.0 Reverse Transcriptase, followed by amplification of G-Act cDNA with qPCRBIO SyGreen Mix. UltraScript 2.0 Reverse Transcriptase can transcribe up to 3.5µg of RNA while retaining a linear response.
Figure 3. Mouse liver total RNA was reverse transcribed using UltraScript 2.0 Reverse Transcriptase, followed by amplification of G-Act cDNA using qPCRBIO SyGreen Mix. Up to 65°C, UltraScript 2.0 Reverse Transcriptase shows little change in yield with ΔC t values within ± 1 C t range (3b) and remains partially active up to 90°C (3a and 3b).
Figure 4. UltraScript 2.0 Reverse Transcriptase gives similar amounts of product across a wide range of temperatures in endpoint RT-PCR. Mouse reference RNA was reverse transcribed using UltraScript 2.0 Reverse Transcriptase. G-Act cDNA was amplified using qPCRBIO SyGreen Mix and visualized on EtBr 1% agarose gel.
주문정보
| 제품번호 | 품명 | 규격 | 제조사 | 비고 |
| PB30.31-02 | UltraScript 2.0 cDNA Synthesis Kit | 25 rxns | PCR biosystems | [1 x 25μl UltraScript 2.0] & [1 x 100μl reaction mix |
| PB30.31-10 | UltraScript 2.0 cDNA Synthesis Kit | 100 rxns | PCR biosystems | [1 x 100μl UltraScript 2.0] & [4 x 100μl reaction mix] |
| PB30.32-02 | UltraScript 2.0 cDNA Synthesis Kit Separate Oligos | 25 rxns | PCR biosystems | [1 x 25μl UltraScript 2.0] & [1 x 200μl buffer] & [1 x 100μl Anchored Oligo(dT)18] & [1 x 100μl Random Hexamers] |
| PB30.32-10 | UltraScript 2.0 cDNA Synthesis Kit Separate Oligos | 100 rxns | PCR biosystems | [1 x 100μl UltraScript 2.0] & [2 x 200μl buffer] & [1 x 100μl Anchored Oligo(dT)18] & [1 x 100μl Random Hexamers] |
| PB30.33-01 | UltraScript 2.0 Reverse Transcriptase | 10,000 units | PCR biosystems | [2 x 25μl UltraScript 2.0, 200 u/μl] & [1 x 200μl buffer] |
| PB30.33-10 | UltraScript 2.0 Reverse Transcriptase | 40,000 units | PCR biosystems | [2 x 100μl UltraScript 2.0, 200 u/μl] & [4 x 200μl buffer] |
관련 논문
qPCRBIO cDNA Synthesis Kit
Chandran, S., Watkins, J., Abdul‐Aziz, A., Shafat, M., Calvert, P.A., Bowles, K.M., Flather, M.D., Rushworth, S.A. and Ryding, A.D., 2017. Inflammatory Differences in Plaque Erosion and Rupture in Patients With ST‐Segment Elevation Myocardial Infarction. Journal of the American Heart Association, 6(5), p.e005868.
Czubala, M.A., 2015. Transforming growth factor-beta (TGF-β) induces HIV-1 restriction in Langerhans cells (Doctoral dissertation, Cardiff University). PhD supervisors: Prof. Vincent Piguet and Dr Fabien Blanchet
Eldar-Yedidia, Y., Bar-Meir, M., Hillel, M., Abitbol, G., Broide, E., Falk, R., Assous, M. and Schlesinger, Y., 2016. Low interferon relative-response to cytomegalovirus is associated with low likelihood of intrauterine transmission of the virus. PloS one, 11(2), p.e0147883.
Kim, H.S., Kim, J.H., Lee, J.Y., Yoon, Y.M., Kim, I.H., Yoon, H.S. and Youn, B.S., 2017. Small moleculemediated reprogramming of epithelial-mesenchymal transition thereby blocking fibrosis. bioRxiv, p.106591.
Lanfredini, S., Olivero, C., Borgogna, C., Calati, F., Powell, K., Davies, K.J., De Andrea, M., Harries, S., Tang, H.K.C., Pfister, H. and Gariglio, M., 2017. HPV8 field cancerization in a transgenic mouse model is due to Lrig1+ keratinocyte stem cell expansion. Journal of Investigative Dermatology.
Lee, A., Papangeli, I., Park, Y., Jeong, H.N., Choi, J., Kang, H., Jo, H.N., Kim, J. and Chun, H.J., 2017. A PPARγ-dependent miR-424/503-CD40 axis regulates inflammation mediated angiogenesis. Scientific Reports, 7.
Liew, J.W., Fong, M.Y. and Lau, Y.L., 2017. Quantitative real-time PCR analysis of Anopheles dirus TEP1 and NOS during Plasmodium berghei infection, using three reference genes. PeerJ, 5, p.e3577.
Perfecto, A., Elgy, C., Valsami-Jones, E., Sharp, P., Hilty, F. and Fairweather-Tait, S., 2017. Mechanisms of Iron Uptake from Ferric Phosphate Nanoparticles in Human Intestinal Caco-2 Cells. Nutrients, 9(4), p.359.
