Project Description
Protein Purification Resins
제품 소개
단백질 정제는 관심있는 단백질의 기능, 구조 및 상호 작용을 이해하는데 매우 중요한 첫 단계입니다.
여러 정제 방법 중 affinity chromatography는 표적 단백질에 대해 높은 선택성, 고분해능 및 고용량을 제공할 수 있어 가장 강력한 정제 방법으로 활용되고 있습니다.
GenScript는 batch/gravity purification을 위한 다양한 종류의 affinity purification resins을 제공하여 native sample로부터 단백질과 항체를 효율적이고 편리하게 분리할 수 있게 도와 주고 있습니다.
제품 특징
• 단백질 및 항체 정제를 위한 우수한 affinity chromatography media
• batch or gravity-flow operation 을 위한 빠르고 간단한 과정
• 비특이적 결합 확률이 매우 낮아 타겟에 대한 높은 결합 능력을 보임
• 다양한 샘플 종류 및 버퍼와 호환
• 여러 번 재사용 가능하여 비용 효율성 향상
Applications
• Antibody purification
• His and GST tagged protein purification
• Purification of streptavidin, avidin, or their conjugates
• Purification of biotinylated protein, antibody, lectin, etc
Selection guide
| Application | Product | Ligand used | Binding capacity | Size | Cat.No. |
| Antibody purification | Protein A Resin | Recombinant protein A | >20 mg human IgG/ml settled resin | 5 ml | L00210 |
| Protein A Resin FF | Recombinant protein A on FPLC-compatible resin | > 40 mg human IgG/ml settled resin | 5 ml | L00464 | |
| Protein G Resin | Recombinant protein G | >20 mg sheep IgG/ml settled resin | 5 ml | L00209 | |
| Protein G Resin FF | Recombinant protein G on FPLC-compatible resin | ≥ 20 mg human IgG/m settled resin | 5/25/100 ml | L00664 | |
| Protein L Resin | Recombinant protein L | >15 mg rabbit IgG/ml settled resin | 2.5 ml | L00239 | |
| Alkaline stable antibody purification resin | Monofinity A Resin | Recombinant alkaline tolerant protein A | >30 mg human IgG/ml settled resin | 25/200 ml 1/5/10 L custom |
L00433 |
| Antibody purification (Prepacked Column) | Monofinity A Resin Prepacked Column | Recombinant alkaline tolerant protein A in a prepacked column | >30 mg human IgG/ml settled resin | 1/5/25 ml | L00433 |
| Protein A Resin FF Prepacked Column | Recombinant alkaline tolerant protein A on FPLC-compatible resin in a prepacked column | > 40 mg human IgG/ ml settled resin | 2 /5 /25 ml | L00680 | |
| Protein G Resin FF Prepacked Column | Recombinant protein G on FPLC-compatible resin in a prepacked column | ≥ 20 mg human IgG/ml settled resin | 2 /5 /25 ml | L00681 | |
| His-tagged protein purification | High Affinity Ni-charged Resin | Ni-NTA-charged resin | 45-60 mg 6xHis-tagged protein (27 kD) /ml settled resin | 10 ml 25 ml 500 ml |
L00223 |
| Ni Resin FF | Ni-IDA-charged FPLC-compatible resin | >40 mg 6xhistidine-tagged protein /ml settled resin | 5 ml 25 ml |
L00465 | |
| High Affinity Ni-Charged Resin FF | Ni-NTA-charged FPLC-compatible resin | >45 mg of histidine-tagged protein /ml settled resin | 5/25/100 ml | L00666 | |
| His-tagged protein purification (Prepacked Column) | High Affinity Ni-Charged Resin FF Prepacked Column | Ni-NTA-charged FPLC-compatible resin in a prepacked column | >45 mg of histidine-tagged protein /ml settled resin | 2 /5 /25 ml | L00683 |
| Ni-IDA Resin FF Prepacked Column | Ni-IDA-charged FPLC-compatible resin in a prepacked column | >40 mg of histidine-tagged protein /ml settled resin | 2 /5 /25 ml | L00684 | |
| GST fusion protein purification | Glutathione Resin | Glutathione | 35-45 mg GST-tagged protein (26kD) /ml settled resin | 10 ml | L00206 |
| GST Fusion Protein Purification Kit | 1 kit | L00207 | |||
| Purification of DYKDDDDK tagged protein | MonoRab™ Anti-DYKDDDDK Affinity Resin | MonoRab™ Anti-DYKDDDDK antibody | Approximately 1.5 mg DYKDDDDK-tagged protein/ml settled resin | 1 ml 5 ml 25 ml |
L00766 |
| Anti-DYKDDDDK G1 Affinity Resin | Anti-DYKDDDDK G1 antibody | Approximately 1 mg DYKDDDDK-tagged protein/ml settled resin | 1 ml 5 ml 10 ml 25 ml |
L00432 | |
| Purification of biotinylated protein, antibody, lectin etc. | Streptavidin Resin | Streptavidin | 120 nmol D-Binotin/ml settled resin | 5 ml | L00353 |
