Project Description

Protein Purification Resins

제품 소개

단백질 정제는 관심있는 단백질의 기능, 구조 및 상호 작용을 이해하는데 매우 중요한 첫 단계입니다.
여러 정제 방법 중 affinity chromatography는 표적 단백질에 대해 높은 선택성, 고분해능 및 고용량을 제공할 수 있어 가장 강력한 정제 방법으로 활용되고 있습니다.

GenScript는 batch/gravity purification을 위한 다양한 종류의 affinity purification resins을 제공하여 native sample로부터 단백질과 항체를 효율적이고 편리하게 분리할 수 있게 도와 주고 있습니다.

제품 특징

• 단백질 및 항체 정제를 위한 우수한 affinity chromatography media
• batch or gravity-flow operation 을 위한 빠르고 간단한 과정
• 비특이적 결합 확률이 매우 낮아 타겟에 대한 높은 결합 능력을 보임
• 다양한 샘플 종류 및 버퍼와 호환
• 여러 번 재사용 가능하여 비용 효율성 향상

Applications

• Antibody purification
• His and GST tagged protein purification
• Purification of streptavidin, avidin, or their conjugates
• Purification of biotinylated protein, antibody, lectin, etc

Selection guide

Application Product Ligand used Binding capacity Size Cat.No.
Antibody purification Protein A Resin Recombinant protein A >20 mg human IgG/ml settled resin 5 ml L00210
Protein A Resin FF Recombinant protein A on FPLC-compatible resin > 40 mg human IgG/ml settled resin 5 ml L00464
Protein G Resin Recombinant protein G >20 mg sheep IgG/ml settled resin 5 ml L00209
Protein G Resin FF Recombinant protein G on FPLC-compatible resin ≥ 20 mg human IgG/m settled resin 5/25/100 ml L00664
Protein L Resin Recombinant protein L >15 mg rabbit IgG/ml settled resin 2.5 ml L00239
Alkaline stable antibody purification resin Monofinity A Resin Recombinant alkaline tolerant protein A >30 mg human IgG/ml settled resin 25/200 ml
1/5/10 L
custom
L00433
Antibody purification (Prepacked Column) Monofinity A Resin Prepacked Column Recombinant alkaline tolerant protein A in a prepacked column >30 mg human IgG/ml settled resin 1/5/25 ml L00433
Protein A Resin FF Prepacked Column Recombinant alkaline tolerant protein A on FPLC-compatible resin in a prepacked column > 40 mg human IgG/ ml settled resin 2 /5 /25 ml L00680
Protein G Resin FF Prepacked Column Recombinant protein G on FPLC-compatible resin in a prepacked column ≥ 20 mg human IgG/ml settled resin 2 /5 /25 ml L00681
His-tagged protein purification High Affinity Ni-charged Resin Ni-NTA-charged resin 45-60 mg 6xHis-tagged protein (27 kD) /ml settled resin 10 ml
25 ml
500 ml
L00223
Ni Resin FF Ni-IDA-charged FPLC-compatible resin >40 mg 6xhistidine-tagged protein /ml settled resin 5 ml
25 ml
L00465
High Affinity Ni-Charged Resin FF Ni-NTA-charged FPLC-compatible resin >45 mg of histidine-tagged protein /ml settled resin 5/25/100 ml L00666
His-tagged protein purification (Prepacked Column) High Affinity Ni-Charged Resin FF Prepacked Column Ni-NTA-charged FPLC-compatible resin in a prepacked column >45 mg of histidine-tagged protein /ml settled resin 2 /5 /25 ml L00683
Ni-IDA Resin FF Prepacked Column Ni-IDA-charged FPLC-compatible resin in a prepacked column >40 mg of histidine-tagged protein /ml settled resin 2 /5 /25 ml L00684
GST fusion protein purification Glutathione Resin Glutathione 35-45 mg GST-tagged protein (26kD) /ml settled resin 10 ml L00206
GST Fusion Protein Purification Kit 1 kit L00207
Purification of DYKDDDDK tagged protein MonoRab™ Anti-DYKDDDDK Affinity Resin MonoRab™ Anti-DYKDDDDK antibody Approximately 1.5 mg DYKDDDDK-tagged protein/ml settled resin 1 ml
5 ml
25 ml
L00766
Anti-DYKDDDDK G1 Affinity Resin Anti-DYKDDDDK G1 antibody Approximately 1 mg DYKDDDDK-tagged protein/ml settled resin 1 ml
5 ml
10 ml
25 ml
L00432
Purification of biotinylated protein, antibody, lectin etc. Streptavidin Resin Streptavidin 120 nmol D-Binotin/ml settled resin 5 ml L00353
Immobilization of sulfhydryl-containing peptides, proteins and other ligands for following purification High-Affinity Iodoacetyl Resin >9 mg Goat IgG/ml settled resin 10 ml L00403
1 kit L00404
High-Affinity Antibody Purification Kit
Immunoprecipitation Anti-His Affinity Resin THE™ anti-His monoclonal antibody >0.8 mg of histidine-tagged protein /ml settled resin 1/5 ml L00439
Chicken IgY Precipitating Resin Goat Anti-Chicken IgY Immunoprecipitation of chicken IgY or the enrichment of chicken primary IgY‐protein complexes. 1 ml L00405
Anti-DYKDDDDK IP Resin Mouse anti-DYKDDDDK monoclonal antibody Immunoprecipitation of DYKDDDDK-tagged protein or DYKDDDDK-tagged protein-target protein complex. 1 ml L00425

