Project Description

qPCRBIO SyGreen 1-Step Kits

제품 소개

qPCRBIO SyGreen 1-Step kit는 하나의 PCR tube 내에서 cDNA 합성과 real-time PCR이 가능하도록 최적화 시킨 제품입니다.
이 제품은 열에 안정한 modified MMLV reverse transcriptase와 Hot start polymerase, dNTPs, buffer, MgCl2, intercalating dye를 최적의 조건으로 혼합 한 제품입니다.

• qPCRBIO SyGreen 1-Step Detect kit는 low copy number template target에 대한 매우 높은 민감도를 갖도록 디자인
• qPCRBIO SyGreen 1-Step Go kit는 높은 template 농도로부터 빠르고 정확한 결과를 얻도록 디자인

제품특징

• qPCRBIO SyGreen 1-Step Detect: template amounts of 1pg – 10ng total RNA or >0.01pg mRNA per reaction
• qPCRBIO SyGreen 1-Step Go: template amounts of 10pg – 100ng total RNA or >0.01pg mRNA per reaction
• 매우 높은 민감도와 특이도
• 열에 안정한 modified MMLV RTase
• OneStep qRT-PCR

응용분야

• Absolute quantification
• Relative gene expression analysis
• Detection of extremely low copy number targets

보관온도

•-20℃

Shows amplification traces of the ACTG1 gene from a dilution series of total RNA extracted from mouse liver. Total RNA concentration varied from 25pg to 250ng per 20μl reaction. Cycling conditions were 45°C 10min for cDNA synthesis, followed by 95°C 2min hot start, then 45 cycles of 95°C 10sec, 60°C 10sec on Roche LC480. qPCRBIO SyGreen 1-Step Go had equal performance at high RNA concentrations and superior performance at lower RNA concentrations, displaying linear spacing between amplification curves, earlier amplification by 3–4 cycles, and lower prevalence of primer dimer.

Figure 2a. Comparison of qPCRBIO SyGreen 1-Step Detect (blue) and qPCRBIO SyGreen 1-Step Go (purple), showing ideal template ranges for each product. The ACTG1 gene was amplified from a dilution series of total RNA extracted from mouse liver. qPCRBIO SyGreen 1-Step Go (purple) shows efficient amplification of total RNA in the range 100pg to 100ng per reaction. qPCRBIO SyGreen 1-Step Detect (blue) shows efficient amplification of total RNA in the range 10pg to 10ng per reaction (figure 2a) on a Roche LC480. .

Figure 2b. Comparison of qPCRBIO SyGreen 1-Step Detect (blue) and qPCRBIO SyGreen 1-Step Go (purple), showing ideal template ranges for each product. The ACTG1 gene was amplified from a dilution series of total RNA extracted from mouse liver. qPCRBIO SyGreen 1-Step Go (purple) shows efficient amplification of total RNA in the range 100pg to 100ng per reaction. Cycling conditions were 45°C 10min for cDNA synthesis, followed by 95°C 2min hot start, then 45 cycles of 95°C 10sec, 60°C 30sec, concluding with a melt analysis (figure 2b) on a Roche LC480.

주문정보

Cat. No. 품명 규격 제조사 비고
PB25.11-01 qPCRBIO SyGreen 1-Step Detect Lo-ROX 100 rxns PCRbiosystems [1 x 1ml mix] & [1 x 200μl RTase]
PB25.11-03 qPCRBIO SyGreen 1-Step Detect Lo-ROX 300 rxns PCRbiosystems [3 x 1ml mix] & [3 x 200μl RTase]
PB25.12-01 qPCRBIO SyGreen 1-Step Detect Hi-ROX 100 rxns PCRbiosystems [1 x 1ml mix] & [1 x 200μl RTase]
PB25.12-03 qPCRBIO SyGreen 1-Step Detect Hi-ROX 300 rxns PCRbiosystems [3 x 1ml mix] & [3 x 200μl RTase]
PB25.31-01 qPCRBIO SyGreen 1-Step Go Lo-ROX 100 rxns PCRbiosystems [1 x 1ml mix] & [1 x 200μl RTase]
PB25.31-03 qPCRBIO SyGreen 1-Step Go Lo-ROX 300 rxns PCRbiosystems [3 x 1ml mix] & [3 x 200μl RTase]
PB25.32-01 qPCRBIO SyGreen 1-Step Go Hi-ROX 100 rxns PCRbiosystems [1 x 1ml mix] & [1 x 200μl RTase]
PB25.32-03 qPCRBIO SyGreen 1-Step Go Hi-ROX 300 rxns PCRbiosystems [3 x 1ml mix] & [3 x 200μl RTase]

관련 논문

qPCRBIO SyGreen 1-Step Detect

Huynh, O.T., Truong, M.T.H. and Van Pham, P., 2017. Experimental reprogramming of murine embryonic fibroblasts towards induced pluripotent stem cells using a single polycistronic vector. Progress in Stem Cell, 4(01), pp.159-174.

