Project Description
PCRBIO Taq DNA Polymerase & Mixes
제품 소개
CRBIO Taq DNA Polymerase는 합리적인 가격의 PCR enzyme으로 genotying, screening 및 library construciton을 포함한 기본적인 PCR application에 추천하는 제품입니다.
5’→3’ exonuclease 활성을 감소시켜 비특이적 증폭산물의 형성을 효과적으로 저해하는 특징을 가지며,
효소의 촉매반응부위의 유전자 조작을 통하여 GC 비율, AT 비율이 높은 복잡한 template의 증폭에도 효과적입니다.
제품특징
• Amplification size: < 6 kb
• 오차율: 2.0 x 105
• 5’→3’ exonuclease activity
• 증폭 된 DNA의 3’말단에는 A-tail이 형성
• 안정성: 25℃ – 37℃에서 4주간 안정
응용분야
• Standard and fast PCR
• Routine PCR
• TA cloning
• Methylated DNA
보관온도
• -20℃

Figure 1. Shows amplification of a 1.2kb fragment of 60% GC GADPH from human genomic DNA in a 3 fold dilution from left to right.
Starting concentration is 200ng of DNA and is diluted to 0.7pg in the 7th dilution. PCRBIO Taq DNA Polymerase is able to amplify lower concentration template DNA compared with competitors P and I (rows 2 and 3).

Figure 2. No change in activity is detected in PCRBIO Taq DNA Polymerase after 28 days at 4°C, 25°C, and 37°C.
주문정보
제품번호 | 품명 | 규격 | 제조사 | 비고 |
PB10.11-05 | PCRBIO Taq DNA Polymerase | 500 units | PCRBiosystems | [1 x 100ul] & [4 x 1mL buffer] |
PB10.11-20 | PCRBIO Taq DNA Polymerase | 2,000 units | PCRBiosystems | [4 x 100ul] & [16 x 1mL buffer] |
PB10.12-02 | PCRBIO Taq Mix | 200 x 50 μL Reactions | PCRBiosystems | 5 x 1mL |
PB10.12-10 | PCRBIO Taq Mix | 1000 x 50 μL Reactions | PCRBiosystems | 25 x 1m |
PB10.13-02 | PCRBIO Taq Mix Red | 200 x 50 μL Reactions | PCRBiosystems | 5 x 1mL |
PB10.13-10 | PCRBIO Taq Mix Red | 1000 x 50 μL Reactions | PCRBiosystems | 25 x 1mL |
PB10.13-96 | PCRBIO Taq PreMix | 96 rxns | PCRBiosystems | 96 tubes |
PB10.13-480 | PCRBIO Taq PreMix | 480 rxns | PCRBiosystems | 96 tubes x 5 |
관련 논문
PCRBIO Taq DNA Polymerase
Adilah-Amrannudin, N., Hamsidi, M., Ismail, N.A., Ismail, R., Dom, N.C., Ahmad, A.H., Mastuki, M.F., Basri, T.S.A.T.A., Khalid, A., Muslim, M., Daud, N.A.A. and Camalxaman, S.N., 2016. Genetic Polymorphism of Aedes albopictus Population Inferred From ND5 Gene Variabilities In Subang Jaya, Malaysia. Journal of the American Mosquito Control Association, 32(4), pp.265-272.
Koukidou, M., Alphey, L. and Warner, S., Oxitec Limited, 2015. Gene expression system. U.S. Patent Application 15/313,922.
Li, J., Zheng, Y., Xu, H., Xi, X., Hou, Q., Feng, S., Wuri, L., Bian, Y., Yu, Z., Kwok, L.Y., Sun, Z. and Sun, T. 2017. Bacterial microbiota of Kazakhstan cheese revealed by single molecule real time (SMRT) sequencing and its comparison with Belgian, Kalmykian and Italian artisanal cheeses. BMC microbiology, 17(1), p.13.
Maceda-Veiga, A., Webster, G., Canals, O., Salvadó, H., Weightman, A.J. and Cable, J., 2015. Chronic effects of temperature and nitrate pollution on Daphnia magna: Is this cladoceran suitable for widespread use as a tertiary treatment?. Water research, 83, pp.141-152.
Mitková, B., Hrazdilová, K., D’Amico, G., Duscher, G.G., Suchentrunk, F., Forejtek, P., Gherman, C.M., Matei, I.A., Ionică, A.M., Daskalaki, A.A., Mihalca, A.D., Votypka, J., Hulva, P. and Modry, D., 2017. Eurasian golden jackal as host of canine vector-borne protists. Parasites & vectors, 10(1), p.183.
