Project Description
Synthelis Membrane Proteins
제품 소개
Synthelis 사는 막단백질 (ion channel, membrane transporter, GPCR, membrane enzyme and antigen)의 생산, 정제, characterization에 특화된 회사입니다.
screening & display technologies, antibody & vaccine development, structural biology, protein vectorization 등의 분야에 활용 가능한 순도 높은 단백질을 제공합니다.
특허 받은 cell-free technology 덕분에 다양한 종류와 포멧에서 적합한 단백질을 선택할 수 있습니다. 물론 필요에 따라 변형도 가능합니다.
Synthelis의 발현 시스템에서 새로 합성된 막 단백질은 생산 과정에서 평균 100nm liposome에 직접 삽입됩니다.
또한 detergent solution에 녹여 제공도 가능합니다. 단백질의 기능적 구조를 보존하면서 3D 결정을 형성하는 데에는 후자가 더 적합합니다.
제품 특징
Synthelis의 혁신적인 cell-free technology는 활성 단백질의 맞춤형 발현 및 characterization을 가능하게 하며, Proteoliposomes에 포장되거나 detergent solution 으로 제공됩니다.
이것은 모든 유형의 단백질에 적합하지만 특히 사용 가능한 형태로 생산하기 어려운 것으로 잘 알려진 막 단백질의 발현에 적합합니다.
이 기술은 세포 기반 시스템의 생리학적 제약으로부터 독립적이며 그로 인해 가용성 또는 독성 단백질을 포함하여 발현하기 어려운 모든 단백질의 발현에 효과적입니다.
다른 expression systems과 비교했을 때, Synthelis의 cell-free technology 가 같는 특징은 다음과 같습니다.
• 광범위한 단백질 변형의 Fast expression
• 기능이 완전히 보존된 전체 길이 단백질
• 다량의 단백질(10~100mg) 스케일업 가능
• 빠르고 경제적인 단백질 라벨링(구조적 및 기능적 목적)
• 단백질 종류에 따라 90% 이상의 성공률.
• 재발현 요청시 짧은 생산 시간: 단백질 mg당 1-2주
Application
• Vaccine development
• Antibody development
• Drug discovery
• Protein delivery
• In Vitro Diagnostics-IVD
• Structural biology
제품 사양
Families | Formats | Modified on demand |
Viral protein GPCR Ion Channel Ligand Porin Enzyme Transcriptional Regulator Transporter |
Proteoliposome Soluble detergent |
Fluorescent Isotopic labeling Incorporation of unnatural amino acids Truncated or Mutated forms |
주문정보
Viral protein
Acronym | Protein | Origin | Family | Format | Reference | Quantity (ug) | Learn more |
E1 | Envelop protein 1 | Hepatitis C virus |
Viral protein | Proteoliposome | PL011 | 10, 20, 50, 100, 200, 500 | See datasheet |
E2 | Envelop protein 2 | Hepatitis C virus |
Viral protein | Proteoliposome | PL010 | 10, 20, 50, 100, 200, 500 | See datasheet |
N-protein | Nucleocapsid Protein (COVID-19) | SARS-Cov 2 virus | Viral protein | Soluble | S-144 | 10, 20, 50, 100, 200, 500 | Learn more |
S1-RBD | ACE2 Receptor binding domain (COVID-19) | SARS-Cov 2 virus | Viral protein | Soluble | S-146 | 10, 20, 50, 100, 200, 500 | |
F5L | Vaccinia virus major membrane protein | Virus | Viral protein | Proteoliposome | PL007 | 10, 20, 50, 100, 200, 500 | See datasheet |
HCV p7 | Hepatitis C virus P7 | Virus | Viral protein | Proteoliposome | PL042 | 10, 20, 50, 100, 200, 500 | See datasheet |
GPCR
Acronym | Protein | Origin | Family | Format | Reference | Quantity (ug) | Learn more |
APJ/AR | Apelin Receptor | Human | GPCR | Proteoliposome | PL061 | 10, 20, 50, 100, 200, 500 | See datasheet |
B2R | Bradykinin receptor 2 | Human | GPCR | Proteoliposome | PL080 | 10, 20, 50, 100, 200, 500 | See datasheet |
BR | Bacterior-hodopsin | Bacteria | GPCR | Proteoliposome | PL027 | 10, 20, 50, 100, 200, 500 | See datasheet |
CCR1 | C-C chemokine receptor type 1 | Human | GPCR | Proteoliposome | PL021 | 10, 20, 50, 100, 200, 500 | See datasheet |
