Project Description

PCR DIRECT Swab Extraction Reagent

제품 소개

PCR DIRECT Swab Extraction Reagent는 swab에서 바이러스 및 host 핵산(DNA 및 RNA)의 비활성화 및 추출을 위한 것입니다.

Arcis PCR DIRECT 시약은 신속한 용해, 핵산 보호 및 잠재적인 PCR inhibitors의 차단을 위해 독점적인 One-Step™ chemistry 을 사용합니다.
연구자는 1번의 피펫팅으로도 direct PCR/RT-qPCR에 적합한 높은 감도와 재현성을 가진 결과물을 빠르게 얻을 수  있습니다.

이 시약은 뭉친 면봉 (flocked swab) 또는 NP-OP 면봉을 포함한 다양한 유형에 적합합니다.
PCR DIRECT Extracting Transport Medium(ETM)과 함께 사용하면, 채취시점에 바이러스를 비활성화 하고 숙주 핵산(DNA 및 RNA)의 추출 안정성을 도모할 수 있습니다.

제품특징

• 워크플로우 단순화-digestion step 제거
• CDC 프로토콜과 동등한 민감도 제공
• 점액 용해 특성으로 실패 확률 감소
• 단일 시약으로 실험 진행
• 최소 비용으로 안전한 실험 보장
• High Sensitivity SARS-CoV-2 Testing

제품사양

UFL141 UFL145
Sample type Nasopharyngeal, Oropharyngeal, Nasal Swab Nasopharyngeal, Oropharyngeal, Nasal Swab
Sample input volume One dry swab One dry swab
Reaction time 1-minute hands-on 1-minute hands-on
No. of reactions 40 400
Storage Store at room temperature, do not freeze Store at room temperature, do not freeze
Shipping conditions Room temperature Room temperature

프로토콜

Standard Protocol

1. Collect the dry swab according to laboratory procedure, in a dry tube without transport media.
2. Add 300µl of PCR DIRECT® ER1 to a low binding microtube (1.5-2.0ml)
3. Add the swab to the microtube, squeeze and turn the swab against the microtube wall to extract as much as possible of the mucus, at least ten (10) times
4. Pipette mix at least ten (10) times
5. Heat sample for five (5) minutes at 70°C (Optional)
6. Sample is ready for downstream molecular analysis or processing

Note: Inadequate mixing, squeeze, and turns in steps 3 and 4 will result in lower sensitivity. Mixing by pipetting is preferred

Nucleic acid yield and processing efficiency may vary depending on the virus extracted.
Samples prepared with the PCR DIRECT ER1 should be used in the intended downstream processes within 4 hours of preparation or frozen if intended for use within 24 hours.
Freeze-thaw cycles after adding PCR DIRECT ER1 should be limited to 2 or fewer.
For long-term storage of RNA, we recommend the addition of an RNase inhibitor in the amount recommended by the manufacturer to ensure long-term stability.

PCR Direct Guide to Product Selection

Arcis PCR DIRECT® Product Swab Extraction Reagent Saliva Extraction Reagent Extracting Transport Medium
For use in the Lab O O
For use at the point of collection* O
Extract nucleic acids from dry samples. Avoid the need for media O O
Simplify protocol:
no incubation, no capture, and concentration save time and money
O O O
Reduce viscosity of samples, enable automation O O O
Achieve higher sensitivity compared to other direct methods O O O
Avoid guanidinium & hazardous waste O O O
Achieve greatest possible safety through earliest inactivation O
Sequesters RNases O O O
Sample Type Swab Saliva Swab/Saliva

*Apply specified volume to sample with dropper or pre-aliquot into a collection device.

주문정보

​ 제품번호 품명 규격 제조사 비고
UFL141 PCR DIRECT Swab Extraction Reagent (40 Preps) 40 rxns Arcis Biotechnology
UFL145 PCR DIRECT Swab Extraction Reagent (400 Preps) 400 runs Arcis Biotechnology

Document

제품 자료
제품 MSDS MSDS-PCR_DIRECT_Extraction_Reagent.pdf  Upload date: 2022.02.23
제품 매뉴얼 PCR-DIRECT-UFL141.pdf
PCR-DIRECT-UFL145.pdf
Upload date: 2022.02.23