Piddock, R.E., Loughran, N., Marlein, C.R., Robinson, S.D., Edwards, D.R., Yu, S., Pillinger, G.E., Zhou, Z., Zaitseva, L., Auger, M.J., Rushworth, S.A. and Bowles, K.M., 2017. PI3Kδ and PI3Kγ isoforms have distinct functions in regulating pro-tumoural signalling in the multiple myeloma microenvironment. Blood cancer journal, 7(3), pp.e539-e539.
Prevo, R., Tiwana, G.S., Maughan, T.S., Buffa, F.M., McKenna, W.G. and Higgins, G.S., 2017. Depletion of Signal Recognition Particle 72kDa increases radiosensitivity. Cancer Biology & Therapy, (justaccepted), pp.00-00.
Repin, R.A.M., Mutalib, S.A., Shahimi, S., Khalid, R.M., Ayob, M.K., Bakar, M.F.A. and Isa, M.N.M., 2016, November. Bioinformatics analysis and detection of gelatinase encoded gene in Lysinibacillussphaericus. In AIP Conference Proceedings (Vol. 1784, No. 1, p. 030044). AIP Publishing.
Roura, M., Catalá, M.G., Soto-Heras, S., Hammami, S., Izquierdo, D., Fouladi-Nashta, A. and Paramio, M.T., 2017. Linoleic (LA) and linolenic (ALA) acid concentrations in follicular fluid of prepubertal goats and their effect on oocyte in vitro maturation and embryo development. Reproduction,Fertility and Development.
Schniete, J.K., Cruz-Morales, P., Selem, N., Fernandez-Martinez, L.T., Hunter, I.S., Barona-Gomez, F. and Hoskisson, P., 2017. Expanding Gene Families Helps Generate The Metabolic Robustness Required For Antibiotic Biosynthesis. bioRxiv, p.119354.
Simon, Á., Jávor, A., Bai, P., Oláh, J. and Czeglédi, L., 2017. Reference gene selection for reverse transcription quantitative polymerase chain reaction in chicken hypothalamus under different feeding status. Journal of Animal Physiology and Animal Nutrition.
Sipos, A., Iván, J., Bécsi, B., Darula, Z., Tamás, I., Horváth, D., Medzihradszky, K.F., Erdődi, F. and Lontay, B., 2017. Myosin phosphatase and RhoA-activated kinase modulate arginine methylation by the regulation of protein arginine methyltransferase 5 in hepatocellular carcinoma cells. Scientific reports, 7, p.40590.
Theerawatanasirikul, S., Pongket, P., Larsuprom, L. and Sastravaha, A., 2016. Atopic dog skin shows decrease of claudin-1 but increase of atopic signature cytokines. Turkish Journal of Veterinary and Animal Sciences, 40(2), pp.243-250.
Trovisco, V., Belaya, K., Nashchekin, D., Irion, U., Sirinakis, G., Butler, R., Lee, J.J., Gavis, E.R. and St Johnston, D., 2016. bicoid mRNA localises to the Drosophila oocyte anterior by random Dyneinmediated transport and anchoring. Elife, 5.
Ubaidillah, M., Safitri, F.A., Jo, J.H., Lee, S.K., Hussain, A., Mun, B.G., Chung, I.K., Yun, B.W. and Kim, K.M., 2016. Roles of plant hormones and anti-apoptosis genes during drought stress in rice (Oryza sativa L.). 3 Biotech, 6(2), p.247.
Ubaidillah, M., Safitri, F.A., Lee, S., Park, G.H. and Kim, K.M., 2015. Alteration of plant hormones in transgenic rice (Oryza sativa L.) by overexpression of anti-apoptosis genes during salinity stress. Journal of Plant Biotechnology, 42(3), pp.168-179.
Vu, B.T., Duong, Q.T.N., Le, P.M. and Van Pham, P., 2016. Culture and differentiation of cytokineinduced killer cells from umbilical cord blood-derived mononuclear cells. Biomedical Research and Therapy, 3(01), pp.460-468.
Wahab, R.M.A., Rozali, N.A.M., Senafi, S., Abidin, I.Z.Z., Ariffin, Z.Z. and Ariffin, S.H.Z., 2017. Impact of isolation method on doubling time and the quality of chondrocyte and osteoblast differentiated from murine dental pulp stem cells. PeerJ, 5, p.e3180.
Yip, H.Y., Poh, M.S.W. and Chia, Y.Y., 2016. The effects of glycyrrhizic acid and glabridin in the regulation of CXCL5 inflammation gene on acceleration of wound healing. Asian Pacific Journal of Tropical Biomedicine, 6(2), pp.108-113.
Document
| 제품 자료 | UltraScript 2.0 cDNA Synthesis Kit.pdf | Upload date: 2021.07.02 |
| 제품 매뉴얼 | PB30.31-UltraScript-2.0-cDNA-Synthesis-Kit-Manual.pdf PB30.32-UltraScript-2.0-cDNA-Synthesis-Kit-Separate-Oligos-Manual.pdf PB30.33-UltraScript-2.0-Reverse-Transcriptase-Manual.pdf |
Upload date: 2021.07.02 |
| 제품 MSDS | PB30-Products-MSDS.pdf | Upload date: 2021.07.02 |