주문정보

Protein A, G and L Resins

​제품번호 품명 규격 제조사 비고
L00210 Protein A Resin 5 ml GenScript
L00433-55 Monofinity A Resin Prepacked Column 5mlx5 5ml prepacked x 5
L00433-51 Monofinity A Resin Prepacked Column 5mlx1 5ml prepacked x 1
L00433-5 Monofinity A Resin 5 L
L00433-25 Monofinity A Resin 25 ml
L00433-200 Monofinity A Resin 200 ml
L00433-15 Monofinity A Resin Prepacked Column 1mlx5 1 ml prepacked x 5
L00433-11 Monofinity A Resin Prepacked Column 1mlx1 1 ml prepacked x 1
L00433-10 Monofinity A Resin 10 L
L00433-1 Monofinity A Resin 1 L
L00464-5 Protein A Resin FF 5 ml
L00209 Protein G Resin 5 ml
L00239 Protein L Resin 2.5 ml
L00664-5 Protein G Resin FF 5 ml
L00664-25 Protein G Resin FF 25 ml
L00664-100 Protein G Resin FF 100 ml
L00680-55 Protein A Resin FF Prepacked Column 5 ml x 5
L00680-51 Protein A Resin FF Prepacked Column 5 ml x 1
L00680-12 Protein A Resin FF Prepacked Column 1 ml x 2
L00681-55 Protein G Resin FF Prepacked Column  5 ml x 5
L00681-51 Protein G Resin FF Prepacked Column  5 ml x 1
L00681-12 Protein G Resin FF Prepacked Column  1 ml x 2
L00430 Protein A ELISA Kit 1 kit


Tagged Protein Purification Resins

​ 제품번호 품명 규격 제조사 비고
L00223-500 High Affinity Ni-Charged Resin 500 ml GenScript
L00223-25 High Affinity Ni-Charged Resin 25 ml
L00223-10 High Affinity Ni-Charged Resin 10 ml
L00206 Glutathione Resin 10 ml
L00353 Streptavidin Resin 5 ml
L00766-5 MonoRab™ Anti-DYKDDDDK Affinity Resin 5 ml
L00766-25 MonoRab™ Anti-DYKDDDDK Affinity Resin 25 ml
L00766-1 MonoRab™ Anti-DYKDDDDK Affinity Resin 1 ml
L00432-5 Anti-DYKDDDDK G1 Affinity Resin 5 ml
L00432-25 Anti-DYKDDDDK G1 Affinity Resin 25 ml
L00432-10 Anti-DYKDDDDK G1 Affinity Resin 10 ml
L00432-1 Anti-DYKDDDDK G1 Affinity Resin 1 ml
L00207 GST Fusion Protein Purification Kit 1 kit
L00465-5 Ni Resin FF 5 ml
L00465-25 Ni Resin FF 25 ml
L00666-5 High Affinity Ni-Charged Resin FF  5 ml
L00666-25 High Affinity Ni-Charged Resin FF  25 ml
L00666-100 High Affinity Ni-Charged Resin FF  100 ml
L00683-55 High Affinity Ni-Charged Resin FF Prepacked Column 5 ml x 5
L00683-51 High Affinity Ni-Charged Resin FF Prepacked Column 5 ml x 1
L00683-12 High Affinity Ni-Charged Resin FF Prepacked Column 1 ml x 2
L00684-55 Ni-IDA Resin FF Prepacked Column  5 ml x 5
L00684-51 Ni-IDA Resin FF Prepacked Column  5 ml x 1
L00684-12 Ni-IDA Resin FF Prepacked Column  1 ml x 2


Surface Activated Resin
s

​ 제품번호 품명 규격 제조사 비고
L00403-500 High-Affinity Iodoacetyl Resin 500 ml GenScript
L00403-200 High-Affinity Iodoacetyl Resin 200 ml
L00403 High-Affinity Iodoacetyl Resin 10 ml
L00404 High-Affinity Antibody Purification Kit 1 kit


