Project Description

HOME/Mouse anti M13 bacteriophage

Mouse anti M13 bacteriophage (g3p)

제품소개

M13 항체는 phage display library, 특히 single-chain antibody library 의 스크리닝에 많이 사용됩니다.
Mouse anti M13 Bacteriophage antibody는 M13 Bacteriophage가 숙주 세포로 유입되는데 중요한 역할을 하는 GP3(G3P) phage coat protein을 인식합니다.
그러므로 M13 시스템에서 개발된 ScFv 항체의 결합을 검출하는 데에도 활용될 수 있습니다.

Related brand: Nordic, Exalpha, Gallus, MUbio, Biologo, ADG, ELTI, Scicons, Bii

Source

Hybridoma is produced by the fusion of splenocytes from mice immunized with isolated M13 phage coat proteins and mouse myeloma cells.
Immunogen: M13 phage coat proteins.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

제품 사양

Clone E1
Isotype IgG3
Product Type Monoclonal Antibody
Units 100 µg
Host Mouse
Species Reactivity M13 phage
Application ELISA
Flow Cytometry
Immunohistochemistry
Western Blotting

Applications

Data represents absorbancy readings for A10B phage on rabbit IgG (A10B/IgG), A10B phage on BSA (A10B/BSA), streptavidin on rabbit IgG (SA/IgG), and streptavidin on BSA (SA/BSA) for each dilution of biotinylated anti-M13 monoclonal antibody.

References

1- Van Wezenbeek P.M., Schoenmakers J.G.; Nucleotide sequence of the genes III, VI and I of bacteriophage M13; Nucleic Acids Res. 6:2799-2818(1979).
2- Van Wezenbeek P.M., Hulsebos T.J., Schoenmakers J.G.; Nucleotide sequence of the filamentous bacteriophage M13 genome: comparison with phage fd; Gene 11:129-148(1980).
3- Cleary J.M., Ray D.S.; Deletion analysis of the cloned replication origin region from bacteriophage M13; J. Virol. 40:197-203(1981).
4- Hines J.C., Ray D.S.; Construction and characterization of new coliphage M13 cloning vectors; Gene 11:207-218(1980).
5- Yanisch-Perron C., Vieira J., Messing J.; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors; Gene 33:103-119(1985).
6- Messing J.; New M13 Vectors for Cloning; Meth. Enzymol. 101:20 78(1983).
7- Sanger F., Nicklen S., Coulson A.R.; DNA sequencing with chain-terminating inhibitors; Proc. Natl. Acad. Sci. U.S.A. 74:5463-5468(1977).
8- Zoller M.J., Smith M.; Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors; Meth. Enzymol. 100:468-500(1983).
9- Hu N.T., Messing J.; The making of strand-specific M13 probes; Gene 17:271-277(1982).
10- Heidecker G., Messing J., Gronenborn B.; A versatile primer for DNA sequencing in the M13mp2 cloning system; Gene 10:69-73(1980).
11- Ebright R., Dong Q., Messing J.; Corrected nucleotide sequence of M13mp18 gene III; Gene 114:81-83(1992).
12- Hong G.F.; A method for sequencing single-stranded cloned DNA in both directions; Biosci. Rep. 1:243-252(1981).
13- Messing J.; Multipurpose cloning system based on the single-stranded DNA bacteriophage M13; Recombinant DNA Technical Bulletin NIH 2:43-48(1979).

주문정보

제품번호 품명 Clone 제조사 비고
Z115S Mouse anti M13 bacteriophage (g3p) E1 Exalpha Biologicals
Z110M Mouse anti M13 bacteriophage (g3p), conjugated with Biotin E1 Exalpha Biologicals

관련정보

제품 자료 exalpha-Z115S.pdf
exalpha-Z110M.pdf
Upload date: 2022.03.11
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