RiboShield™ RNase Inhibitor

제품특징

• 뛰어난 보호 기능을 통해 RNA에 민감한 애플리케이션의 성능 향상
• 특히 SARS-CoV-2 검출을 위한 saliva-based tests 에 적합
• eukaryotic RNases 억제 (RNase A, B and C)
• reverse transcriptases, RNA polymerases and Taq DNA polymerase 와 호환
• 65°C에서 최소 30분간 안정
• Ribonuclease and phosphatase free
• 샘플의 장기 보관에 이상적

응용분야

• cDNA synthesis
• 1-step RT-PCR
• 1-step RT-qPCR
• RNA purification
• RNA sequencing
• In vitro transcription and translation
• Saliva-based diagnostic testing for SARS-CoV-2

보관온도

• -20℃

FAQs

How much RiboShield™ should I add to my RNA samples for storage?
For sample storage, we recommend using RiboShield™ RNase inhibitor as a 100x solution (i.e., the concentration of RNase inhibitor in the storage buffer should be 0.4 U/μL). This final concentration can be exceeded without negatively affecting downstream enzymatic reactions but is generally unnecessary unless you suspect RNase contamination.

How to use RiboShield™ RNase inhibitor?
All PCRBIO products for 1-step and 2-step RT-PCR and RT-qPCR, as well as for cDNA synthesis, already contain the RNase inhibitor, so there is no need to add RiboShield™ RNase inhibitor when using these products.

If used with other kits, we recommend adding 1µL (40U) of RiboShield™ RNase inhibitor in a reaction mix of 20µL. In case the source of RNA is known to be rich in RNases, titration may be required to verify the complete protection of the ribonucleic acid.

What can RiboShield™ RNase inhibitor be used for?
RiboShield™ RNase inhibitor is designed for RNA-sensitive applications such as RT-qPCR, cDNA synthesis, and RNA-seq, where even small amounts of RNase can be detrimental to RNA quality and experimental outcome. In addition, it can also be added to extracted RNA to preserve RNA quality in areas with a high likelihood of RNAse contamination or to RNA extraction buffers such as those used in Pereira et al., 2010, to help improve the preservation of RNA integrity as you progress through your workflow.

(Pereira MJB, Behera V, Walter NG (2010) Nondenaturing Purification of Co-Transcriptionally Folded RNA Avoids Common Folding Heterogeneity. PLOS ONE 5(9): e12953. https://doi.org/10.1371/journal.pone.0012953)

What does RiboShield™ RNase inhibitor do?
RiboShield™ RNase inhibitor acts as a shield against the degradation of target RNA which can be caused by ribonuclease contamination of the RNA preparation.

What kind of RNases can be inhibited using RiboShield™ RNase inhibitor?
RiboShield™ RNase inhibitor is a protein-based inhibitor that non-covalently binds and blocks the activity of the most common and troublesome RNases, including eukaryotic RNases of the neutral type (e.g. RNases A, B, and C). It does not inhibit RNases T1, T2, U1, U2, CL3, RNase I and H.