CCR2a | C-C chemokine receptor type 2, isoform a | Human | GPCR | Proteoliposome | PL022 | 10, 20, 50, 100, 200, 500 | See datasheet |
CCR2b | C-C chemokine receptor type 2, isoform b | Human | GPCR | Proteoliposome | PL023 | 10, 20, 50, 100, 200, 500 | See datasheet |
CCR5 | C-C chemokine receptor type 5 | Human | GPCR | Proteoliposome | PL024 | 10, 20, 50, 100, 200, 500 | See datasheet |
CXCR4 | C-X-C chemokine receptor type 4 | Human | GPCR | Proteoliposome | PL016 | 10, 20, 50, 100, 200, 500 | See datasheet |
CXCR4 | C-X-C chemokine receptor type 4 | Human | GPCR | Detergent | D016 | 10, 20, 50, 100, 200, 500 | See datasheet |
GLP1R | Glucagon-like peptide 1 receptor | Human | GPCR | Proteoliposome | PL067 | 10, 20, 50, 100, 200, 500 | See datasheet |
GPR50 | G-protein coupled receptor 50 | Human | GPCR | Proteoliposome | PL095 | 10, 20, 50, 100, 200, 500 | Learn more |
GPR54 | G-protein coupled receptor 54 | Human | GPCR | Proteoliposome | PL097 | 10, 20, 50, 100, 200, 500 | Learn more |
HMOP | Mu-type opioid receptor | Human | GPCR | Proteoliposome | PL025 | 10, 20, 50, 100, 200, 500 | See datasheet |
MT1 | Melatonin receptor | Human | GPCR | Proteoliposome | PL028 | 10, 20, 50, 100, 200, 500 | See datasheet |
RXFP1 | Relaxin receptor 1 | Human | GPCR | Proteoliposome | PL068 | 10, 20, 50, 100, 200, 500 | See datasheet |
Ion Channel
Acronym | Protein | Origin | Family | Format | Reference | Quantity (ug) | Learn more |
Kir1.1b | ATP-sensitive inward rectifier potassium channel 1 isoform b | Mouse | Ion channel | Proteoliposome | PL031 | 10, 20, 50, 100, 200, 500 | See datasheet |
kv1.2 | Potassium voltage-gated channel subfamily A, member 2 | Rat | Ion channel | Proteoliposome | PL037 | 10, 20, 50, 100, 200, 500 | See datasheet |
kv1.3 | Potassium voltage-gated channel subfamily A, member 3 | Mouse | Ion channel | Proteoliposome | PL036 | 10, 20, 50, 100, 200, 500 | See datasheet |
kv1.3 | Potassium voltage-gated channel subfamily A, member 3 | Mouse | Ion channel | Detergent | D036 | 10, 20, 50, 100, 200, 500 | See datasheet |
VDAC1 | Voltage-Dependent Anion Channel 1 | Human | Ion channel | Proteoliposome | PL029 | 10, 20, 50, 100, 200, 500 | See datasheet |
VDAC1 | Voltage-Dependent Anion Channel 1 | Human | Ion channel | Detergent | D029 | 10, 20, 50, 100, 200, 500 | See datasheet |
Ligand
Acronym | Protein | Origin | Family | Format | Reference | Quantity (ug) | Learn more |
SDF1a | Stromal cell-derived factor 1 | Human | Ligand | Soluble | S069 | 10, 20, 50, 100, 200, 500 | See datasheet |
Porin
Acronym | Protein | Origin | Family | Format | Reference | Quantity (ug) | Learn more |
OEP24 | Outer envelope pore protein 24, chloroplastic | Plant | Porin | Proteoliposome | PL040 | 10, 20, 50, 100, 200, 500 | See datasheet |
Enzyme
Acronym | Protein | Origin | Family | Format | Reference | Quantity (ug) | Learn more |
gp91phox (Nox2 subunit) |
gp91phox | Human | Enzyme | Proteoliposome | PL008 | 10, 20, 50, 100, 200, 500 | See datasheet |
