Project Description
Mouse Anti-Vimentin
제품소개
Vimentin(57kDa)은 주로 정상 중간엽세포에서 발견되는 세포골격계 제3형 중간필라멘트 단백질 (intermediate filament protein, IFP)입니다.
백혈구 및 혈관내피세포에 광범위하게 분포하고 있는 단백질로써 주로 세포 모양을 유지하고, 세포질의 무결성을 유지하며, 세포 골격계 상호 작용을 안정화시키는 역할을 합니다.
Vimentin은 생체 내에서 구조적 스트레스를 받을 때 미세소관 또는 actin filament networks에 없는 복원력을 세포에 제공함으로서 세포에 유연성을 부여합니다.
그러나 종종 잘 조절되는 발현 패턴에서 벗어나 발달 초기 단계에서 여러 다른 IFP와 함께 조직 특이적으로 발현되면서 세포의 이상을 초래하기도 합니다.
Monoclonal Anti-Vimentin Antibody는 immunoblotting, immunohistochemistry and immunoprecipitation과 같은 다양한 면역화학적 분석에 의해 생체 내에서 Vimentin의 위치를 파악하여 세포 무결성을 확인하는데 유용한 도구로 활용될 수 있습니다.
Related brand: Nordic, Exalpha, Gallus, MUbio, Biologo, ADG, ELTI, Scicons, Bii
Source
RV202 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.
Product
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Specificity
RV202 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal-derived tumors e.g. lymphoma, sarcoma, and melanoma.
Applications
RV202 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissues, and flow cytometry.
Optimal antibody dilutions should be determined by titration; the recommended range is 1:100 – 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:1000 for immunoblotting applications.
Storage
The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store them at or below -20°C.
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Applications
Figure 1. Immunohistochemistry on frozen section of swine colon showing positive staining in connective tissue cells and no reactivity in epithelial cells.
Figure 2. The western blotting result shows the specific reactivity of MUB1900P with the 57kDa protein band of vimentin in both the mouse (3T3 mouse fibroblasts; left lane) and human (normal human dermal fibroblasts; right lane) cell extracts.
Figure 3. Immunohistochemistry on frozen section of swine colon showing positive staining in connective tissue cells and no reactivity in epithelial cells. Nuclear staining with DAPI.
Figure 4. Immunohistochemistry on frozen section of swine colon showing positive staining in connective tissue cells and no reactivity in epithelial cells. Nuclear staning with DAPI.
Figure 5. Immunohistochemistry on frozen section of swine colon showing positive staining in connective tissue cells and no reactivity in epithelial cells. Nuclear staining with DAPI.
Figure 6. Immunohistochemistry on frozen section of zebra fish embryo.
Figure 7. Immunohistochemistry on frozen section of zebra fish embryo.
Figure 8. Immunohistochemistry on frozen section of zebra fish embryo.
Figure 9. Immunohistochemistry on a formalin-fixed, paraffin-embedded section of human placenta showing positive staining in connective tissue cells and no reactivity in epithelial cells.
Figure 10. Immunohistochemistry on a formalin-fixed, paraffin-embedded section of human small intestine showing positive staining in connective tissue cells and no reactivity in epithelial cells.
Figure 11. Immunohistochemistry on a formalin-fixed, paraffin-embedded section of human small intestine showing positive staining in connective tissue cells and no reactivity in epithelial cells.
Figure 12. Immunohistochemistry on a formalin-fixed, paraffin-embedded section of human spleen showing positive staining in connective tissue cells and lymphoid cells.
Figure 13. Immunohistochemistry on a formalin-fixed, paraffin-embedded section of human tonsillar lymphoma.
Figure 14. Immunohistochemistry on frozen section of swine colon showing positive staining in connective tissue cells and no reactivity in epithelial cells. Nuclear staining with DAPI.
주요 제품
| Code | Name | Product Type | Clone |
| MUB1900L2 | Mouse anti-Vimentin, conjugated with HRP | Primary Antibodies | RV202 |
| MUB1903P | Recombinant mouse anti-Vimentin | Monoclonal Antibody | RV204 |
| MUB1904P | Recombinant rabbit anti-Vimentin | Monoclonal Antibody | RV205 |
| MUB1900L1 | Mouse anti-Vimentin, conjugated with FITC | Primary Antibodies | RV202 |
| MUB1900L1-CE/IVD | Mouse anti-Vimentin, conjugated with FITC | Primary Antibodies | RV202 |
| MUB1900P-CE/IVD | Mouse anti-Vimentin | Monoclonal Antibody | RV202 |
| MUB1900P | Mouse anti-Vimentin | Primary Antibodies | RV202 |
| MUB1901P | Mouse anti-Vimentin | Primary Antibodies | RV203 |
| MUB1901P-CE/IVD | Mouse anti-Vimentin | Primary Antibodies | RV203 |
| MUB1902P | Mouse anti-Vimentin | Primary Antibodies | V9 |
| MUB1902P-CE/IVD | Mouse anti-Vimentin | Primary Antibodies | V9 |
주문정보
| 제품번호 | 품명 | Clone | 제조사 | 비고 |
| MUB1900L2 | Mouse anti-Vimentin, conjugated with HRP | RV202 | Nordic-MUbio | Primary Antibody |
| MUB1903P | Recombinant mouse anti-Vimentin | RV204 | Nordic-MUbio | Monoclonal Antibody |
| MUB1904P | Recombinant rabbit anti-Vimentin | RV205 | Nordic-MUbio | Monoclonal Antibody |
| MUB1900L1 | Mouse anti-Vimentin, conjugated with FITC | RV202 | Nordic-MUbio | Primary Antibody |
| MUB1900L1-CE/IVD | Mouse anti-Vimentin, conjugated with FITC | RV202 | Nordic-MUbio | Primary Antibody |
| MUB1900P-CE/IVD | Mouse anti-Vimentin | RV202 | Nordic-MUbio | Monoclonal Antibody |
| MUB1900P | Mouse anti-Vimentin | RV202 | Nordic-MUbio | Primary Antibody |
| MUB1901P | Mouse anti-Vimentin | RV203 | Nordic-MUbio | Primary Antibody |
| MUB1901P-CE/IVD | Mouse anti-Vimentin | RV203 | Nordic-MUbio | Primary Antibody |
| MUB1902P | Mouse anti-Vimentin | V9 | Nordic-MUbio | Primary Antibody |
| MUB1902P-CE/IVD | Mouse anti-Vimentin | V9 | Nordic-MUbio | Primary Antibody |
관련정보
| 제품 자료 | Mouse anti-Vimentin-web.pdf | Upload date: 2022.02.21 |
| 제품 MSDS | ||
| 제품 메뉴얼 |