Resins for Immunoprecipitation

​ 제품번호 품명 규격 제조사 비고
L00439-5 Anti-His Affinity Resin 5 ml GenScript
L00439-1 Anti-His Affinity Resin 1 ml
L00425 Anti-DYKDDDDK IP Resin 1 ml


EZarose Beads

​ 제품번호 품명 규격 제조사 비고
L00462-500 EZarose™ 4B 500 ml GenScript
L00462-5 EZarose™ 4B 5 L
L00462-100 EZarose™ 4B 100 ml
L00462-10 EZarose™ 4B 10 L
L00462-1 EZarose™ 4B 1 L
L00463-500 EZarose™ 4FF 500 ml
L00463-5 EZarose™ 4FF 5 L
L00463-100 EZarose™ 4FF 100 ml
L00463-10 EZarose™ 4FF 10 L
L00463-1 EZarose™ 4FF 1 L

Customer Citations

Hwang JH., et al. Polyoma Small T Antigen Interacts with YES-Associated Protein (YAP) to Regulate Cell Survival and Differentiation. J Virol. 2014 Oct;88(20):12055-64.

Gow DJ., et al. Characterisation of a Novel Fc Conjugate of Macrophage Colony-stimulating Factor. Mol Ther. 2014 Sep;22(9):1580-92.

Zilio N., et al. A novel histone deacetylase complex in the control of transcription and genome stability. Mol Cell Biol. 2014 Sep;15;34(18):3500-14.

Zhang H., et al. Longitudinally extensive NMO spinal cord pathology produced by passive transfer of NMO-IgG in mice lacking complement inhibitor CD59. J Autoimmun. 2014 Sep;53:67-77.

Ma L., et al. Identification of small ubiquitin-like modifier substrates with diverse functions using the Xenopus egg extract system. Mol Cell Proteomics. 2014 Jul;13(7):1659-75.

Liu W., et al. In a nongenomic action, steroid hormone 20-hydroxyecdysone induces phosphorylation of cyclin-dependent kinase 10 to promote gene transcription. Endocrinology. 2014 May;155(5):1738-50.

Asavapanumas N., et al. Unique neuromyelitis optica pathology produced in na?ve rats by intracerebral administration of NMO-IgG. Acta Neuropathol. 2014 Apr;127(4):539-51.

Lin H., et al. A novel human Fab antibody for Trop2 inhibits breast cancer growth in vitro and in vivo. Int J Cancer. 2014 Mar 1;134(5):1239-49.
Zhang D., et al. The acid phosphatase-encoding gene GmACP1 contributes to soybean tolerance to low-phosphorus stress. PLoS Genet. 2014 Jan;10(1):e1004061.

Lan JF., et al. Prohibitin Interacts with envelope proteins of white spot syndrome virus and prevents infection in the red swamp crayfish, Procambarus clarkii. J Virol. 2013 Dec;87(23):12756-65.

Chen Y., et al. Structure-function analysis of severe acute respiratory syndrome coronavirus RNA cap guanine-N7-methyltransferase. J Virol. 2013 Jun;87(11):6296-305.

Tubon TC Jr, et al. dCREB2-mediated enhancement of memory formation. J Neurosci. 2013 Apr 24;33(17):7475-87.
de Oliveira S, et al. Cxcl8 (IL-8) mediates neutrophil recruitment and behavior in the zebrafish inflammatory response. J Immunol. 2013 Apr 15;190(8):4349-59.

Tian M., et al. The combined use of photoaffinity labeling and surface plasmon resonance-based technology identifies multiple salicylic acid-binding proteins. Plant J. 2012 Dec;72(6):1027-38.

Lei Y., et al. The mitochondrial proteins NLRX1 and TUFM form a complex that regulates type I interferon and autophagy. Immunity. 2012 Jun 29;36(6):933-46.

Worzfeld T., et al. ErbB-2 signals through Plexin-B1 to promote breast cancer metastasis. J Clin Invest. 2012 Apr 2;122(4):1296-305.
Rouet R., et al. Expression of high-affinity human antibody fragments in bacteria. Nat Protoc. 2012 Feb 2;7(2):364-73.

Hu J., et al. The Bphi008a Gene Interacts with the Ethylene Pathway and Transcriptionally Regulates MAPK Genes in the Response of Rice to Brown Planthopper Feeding. Plant Physiol. 2011 Jun;156(2):856-72.

Staron M., et al. Heat-shock protein gp96/grp94 is an essential chaperone for the platelet glycoprotein Ib-IX-V complex. Blood. 2011 Jun 30;117(26):7136-44.

Newell PD., et al. A c-di-GMP effector system controls cell adhesion by inside-out signaling and surface protein cleavage. PLoS Biol. 2011 Feb 1;9(2):e1000587.

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