Project Description

RiboShield™ RNase Inhibitor

제품 소개

RiboShield™ RNase Inhibitor는 ribonucleases의 활동을 차단하여 RNA를 RNase digestion 으로부터 안정적으로 보호하는 재조합 단백질입니다.
RT-qPCR, cDNA 합성 및 RNA 시퀀싱과 같은 RNA에 민감한 애플리케이션에 적합하도록 설계되었습니다.

제품특징

• 뛰어난 보호 기능을 통해 RNA에 민감한 애플리케이션의 성능 향상
• 특히 SARS-CoV-2 검출을 위한 saliva-based tests 에 적합
• eukaryotic RNases 억제 (RNase A, B and C)
• reverse transcriptases, RNA polymerases and Taq DNA polymerase 와 호환
• 65°C에서 최소 30분간 안정
• Ribonuclease and phosphatase free
• 샘플의 장기 보관에 이상적

응용분야

• cDNA synthesis
• 1-step RT-PCR
• 1-step RT-qPCR
• RNA purification
• RNA sequencing
• In vitro transcription and translation
• Saliva-based diagnostic testing for SARS-CoV-2

보관온도

• -20℃

Figure 1. Stable at high temperatures
RiboShield™ RNase inhibitor was incubated at the indicated temperatures for 30 minutes. 40U of the inhibitor were then added to 1μg RNA and 5pg RNase A in 5x UltraScript buffer and incubated at 37°C for 30 min. Samples were then loaded on a 1% agarose gel. RiboShield™ RNase Inhibitor can inhibit RNase A at temperatures up to 65°C for at least 30 minutes.

Figure 2. Superior protection against RNase A
RiboShield™ RNase Inhibitor and three competitor products (40U) were incubated with the indicated amounts of RNase A and 1μg RNA in 5x UltraScript buffer at 37°C for 30 min. Samples were then loaded on a 1% agarose gel. L: Ambion RNA Millennium Markers. The RNase inhibitors used were PCR Biosystems’  iboShield™, Promega’s RNasin®, Bioline’s RiboSafe and ThermoFisher’s RNaseOUT™. RiboShield™ RNase Inhibitor offers the greatest RNA protection amongst  the inhibitors tested.

주문정보

Cat. No. 품명 규격 제조사 비고
PB30.23-02 RiboShield™ RNase Inhibitor 2,500 Units PCRbiosystems 1 x 62.5µL
PB30.23-10 RiboShield™ RNase Inhibitor 10,000 Units PCRbiosystems 4 x 62.5µL

Document

제품 자료 RiboShield-RNase-Inhibitor-Flyer-1.pdf Upload date: 2021.07.12
제품 매뉴얼 PB30.23-RiboShield-RNase-Inhibitor-Manual.pdf Upload date: 2021.07.12
제품 MSDS PB30-Products-MSDS.pdf Upload date: 2021.07.12