Orriss, I.R., Guneri, D., Hajjawi, M.O., Shaw, K., Patel, J.J. and Arnett, T.R., 2017. Activation of the P2Y2 receptor regulates bone cell function by enhancing ATP release. Journal of Endocrinology, 233(3), pp.341-356.

Tang, M.B., Chang, H.M., Wu, W.C., Chou, Y.C. and Yu, C.P., 2016. First Detection of Chicken Anemia Virus and Norovirus Genogroup II in Stool of Children with Acute Gastroenteritis in Taiwan. The Southeast Asian journal of tropical medicine and public health, 47(3), pp.416-423.

Vu, N.B., Le, H.T.N., Dao, T.T.T., Phi, L.T. and Phan, N.K., 2017. Allogeneic Adipose-Derived Mesenchymal Stem Cell Transplantation Enhances the Expression of Angiogenic Factors in a Mouse Acute Hindlimb Ischemic Model.

Wanka, F., Arentshorst, M., Cairns, T.C., Jørgensen, T., Ram, A.F. and Meyer, V., 2016. Highly active promoters and native secretion signals for protein production during extremely low growth rates in Aspergillus niger. Microbial cell factories, 15(1), p.145.

Wen, T.K., Tang, M.B. and Yu, C.P., 2016. Detection of Wu Polyomavirus and Norovirus Genogroup II in Stools of Children with Acute Gastroenteritis in Taiwan. Southeast Asian Journal of Tropical Medicine and Public Health, 47(1), p.23.

qPCRBIO SyGreen 1-Step Detect Lo-ROX

Al-Jazzar, A., Javaheri, B., Prideaux, M., Boyde, A., Scudamore, C.L., Cherifi, C., Hay, E., Hopkinson, M., Boyd, M., Cohen-Solal, M. and Farquharson, C., 2016. Dmp1 Promoter-Driven Diphtheria Toxin Receptor Transgene Expression Directs Unforeseen Effects in Multiple Tissues. International journal of molecular sciences, 18(1), p.29.

Charoensopharat, K., Thanonkeo, P., Thanonkeo, S. and Yamada, M., 2015. Ethanol production from Jerusalem artichoke tubers at high temperature by newly isolated thermotolerant inulin-utilizing yeast Kluyveromyces marxianus using consolidated bioprocessing. Antonie Van Leeuwenhoek, 108(1), pp.173-190.

Khumkarjorn, N., Thanonkeo, S., Yamada, M. and Thanonkeo, P., 2017. Cloning and expression analysis of a flavanone 3-hydroxylase gene in Ascocenda orchid. Journal of Plant Biochemistry and Biotechnology, 26(2), pp.179-190.

Nuanpeng, S., Thanonkeo, S., Yamada, M. and Thanonkeo, P., 2016. Ethanol production from sweet sorghum juice at high temperatures using a newly isolated thermotolerant yeast Saccharomyces cerevisiae DBKKU Y-53. Energies, 9(4), p.253.

Techaparin, A., Thanonkeo, P. and Klanrit, P., 2017. Gene expression profiles of the thermotolerant yeast Saccharomyces cerevisiae strain KKU-VN8 during high-temperature ethanol fermentation using sweet sorghum juice. Biotechnology Letters, pp.1-7.

​Van Trinh, N., Dang, N.D.P., Tran, D.H. and Van Pham, P., 2016. Taraxacum officinale dandelion extracts efficiently inhibited the breast cancer stem cell proliferation. Biomedical Research and Therapy, 3(7), pp.1-13.

qPCRBIO SyGreen 1-Step Detect Hi-ROX

Imparato, V., Santos, S.S., Johansen, A., Geisen, S. and Winding, A., 2016. Stimulation of bacteria and protists in rhizosphere of glyphosate-treated barley. Applied Soil Ecology, 98, pp.47-55.

Kulimushi, P.Z., Arias, A.A., Franzil, L., Steels, S. and Ongena, M., 2017. Stimulation of Fengycin-Type Antifungal Lipopeptides in Bacillus amyloliquefaciens in the Presence of the Maize Fungal Pathogen Rhizomucor variabilis. Frontiers in Microbiology, 8.

Molinatto, G., Franzil, L., Steels, S., Puopolo, G., Pertot, I. and Ongena, M., 2017. Key impact of an uncommon plasmid on Bacillus amyloliquefaciens subsp. plantarum S499 developmental traits and lipopeptide production. Frontiers in microbiology, 8.

Santos SS, Hendriksen NB, Jakobsen HH, Winding A. Effects of Bacillus cereus Endospores on FreeLiving Protist Growth. Microbial ecology. 2017 Apr 1;73(3):699-709.

Santos, S.S., Nielsen, T.K., Hansen, L.H. and Winding, A., 2015. Comparison of three DNA extraction methods for recovery of soil protist DNA. Journal of microbiological methods, 115, pp.13-19.

qPCRBIO SyGreen 1-Step Go

Huynh, O.T., Truong, M.T.H. and Van Pham, P., 2017. Experimental reprogramming of murine embryonic fibroblasts towards induced pluripotent stem cells using a single polycistronic vector. Progress in Stem Cell, 4(01), pp.159-174.

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