Olivier, M.F., 2016. Temperature and salinity controls on methanogenesis in an artificial freshwater lake (Cardiff Bay, Wales) (Doctoral dissertation, Cardiff University). Szmolka, A., Szabó, M., Kiss, J., Pászti, J., Adrián, E., Olasz, F. and Nagy, B., 2017. Molecular epidemiology of the endemic multiresistance plasmid pSI54/04 of Salmonella Infantis in broiler and human population in Hungary. Food Microbiology.
Williams, A.S., 2015. The dynamic interaction between microbial biodiversity, biogeochemical activity and sedimentary geomorphology in the Severn Estuary (Doctoral dissertation, Cardiff University)
PCRBIO Taq Mix
Kang, J.T., Cho, B., Ryu, J., Ray, C., Lee, E.J., Yun, Y.J., Ahn, S., Lee, J., Ji, D.Y., Jue, N. and Clark-Deener, S., 2016. Biallelic modification of IL2RG leads to severe combined immunodeficiency in pigs. Reproductive Biology and Endocrinology, 14(1), p.74.
Kang, J.T., Ryu, J., Cho, B., Lee, E.J., Yun, Y.J., Ahn, S., Lee, J., Ji, D.Y., Lee, K. and Park, K.W., 2016. Generation of RUNX3 knockout pigs using CRISPR/Cas9‐mediated gene targeting. Reproduction in Domestic Animals, 51(6), pp.970-978.
Paclikova, H., 2016. Diverzita, evoluce a selekce u genů kódujících toll-like receptory TLR2 a TLR9 u čeledi Equidae (Doctoral dissertation, Masarykova univerzita, Přírodovědecká fakulta, Czechia).
PCRBIO Taq Mix Red
Gallusová, M., Jirsová, D., Mihalca, A.D., Gherman, C.M., D’Amico, G., Qablan, M.A. and Modrý, D., 2016. Cytauxzoon infections in wild felids from Carpathian-Danubian-Pontic space: further evidence for a different Cytauxzoon species in European felids. The Journal of parasitology, 102(3), pp.377-380.
Lee, S.Y. and Mohamed, R., 2016. Rediscovery of Aquilaria rostrata (Thymelaeaceae), a species thought to be extinct, and notes on Aquilaria conservation in Peninsular Malaysia. BlumeaBiodiversity, Evolution and Biogeography of Plants, 61(1), pp.13-19.
Lee, S.Y., Mohamed, R., Faridah-Hanum, I. and Lamasudin, D.U., 2017. Utilization of the internal transcribed spacer (ITS) DNA sequence to trace the geographical sources of Aquilaria malaccensis Lam. populations. Plant Genetic Resources, pp.1-9.
Lee, S.Y., Ng, W.L., Mahat, M.N., Nazre, M. and Mohamed, R., 2016. DNA barcoding of the endangered Aquilaria (Thymelaeaceae) and its application in species authentication of agarwood products traded in the market. PloS one, 11(4), p.e0154631.
Mitková, B., Hrazdilová, K., Steinbauer, V., D’Amico, G., Mihalca, A.D. and Modrý, D., 2016. Autochthonous Hepatozoon infection in hunting dogs and foxes from the Czech Republic. Parasitology research, 115(11), pp.4167-4171.
Sawei, J. and Sani, N.A., 2016, November. Prevalence, isolation and characterization of Bacillus cereus strains from rice of local cultivators of Sabah, Sarawak, and Peninsular Malaysia. In AIP Conference Proceedings (Vol. 1784, No. 1, p. 030029). AIP Publishing.
Shetty, S.M., Shah, M.U.M., Makale, K., Mohd-Yusuf, Y., Khalid, N. and Othman, R.Y., 2016. Complete chloroplast genome sequence of corroborates structural heterogeneity of inverted repeats in wild progenitors of cultivated bananas and plantains. The plant genome, 9(2).
Sváb, D., Bálint, B., Maróti, G. and Tóth, I., 2015. A novel transducible chimeric phage from Escherichia coli O157: H7 Sakai strain encoding Stx1 production. Infection, Genetics and Evolution, 29, pp.42-47.
Document
제품 자료 | PCRBIO-Taq-DNA-Polymerase-Product-Flyer.pdf PCRBIO-Taq-Mix-Red-Product-Flyer.pdf |
Upload data: 2021.07.02 |
제품 메뉴얼 | PB10.11-PCRBIO-Taq-DNA-Polymerase.pdf PB10.12-PCRBIO-Taq-Mix.pdf PB10.13-PCRBIO-Taq-Mix-Red.pdf |
Upload data: 2021.07.02 |
제품 MSDS | PB10.11-PCRBIO-Taq-DNA-Polymerase-MSDS.pdf PB10.12-PCRBIO-Taq-Mix-MSDS.pdf PB10.13-PCRBIO-Taq-Mix-Red-MSDS.pdf |
Upload data: 2021.07.02 |