| Immobilization of sulfhydryl-containing peptides, proteins and other ligands for following purification | High-Affinity Iodoacetyl Resin | >9 mg Goat IgG/ml settled resin | 10 ml | L00403 | |
| 1 kit | L00404 | ||||
| High-Affinity Antibody Purification Kit | |||||
| Immunoprecipitation | Anti-His Affinity Resin | THE™ anti-His monoclonal antibody | >0.8 mg of histidine-tagged protein /ml settled resin | 1/5 ml | L00439 |
| Chicken IgY Precipitating Resin | Goat Anti-Chicken IgY | Immunoprecipitation of chicken IgY or the enrichment of chicken primary IgY‐protein complexes. | 1 ml | L00405 | |
| Anti-DYKDDDDK IP Resin | Mouse anti-DYKDDDDK monoclonal antibody | Immunoprecipitation of DYKDDDDK-tagged protein or DYKDDDDK-tagged protein-target protein complex. | 1 ml | L00425 |
주문정보
Protein A, G and L Resins
| 제품번호 | 품명 | 규격 | 제조사 | 비고 |
| L00210 | Protein A Resin | 5 ml | GenScript | |
| L00433-55 | Monofinity A Resin Prepacked Column 5mlx5 | 5ml prepacked x 5 | GenScript | |
| L00433-51 | Monofinity A Resin Prepacked Column 5mlx1 | 5ml prepacked x 1 | GenScript | |
| L00433-5 | Monofinity A Resin | 5 L | GenScript | |
| L00433-25 | Monofinity A Resin | 25 ml | GenScript | |
| L00433-200 | Monofinity A Resin | 200 ml | GenScript | |
| L00433-15 | Monofinity A Resin Prepacked Column 1mlx5 | 1 ml prepacked x 5 | GenScript | |
| L00433-11 | Monofinity A Resin Prepacked Column 1mlx1 | 1 ml prepacked x 1 | GenScript | |
| L00433-10 | Monofinity A Resin | 10 L | GenScript | |
| L00433-1 | Monofinity A Resin | 1 L | GenScript | |
| L00464-5 | Protein A Resin FF | 5 ml | GenScript | |
| L00209 | Protein G Resin | 5 ml | GenScript | |
| L00239 | Protein L Resin | 2.5 ml | GenScript | |
| L00664-5 | Protein G Resin FF | 5 ml | GenScript | |
| L00664-25 | Protein G Resin FF | 25 ml | GenScript | |
| L00664-100 | Protein G Resin FF | 100 ml | GenScript | |
| L00680-55 | Protein A Resin FF Prepacked Column | 5 ml x 5 | GenScript | |
| L00680-51 | Protein A Resin FF Prepacked Column | 5 ml x 1 | GenScript | |
| L00680-12 | Protein A Resin FF Prepacked Column | 1 ml x 2 | GenScript | |
| L00681-55 | Protein G Resin FF Prepacked Column | 5 ml x 5 | GenScript | |
| L00681-51 | Protein G Resin FF Prepacked Column | 5 ml x 1 | GenScript | |
| L00681-12 | Protein G Resin FF Prepacked Column | 1 ml x 2 | GenScript | |
| L00430 | Protein A ELISA Kit | 1 kit | GenScript |
Tagged Protein Purification Resins
Surface Activated Resins
| 제품번호 | 품명 | 규격 | 제조사 | 비고 |
| L00403-500 | High-Affinity Iodoacetyl Resin | 500 ml | GenScript | |
| L00403-200 | High-Affinity Iodoacetyl Resin | 200 ml | GenScript | |
| L00403 | High-Affinity Iodoacetyl Resin | 10 ml | GenScript | |
| L00404 | High-Affinity Antibody Purification Kit | 1 kit | GenScript |
Resins for Immunoprecipitation
| 제품번호 | 품명 | 규격 | 제조사 | 비고 |
| L00439-5 | Anti-His Affinity Resin | 5 ml | GenScript | |
| L00439-1 | Anti-His Affinity Resin | 1 ml | GenScript | |
| L00425 | Anti-DYKDDDDK IP Resin | 1 ml | GenScript |
EZarose Beads
| 제품번호 | 품명 | 규격 | 제조사 | 비고 |
| L00462-500 | EZarose™ 4B | 500 ml | GenScript | |
| L00462-5 | EZarose™ 4B | 5 L | GenScript | |
| L00462-100 | EZarose™ 4B | 100 ml | GenScript | |
| L00462-10 | EZarose™ 4B | 10 L | GenScript | |
| L00462-1 | EZarose™ 4B | 1 L | GenScript | |
| L00463-500 | EZarose™ 4FF | 500 ml | GenScript | |
| L00463-5 | EZarose™ 4FF | 5 L | GenScript | |
| L00463-100 | EZarose™ 4FF | 100 ml | GenScript | |
| L00463-10 | EZarose™ 4FF | 10 L | GenScript | |
| L00463-1 | EZarose™ 4FF | 1 L | GenScript |
Customer Citations
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Zilio N., et al. A novel histone deacetylase complex in the control of transcription and genome stability. Mol Cell Biol. 2014 Sep;15;34(18):3500-14.
