Project Description
PCRBIO Ultra Polymerase and Mix
제품 소개
PCRBIO Ultra Polymerase는 매우 어려운 template 및 long PCR 증폭에 적합하며 항체 매개 hot start 기술을 기반으로 Taq 효율을 높이기 위해 HS Taq DNA 중합효소를 변형하여 만든 제품입니다. Target gene에 대한 매우 높은 특이성을 가지고 있어 프라이머 dimer 형성 및 PCR 증폭을 억제하며 GC, AT 비율이 높은 복잡한 template 증폭에도 효과적입니다.
다른 DNA 중합효소에 비해 향상된 민감도와 특이성을 제공하며, 특히 Lambda DNA에서는 35 kb, human 게놈 DNA와 같은 복잡한 template에서 25 kb까지 증폭 가능 한 제품입니다.
제품특징
• primer dimer 및 비 특이적 증폭을 억제하는 hot-start 기술
• 5’→3’ exonuclease activity
• Amplification size: < 35 kb
• A-tail: Yes
응용분야
• Next-generation sequencing
• Difficult PCR
• TA Cloning
• Low copy template detection
• Multiplex PCR
보관온도
• -20℃
Figure 1. Long PCR products visualized on agarose gel
Amplification of 0.8kb, 1.0kb, 1.8kb, and 2.7kb fragments of the GAPDH gene, a 4.6kb fragment of the RBL15 gene, and a 5.9kb fragment of the MYH6 gene.
The starting template amount is 20ng (50ng for the 5.9kb fragment) of mouse genomic DNA and is diluted 2 to 5 fold.
Figure 2. GC rich products visualized on agarose gel
Amplification of 0.5kb (P1) and 0.6kb (P2) fragments of the ATXN2 gene with GC contents of 69% and 71%, respectively, using 20ng of mouse genomic DNA as a template and a range of annealing temperatures from 67°C to 60°C (B-E).
Figure 3. Room temperature stable for 4 weeks
PCRBIO Ultra Polymerase shows no change in activity after 14 days at 4°C, 25°C and 37°C, and after 28 days at 4°C and 25°C.
주문정보
| 제품번호 | 품명 | 규격 | 제조사 | 비교 |
| PB10.31-02 | PCRBIO Ultra Polymerase | 250 units | PCRbiosystems | [1 x 0.05ml 5 units/μl] & [2 x 1ml buffer] |
| PB10.31-10 | PCRBIO Ultra Polymerase | 1000 units | PCRbiosystems | [4 x 0.05ml 5 units/μl] & [8 x 1ml buffer] |
| PB10.32-01 | PCRBIO Ultra Mix | 80 x 50 μL Reactions | PCRbiosystems | 2 x 1ml |
| PB10.32-05 | PCRBIO Ultra Mix | 400 x 50 μL Reactions | PCRbiosystems | 10 x 1ml |
| PB10.33-01 | PCRBIO Ultra Mix Red | 80 x 50 μL Reactions | PCRbiosystems | 2 x 1ml |
| PB10.33-05 | PCRBIO Ultra Mix Red | 400 x 50 μL Reactions | PCRbiosystems | 10 x 1ml |
관련 논문
PCRBIO Ultra Mix
El-Attar, L.M.R., Mitchell, J.A., Brownlie, H.B., Priestnall, S.L. and Brownlie, J., 2015. Detection of nonprimate hepaciviruses in UK dogs. Virology, 484, pp.93-102.
Paclikova, H., 2016. Diverzita, evoluce a selekce u genů kódujících toll-like receptory TLR2 a TLR9 u čeledi Equidae (Doctoral dissertation, Masarykova univerzita, Přírodovědecká fakulta).
Document
| 제품 자료 | PCRBIO-Ultra-Polymerase-Product-Flyer.pdf | Upload date: 2021.07.02 |
| 제품 매뉴얼 | PB10.31-PCRBIO-Ultra-Polymerase-Manual.pdf PB10.32-PCRBIO-Ultra-Mix-Manual.pdf PB10.33-PCRBIO-Ultra-Mix-Red-Manual.pdf |
Upload date: 2021.07.02 |
| 제품 MSDS | PB10.31-PCRBIO-Ultra-Polymerase-MSDS.pdf PB10.32-PCRBIO-Ultra-Mix-MSDS.pdf PB10.33-PCRBIO-Ultra-Mix-Red-MSDS.pdf |
Upload date: 2021.07.02 |