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PCRBIO Taq DNA Polymerase & Mixes

제품 소개

PCRBIO Taq DNA Polymerase는 강력하고, 합리적인 가격의 enzyme으로 genotyping, screening 및 library construction을 포함한 일상적 PCR 어플리케이션에 이상적인 제품입니다.
5’→3’ exonuclease 활성을 감소시켜 비특이적 증폭산물의 형성을 효과적으로 저해합니다.
효소의 촉매반응부위의 유전자 조작을 통하여 GC 비율, AT 비율이 높은 복잡한 template의 증폭에도 효과적입니다.

제품특징

• Amplification size: < 6 kb
• 오차율: 2.0 x 105
• 5’→3’ exonuclease activity
• 증폭 된 DNA의 3’말단에는 A-tail이 형성
• 안정성: 25℃ – 37℃에서 4주간 안정

응용분야

• Standard and fast PCR
• Routine PCR
• TA cloning
• Methylated DNA

보관온도

• -20℃

taq_pol_2

Figure 1. Shows amplification of a 1.2kb fragment of 60% GC GADPH from human genomic DNA in a 3 fold dilution from left to right.
Starting concentration is 200ng of DNA and is diluted to 0.7pg in the 7th dilution. PCRBIO Taq DNA Polymerase is able to amplify lower concentration template DNA compared with competitors P and I (rows 2 and 3).

taq_pol_3

Figure 2. No change in activity is detected in PCRBIO Taq DNA Polymerase after 28 days at 4°C, 25°C, and 37°C.

주문정보

제품번호 품명 규격 제조사 비고
PB10.11-05 PCRBIO Taq DNA Polymerase 500 units PCRBiosystems [1 x 100ul] & [4 x 1mL buffer]
PB10.11-20 PCRBIO Taq DNA Polymerase 2,000 units PCRBiosystems [4 x 100ul] & [16 x 1mL buffer]
PB10.12-02 PCRBIO Taq Mix 200 rxns PCRBiosystems 5 x 1mL
PB10.12-10 PCRBIO Taq Mix 1,000 rxns PCRBiosystems 25 x 1m
PB10.13-02 PCRBIO Taq Mix Red 200 rxns PCRBiosystems 5 x 1mL
PB10.13-10 PCRBIO Taq Mix Red 1,000 rxns PCRBiosystems 25 x 1mL
PB10.13-96 PCRBIO Taq PreMix 96 rxns PCRBiosystems 96 tubes
PB10.13-480 PCRBIO Taq PreMix 480 rxns PCRBiosystems 96 tubes x 5

관련 논문

PCRBIO Taq DNA Polymerase

Adilah-Amrannudin, N., Hamsidi, M., Ismail, N.A., Ismail, R., Dom, N.C., Ahmad, A.H., Mastuki, M.F., Basri, T.S.A.T.A., Khalid, A., Muslim, M., Daud, N.A.A. and Camalxaman, S.N., 2016. Genetic Polymorphism of Aedes albopictus Population Inferred From ND5 Gene Variabilities In Subang Jaya, Malaysia. Journal of the American Mosquito Control Association32(4), pp.265-272.

Koukidou, M., Alphey, L. and Warner, S., Oxitec Limited, 2015. Gene expression system. U.S. Patent Application 15/313,922.

Li, J., Zheng, Y., Xu, H., Xi, X., Hou, Q., Feng, S., Wuri, L., Bian, Y., Yu, Z., Kwok, L.Y., Sun, Z. and Sun, T. 2017. Bacterial microbiota of Kazakhstan cheese revealed by single molecule real time (SMRT) sequencing and its comparison with Belgian, Kalmykian and Italian artisanal cheeses. BMC microbiology17(1), p.13.

Maceda-Veiga, A., Webster, G., Canals, O., Salvadó, H., Weightman, A.J. and Cable, J., 2015. Chronic effects of temperature and nitrate pollution on Daphnia magna: Is this cladoceran suitable for widespread use as a tertiary treatment?. Water research83, pp.141-152.

Mitková, B., Hrazdilová, K., D’Amico, G., Duscher, G.G., Suchentrunk, F., Forejtek, P., Gherman, C.M., Matei, I.A., Ionică, A.M., Daskalaki, A.A., Mihalca, A.D., Votypka, J., Hulva, P. and Modry, D., 2017. Eurasian golden jackal as host of canine vector-borne protists. Parasites & vectors10(1), p.183.