Zhang H., et al. Longitudinally extensive NMO spinal cord pathology produced by passive transfer of NMO-IgG in mice lacking complement inhibitor CD59. J Autoimmun. 2014 Sep;53:67-77.
Ma L., et al. Identification of small ubiquitin-like modifier substrates with diverse functions using the Xenopus egg extract system. Mol Cell Proteomics. 2014 Jul;13(7):1659-75.
Liu W., et al. In a nongenomic action, steroid hormone 20-hydroxyecdysone induces phosphorylation of cyclin-dependent kinase 10 to promote gene transcription. Endocrinology. 2014 May;155(5):1738-50.
Asavapanumas N., et al. Unique neuromyelitis optica pathology produced in na?ve rats by intracerebral administration of NMO-IgG. Acta Neuropathol. 2014 Apr;127(4):539-51.
Lin H., et al. A novel human Fab antibody for Trop2 inhibits breast cancer growth in vitro and in vivo. Int J Cancer. 2014 Mar 1;134(5):1239-49.
Zhang D., et al. The acid phosphatase-encoding gene GmACP1 contributes to soybean tolerance to low-phosphorus stress. PLoS Genet. 2014 Jan;10(1):e1004061.
Lan JF., et al. Prohibitin Interacts with envelope proteins of white spot syndrome virus and prevents infection in the red swamp crayfish, Procambarus clarkii. J Virol. 2013 Dec;87(23):12756-65.
Chen Y., et al. Structure-function analysis of severe acute respiratory syndrome coronavirus RNA cap guanine-N7-methyltransferase. J Virol. 2013 Jun;87(11):6296-305.
Tubon TC Jr, et al. dCREB2-mediated enhancement of memory formation. J Neurosci. 2013 Apr 24;33(17):7475-87.
de Oliveira S, et al. Cxcl8 (IL-8) mediates neutrophil recruitment and behavior in the zebrafish inflammatory response. J Immunol. 2013 Apr 15;190(8):4349-59.
Tian M., et al. The combined use of photoaffinity labeling and surface plasmon resonance-based technology identifies multiple salicylic acid-binding proteins. Plant J. 2012 Dec;72(6):1027-38.
Lei Y., et al. The mitochondrial proteins NLRX1 and TUFM form a complex that regulates type I interferon and autophagy. Immunity. 2012 Jun 29;36(6):933-46.
Worzfeld T., et al. ErbB-2 signals through Plexin-B1 to promote breast cancer metastasis. J Clin Invest. 2012 Apr 2;122(4):1296-305.
Rouet R., et al. Expression of high-affinity human antibody fragments in bacteria. Nat Protoc. 2012 Feb 2;7(2):364-73.
Hu J., et al. The Bphi008a Gene Interacts with the Ethylene Pathway and Transcriptionally Regulates MAPK Genes in the Response of Rice to Brown Planthopper Feeding. Plant Physiol. 2011 Jun;156(2):856-72.
Staron M., et al. Heat-shock protein gp96/grp94 is an essential chaperone for the platelet glycoprotein Ib-IX-V complex. Blood. 2011 Jun 30;117(26):7136-44.
Newell PD., et al. A c-di-GMP effector system controls cell adhesion by inside-out signaling and surface protein cleavage. PLoS Biol. 2011 Feb 1;9(2):e1000587.
Document
| 제품 자료 | Purification resin flyer.pdf | Download Size: 1.71MB |
| 제품메뉴얼 |