KMO | Kynurenine 3-monooxygenase | Human | Enzyme | Proteoliposome | PL005 | 10, 20, 50, 100, 200, 500 | See datasheet |
Nox2, p22 Phox | Cytochrome b-245 light chain | Human | Enzyme | Proteoliposome | PL039 | 10, 20, 50, 100, 200, 500 | See datasheet |
Nox4 | Nox4 | Mouse | Enzyme | Proteoliposome | PL059 | 10, 20, 50, 100, 200, 500 | See datasheet |
PORCN | Protein-Cysteine N-palmitoyltransferase porcupine | Human | Enzyme | Proteoliposome | PL006 | 10, 20, 50, 100, 200, 500 | See datasheet |
TF | Tissue factor | Human | Enzyme | Proteoliposome | PL053 | 10, 20, 50, 100, 200, 500 | See datasheet |
Transcriptional Regulator
Acronym | Protein | Origin | Family | Format | Reference | Quantity (ug) | Learn more |
AROS | Active regulator of SIRT1 | Human | Transcriptional regulator | Proteoliposome | S052 | 10, 20, 50, 100, 200, 500 | See datasheet |
Transporter
Acronym | Protein | Origin | Family | Format | Reference | Quantity (ug) | Learn more |
Bak | Bcl-2 homologous antagonist killer | Human | Transporter | Proteoliposome | PL038 | 110, 20, 50, 100, 200, 500 | See datasheet |
EspH | Cytoskeleton-modulating effector of enterohaemorrhagic and enteropathogenic Escherichia coli | Bacteria | Transporter | Proteoliposome | PL051 | 10, 20, 50, 100, 200, 500 | See datasheet |
HCV p7 | Hepatitis C virus P7 | Virus | Transporter | Proteoliposome | PL042 | 10, 20, 50, 100, 200, 500 | See datasheet |
NhaA | Na(+)/H(+) antiporter NhaA | E. coli | Transporter | Proteoliposome | PL032 | 10, 20, 50, 100, 200, 500 | See datasheet |
UCP1 | Mitochondrial brown fat uncoupling protein 1 | Mitochondria | Transporter | Proteoliposome | PL047 | 10, 20, 50, 100, 200, 500 | See datasheet |
* other quantities can be delivered upon request
Case Studies
Application Notes
Label-free Kinetic Analysis of Membrane Protein Interactions Using Biolayer Interferometry (BLI)
Validation of the activity of GPCRs using SPRI: case study using functional CxCR4
Synthelis Publications
Cholesterol impacts chemokine CCR5 receptor ligand-binding activity. Pierre Calmet, Christophe Cullin, Sandra Cortès, Maylou Vang, Nada Caudy, Rim Baccouch, Jean Dessolin, Nada Taib Maamar, Sophie Lecomte, Bruno Tillier, Isabel D Alves in FEBS J. 2020
High throughput cell-free expression: From PCR product design to functionality validation. Sandra Cortès, Fatima-Ezzahra Hibti, Frydman Chiraz, Safia Ezzine in Methods and Protocols. 2019
Functional characterization of cell-free expressed Kv1.3 channel using a voltage-sensitive fluorescent dye. Sandra Cortès, Caroline Barette, Rémy Béroud, Michel De Waard, Béatrice Schaack in Elsevier. 2018
A new functional membrane protein microarray based on tethered phospholipid bilayers. Meriem Chadli, Ofelia Maniti, Christophe Marquette, Bruno Tillier, Sandra Cortès and Agnès Girard-Egrot in the Analyst. 2018
New Tethered Phospholipid Bilayers Integrating Functional G-Protein-Coupled Receptor Membrane Proteins. Meriem Chadli, Samuel Rebaud, Ofelia Maniti, Bruno Tillier, Sandra Cortès, and Agnes Girard-Egrot in Langmuir. 2017