Olivier, M.F., 2016. Temperature and salinity controls on methanogenesis in an artificial freshwater lake (Cardiff Bay, Wales) (Doctoral dissertation, Cardiff University). Szmolka, A., Szabó, M., Kiss, J., Pászti, J., Adrián, E., Olasz, F. and Nagy, B., 2017. Molecular epidemiology of the endemic multiresistance plasmid pSI54/04 of Salmonella Infantis in broiler and human population in Hungary. Food Microbiology.

Williams, A.S., 2015. The dynamic interaction between microbial biodiversity, biogeochemical activity and sedimentary geomorphology in the Severn Estuary (Doctoral dissertation, Cardiff University)


PCRBIO Taq Mix

Kang, J.T., Cho, B., Ryu, J., Ray, C., Lee, E.J., Yun, Y.J., Ahn, S., Lee, J., Ji, D.Y., Jue, N. and Clark-Deener, S., 2016. Biallelic modification of IL2RG leads to severe combined immunodeficiency in pigs. Reproductive Biology and Endocrinology14(1), p.74.

Kang, J.T., Ryu, J., Cho, B., Lee, E.J., Yun, Y.J., Ahn, S., Lee, J., Ji, D.Y., Lee, K. and Park, K.W., 2016. Generation of RUNX3 knockout pigs using CRISPR/Cas9‐mediated gene targeting. Reproduction in Domestic Animals51(6), pp.970-978.

Paclikova, H., 2016. Diverzita, evoluce a selekce u genů kódujících toll-like receptory TLR2 a TLR9 u čeledi Equidae (Doctoral dissertation, Masarykova univerzita, Přírodovědecká fakulta, Czechia).


PCRBIO Taq Mix Red

Gallusová, M., Jirsová, D., Mihalca, A.D., Gherman, C.M., D’Amico, G., Qablan, M.A. and Modrý, D., 2016. Cytauxzoon infections in wild felids from Carpathian-Danubian-Pontic space: further evidence for a different Cytauxzoon species in European felids. The Journal of parasitology102(3), pp.377-380.

Lee, S.Y. and Mohamed, R., 2016. Rediscovery of Aquilaria rostrata (Thymelaeaceae), a species thought to be extinct, and notes on Aquilaria conservation in Peninsular Malaysia. BlumeaBiodiversity, Evolution and Biogeography of Plants61(1), pp.13-19.

Lee, S.Y., Mohamed, R., Faridah-Hanum, I. and Lamasudin, D.U., 2017. Utilization of the internal transcribed spacer (ITS) DNA sequence to trace the geographical sources of Aquilaria malaccensis Lam. populations. Plant Genetic Resources, pp.1-9.

Lee, S.Y., Ng, W.L., Mahat, M.N., Nazre, M. and Mohamed, R., 2016. DNA barcoding of the endangered Aquilaria (Thymelaeaceae) and its application in species authentication of agarwood products traded in the market. PloS one11(4), p.e0154631.

Mitková, B., Hrazdilová, K., Steinbauer, V., D’Amico, G., Mihalca, A.D. and Modrý, D., 2016. Autochthonous Hepatozoon infection in hunting dogs and foxes from the Czech Republic. Parasitology research115(11), pp.4167-4171.

Sawei, J. and Sani, N.A., 2016, November. Prevalence, isolation and characterization of Bacillus cereus strains from rice of local cultivators of Sabah, Sarawak, and Peninsular Malaysia. In AIP Conference Proceedings (Vol. 1784, No. 1, p. 030029). AIP Publishing.

Shetty, S.M., Shah, M.U.M., Makale, K., Mohd-Yusuf, Y., Khalid, N. and Othman, R.Y., 2016. Complete chloroplast genome sequence of corroborates structural heterogeneity of inverted repeats in wild progenitors of cultivated bananas and plantains. The plant genome9(2).

Sváb, D., Bálint, B., Maróti, G. and Tóth, I., 2015. A novel transducible chimeric phage from Escherichia coli O157: H7 Sakai strain encoding Stx1 production. Infection, Genetics and Evolution29, pp.42-47.

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