Project Description
PCRBIO HiFi Polymerase
제품 소개
PCRBIO HiFi Polymerase는 3’→5’ exonuclease (proofreading) activity를 갖고 있어 error rate이 낮아 정확한 PCR 증폭이 가능합니다.
Pfu에 의해 생성된 PCR 산물은 blunt-end form 이므로 일반적으로 TA cloning이 불가능합니다.
GC, AT 비율이 높은 복잡한 template의 증폭에도 효과적이며, 수율을 증가 시키고 cycling 시간을 단축시킬 수 있습니다.
제품특징
• Taq DNA polymerase 보다 50배 높은 정확도
• Amplification size: < 10 kb
• 3’→5’ exonuclease (proofreading) 활성
• A-tail: No
응용분야
• High fidelity PCR
• Fast PCR – 35 cycles of 5kb amplicon in under 1.5 hours
• blunt-end cloning
• Site-directed mutagenesis
• Nest generation re-sequencing
보관온도
• -20℃
Figure 1. Shows amplification of a 5kb amplicon from GAPDH derived from purified human genomic DNA. A 2 fold template dilution series was made from a starting concentration of 100 nanograms of DNA. 25 cycles of 30 seconds denaturation, 30 seconds annealing, and 75 seconds extension were completed in 1 hour. The first row shows PCRBIO HiFi Polymerase, the second row an equivalent product from Finnzymes, and the 3rd-row standard Pfu.
Figure 3. Shows PCRBIO HiFi Polymerase amplifying a 60% GC 1kb fragment of human GAPDH from genomic DNA. The template is diluted 2 fold over 8 orders of magnitude, starting from 100 nanograms. The first row shows PCRBIO HiFi Polymerase, the second row shows Invitrogens Pfx enzyme, and the 3rd-row Finnzymes Phusion enzyme.
주문정보
제품번호 | 품명 | 규격 | 제조사 | 비교 |
PB10.41-02 | PCRBIO HiFi Polymerase | 200 units | PCRbiosystems | [1 x 0.1ml 2 units/μl] & [3 x 1ml buffer] |
PB10.41-10 | PCRBIO HiFi Polymerase | 1,000 units | PCRbiosystems | [5 x 0.1ml 2 units/μl] & [15 x 1ml buffer] |
관련 논문
PCRBIO HiFi Polymerase
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Document
제품 자료 | PCRBIO-HiFi-Polymerase-Product-Flyer.pdf | Upload date: 2021.07.02 |
제품 매뉴얼 | PB10.41-PCRBIO-HiFi-Polymerase-Manual.pdf | Upload date: 2021.07.02 |
제품 MSDS | PB10.41-PCRBIO-HiFi-Polymerase-MSDS.pdf | Upload date: 2021.07.02 |