Collect saliva samples according to laboratory procedure, in dry tubes without transport media.
For best results, we recommend freezing samples while in storage prior to processing.
1. Add 100 µl of sample to a 1.5ml low binding microtube
2. Add 231µl of PCR DIRECT ER1 to the sample with the same pipette, mix ten (10) times
3. Heat sample for five (5) minutes at 70°C (Optional)
4. Sample is ready for downstream molecular analysis or processing
Note: Inadequate mixing in step 2 will result in lower sensitivity. Mixing by pipetting is preferred
Nucleic acid yield and processing efficiency may vary depending on the virus extracted.
Samples prepared with the PCR DIRECT ER1 should be used in the intended downstream processes within 4 hours of preparation or frozen if intended for use within 24 hours.
Freeze-thaw cycles after adding PCR DIRECT ER1 should be limited to 2 or fewer.
For longer-term storage of RNA, we recommend the addition of an RNase inhibitor in the amount recommended by the manufacturer to ensure long-term stability.
PCR DIRECT Saliva Extraction Reagent is applied in the lab.
This protocol may be adjusted for larger sample volumes by scaling the volume of PCR DIRECT ER1 in proportion to the increase in the sample volume.
To combine the advantages of Saliva Extraction Reagent with viral inactivation and transport at point of collection, see PCR DIRECT Extracting Transport Medium