Coupling neutron reflectivity with cell-free protein synthesis to probe membrane protein structure in supported bilayers. Thomas Soranzo, Donald K. Martin, Jean-Luc Lenormand, and Erik B. Watkins in Scientific Reports. 2017
Functional reconstitution of cell-free synthesized purified Kv channels. Stéphane Renauld , Sandra Cortès , Beate Bersch, Xavier Henry, Michel De Waard , Béatrice Schaack in Biochim Biophys Acta. 2017
Therapeutic effects of proteoliposomes on X-linked chronic granulomatous disease: proof of concept using macrophages differentiated from patient-specific induced pluripotent stem cells Julie Brault, Guillaume Vaganay, Aline Le Roy, Jean-Luc Lenormand, Sandra Cortès, Marie José Stasia in International Journal of Nanomedicine. 2017
Anti-Tumor Effects of Bak-Proteoliposomes against Glioblastoma Lavinia, Liguori, Fabio Pastorino , Xavier Rousset, Silvia Alfano, Sandra Cortès, Laura Emionite, Antonio Daga, Mirco Ponzoni and Jean-Luc Lenormand in Molecules. 2015
Functional characterization of p7 viroporin from hepatitis C virus produced in a cell-free expression system. Soranzo T, Cortès S, Gilde F, Kreir M, Picart C, Lenormand JL in Protein Expr Purif. 2015
Recombinant Nox4 cytosolic domain produced by a cell or cell-free base systems exhibits constitutive diaphorase activity.
Nguyen MV, Zhang L, Lhomme S, Mouz N, Lenormand JL, Lardy B, Morel F in Biochem Biophys Res Commun. 2012 16;419(3):453-8.
Crystallization of the membrane protein hVDAC1 produced in cell-free system. Deniaud A, Liguori L, Blesneac I, Lenormand JL, Pebay-Peyroula E in
Biochim Biophys Acta. 2010 -1798(8):1540-6.
Single-step production of functional OEP24 proteoliposomes. Liguori L, Blesneac I, Madern D, Vivaudou M, Lenormand JL in Protein Expr Purif. 2010 – 69,1 (106-111).
Production of recombinant proteoliposomes for therapeutic uses. Liguori L. and Lenormand JL in Methods in Enzymology, 2009 – 465 (209-223).
A bacterial cell-free expression system to produce membrane proteins and proteoliposomes: from cDNA to functional assay. Liguori L, Marques B, Lenormand JL in Curr Protoc Protein Sci. 2008. Nov;Chapter 5:Unit 5.22.
Liposomes-mediated delivery of pro-apoptotic therapeutic membrane proteins. Liguori L, Marques B, Villegas-Mendez A, Rothe R, Lenormand JL in J Control Release. 2008. 20;126(3):217-27.
Liposome-mediated cellular delivery of active gp91(phox). Marques B, Liguori L, Paclet MH, Villegas-Mendéz A, Rothe R, Morel F, Lenormand JL in PLoS One. 2007. 12;2(9):e856. PDF here.
Production of membrane proteins using cell-free expression systems. Liguori L, Marques B, Villegas-Méndez A, Rothe R, Lenormand JL in Expert Rev Proteomics. 2007. 4(1):79-90. Review.
Cell-free Publications
Liposome chaperon in cell-free membrane protein synthesis: one-step preparation of KcsA-integrated liposomes and electrophysiological analysis by the planar bilayer method. Ando M, Akiyama M, Okuno D, Hirano M, Ide T, Sawada S, Sasaki T, Akiyoshi K in Biomater Sci 2015
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