RiboShield™ RNase Inhibitor

Figure 1. Stable at high temperatures
RiboShield™ RNase inhibitor was incubated at the indicated temperatures for 30 minutes. 40U of the inhibitor were then added to 1μg RNA and 5pg RNase A in 5x UltraScript buffer and incubated at 37°C for 30 min. Samples were then loaded on a 1% agarose gel. RiboShield™ RNase Inhibitor can inhibit RNase A at temperatures up to 65°C for at least 30 minutes.

Figure 2. Superior protection against RNase A
RiboShield™ RNase Inhibitor and three competitor products (40U) were incubated with the indicated amounts of RNase A and 1μg RNA in 5x UltraScript buffer at 37°C for 30 min. Samples were then loaded on a 1% agarose gel. L: Ambion RNA Millennium Markers. The RNase inhibitors used were PCR Biosystems’  iboShield™, Promega’s RNasin®, Bioline’s RiboSafe and ThermoFisher’s RNaseOUT™. RiboShield™ RNase Inhibitor offers the greatest RNA protection amongst  the inhibitors tested.