Project Description

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PCRBIO HiFi Polymerase

제품 소개

PCRBIO HiFi Polymerase는 3’→5’ exonuclease (proofreading) activity를 갖고 있어 error rate이 낮아 정확한 PCR 증폭이 가능합니다.
Pfu에 의해 생성된 PCR 산물은 blunt-end form 이므로 일반적으로 TA cloning이 불가능합니다.
GC, AT 비율이 높은 복잡한 template의 증폭에도 효과적이며, 수율을 증가 시키고 cycling 시간을 단축시킬 수 있습니다.

제품특징

• Taq DNA polymerase 보다 50배 높은 정확도
• Amplification size: < 10 kb
• 3’→5’ exonuclease (proofreading) 활성
• A-tail: No

응용분야

• High fidelity PCR
• Fast PCR – 35 cycles of 5kb amplicon in under 1.5 hours
• Blunt-end cloning
• Site directed mutagenesis
• Nest generation re-sequencing

보관온도

• -20℃, stable for 4weeks up to at 37℃

Figure 1. Shows amplification of a 5kb amplicon from GAPDH derived from purified human genomic DNA. A 2 fold template dilution series was made from a starting concentration of 100 nanograms of DNA. 25 cycles of 30 seconds denaturation, 30 seconds annealing, and 75 seconds extension were completed in 1 hour. The first row shows PCRBIO HiFi Polymerase, the second row an equivalent product from Finnzymes, and the 3rd-row standard Pfu.

Figure 2. Shows no change in activity is detected in PCRBIO HiFi Polymerase after 28 days at 4˚C, 25˚C and 37˚C.

Figure 3. Shows PCRBIO HiFi Polymerase amplifying a 60% GC 1kb fragment of human GAPDH from genomic DNA. The template is diluted 2 fold over 8 orders of magnitude, starting from 100 nanograms. The first row shows PCRBIO HiFi Polymerase, the second row shows Invitrogens Pfx enzyme, and the 3rd-row Finnzymes Phusion enzyme.

주문정보

​제품번호 품명 규격 제조사 비교
PB10.41-02 PCRBIO HiFi Polymerase 200 units PCRbiosystems [1 x 0.1ml 2 units/μl] & [3 x 1ml buffer]
PB10.41-10 PCRBIO HiFi Polymerase 1,000 units PCRbiosystems [5 x 0.1ml 2 units/μl] & [15 x 1ml buffer]

관련 논문

PCRBIO HiFi Polymerase

Bang-Andreasen, T., Nielsen, J.T., Voriskova, J., Heise, J., Rønn, R., Kjøller, R., Hansen, H.C. and Jacobsen, C.S., 2017. Wood Ash Induced pH Changes Strongly Affect Soil Bacterial Numbers and Community Composition. Frontiers in Microbiology, 8.

Bech, T.B., Rosenbom, A.E., Sørensen, S.R. and Jacobsen, C.S., 2017. Conservative tracer bromide inhibits pesticide mineralisation in soil. Environmental Pollution, 222, pp.404-411.

Cameron, K.A., Stibal, M., Zarsky, J.D., Gözdereliler, E., Schostag, M. and Jacobsen, C.S., 2016. Supraglacial bacterial community structures vary across the Greenland ice sheet. FEMS microbiology ecology, 92(2).

Gilman, F.R., 2015. Microbial responses and contributions to climate change in Greenland. University of Montana, Missoula, Montana, USA.

Herrmann, B., 2017. Autökologische und molekularbiologische Untersuchungen zur Charakterisierung der Kleidermotte Tineola bisselliella (Lepidoptera) als Referenzorganismus (Doctoral dissertation, Freie Universität Berlin). Supervisors: Dr. Rüdiger Plarre; Prof. Dr. Rupert Mutzel

Kirkegaard, R.H., McIlroy, S.J., Kristensen, J.M., Nierychlo, M., Karst, S.M., Dueholm, M.S., Albertsen, M. and Nielsen, P.H., 2017. Identifying the abundant and active microorganisms common to full scale anaerobic digesters. Biorxiv, p.104620.

Martín Ramírez, R.M., 2014. Estudio de expresión de la familia génica sirtuínas en células de la granulosa humana, y su relación con la calidad oocitaria en técnicas de fecundación in vitro. Universidad de La Laguna, Santa Cruz de Tenerife, Spain.

Pickering, H., 2017. Identification of Chlamydia trachomatis Immune Targets through Immunological and Population-Genomic Screens and Elucidation of Potential Roles in Bacterial Pathogenesis (Doctoral dissertation, London School of Hygiene & Tropical Medicine). Supervisors: Martin Holland; Richard Hayward.

Repin, R.A.M., Mutalib, S.A., Shahimi, S., Khalid, R.M., Ayob, M.K., Bakar, M.F.A. and Isa, M.N.M., 2016, November. Bioinformatics analysis and detection of gelatinase encoded gene in Lysinibacillussphaericus. In AIP Conference Proceedings (Vol. 1784, No. 1, p. 030044). AIP Publishing.

Stankiewicz, E., 2017. Identification of the genetic alterations in prostate cancer metastases (Doctoral dissertation, Queen Mary University of London). Supervisor: Dr Yong-Jie Lu and Prof D. Berney

Document

제품 자료 PCRBIO-HiFi-Polymerase-Flyer Download Size : 726kB
제품 MSDS PCRBIO-HiFi-Polymerase-MSDS Download Size : 288kB
제품 매뉴얼 PCRBIO-HiFi-Polymerase-Manual Download Size : 44.4kB