Project Description

Membrane Proteins

제품 소개

Synthelis 사는 막단백질 (ion channel, membrane transporter, GPCR, membrane enzyme and antigen)의 생산, 정제, characterization에 특화된 회사입니다.
screening & display technologies, antibody & vaccine development, structural biology, protein vectorization 등의 분야에 활용 가능한 순도 높은 단백질을 제공합니다.
특허 받은 cell-free technology 덕분에 다양한 종류와 포멧에서 적합한 단백질을 선택할 수 있습니다. 물론 필요에 따라 변형도 가능합니다.

Synthelis의 발현 시스템에서 새로 합성된 막 단백질은 생산 과정에서 평균 100nm liposome에 직접 삽입됩니다.
또한 detergent solution에 녹여 제공도 가능합니다. 단백질의 기능적 구조를 보존하면서 3D 결정을 형성하는 데에는 후자가 더 적합합니다.

제품 특징

Synthelis의 혁신적인 cell-free technology는 활성 단백질의 맞춤형 발현 및 characterization을 가능하게 하며, Proteoliposomes에 포장되거나 detergent solution 으로 제공됩니다.
이것은 모든 유형의 단백질에 적합하지만 특히 사용 가능한 형태로 생산하기 어려운 것으로 잘 알려진 막 단백질의 발현에 적합합니다.
이 기술은 세포 기반 시스템의 생리학적 제약으로부터 독립적이며 그로 인해 가용성 또는 독성 단백질을 포함하여 발현하기 어려운 모든 단백질의 발현에 효과적입니다.

다른 expression systems과 비교했을 때, Synthelis의 cell-free technology 가 같는 특징은 다음과 같습니다.

• 광범위한 단백질 변형의 Fast expression
• 기능이 완전히 보존된 전체 길이 단백질
• 다량의 단백질(10~100mg) 스케일업 가능
• 빠르고 경제적인 단백질 라벨링(구조적 및 기능적 목적)
• 단백질 종류에 따라 90% 이상의 성공률.
• 재발현 요청시 짧은 생산 시간: 단백질 mg당 1-2주

Application

• Vaccine development
• Antibody development
• Drug discovery
• Protein delivery
• In Vitro Diagnostics-IVD
• Structural biology

제품 사양

Families Formats Modified on demand
Viral protein
GPCR
Ion Channel
Ligand
Porin
Enzyme
Transcriptional Regulator
Transporter
Proteoliposome
Soluble detergent
Fluorescent
Isotopic labeling
Incorporation of unnatural amino acids
Truncated or Mutated forms

주문정보

Viral protein

Acronym  Protein  Origin Family  Format Reference Quantity (ug)  Learn more
E1  Envelop protein 1  Hepatitis C
virus
Viral protein  Proteoliposome PL011 10, 20, 50, 100, 200, 500  See datasheet
E2  Envelop protein 2  Hepatitis C
virus
Viral protein  Proteoliposome PL010 10, 20, 50, 100, 200, 500  See datasheet
N-protein Nucleocapsid Protein (COVID-19) SARS-Cov 2 virus Viral protein Soluble S-144 10, 20, 50, 100, 200, 500 Learn more
S1-RBD ACE2 Receptor binding domain (COVID-19) SARS-Cov 2 virus Viral protein Soluble S-146 10, 20, 50, 100, 200, 500
F5L  Vaccinia virus major membrane protein  Virus Viral protein  Proteoliposome PL007 10, 20, 50, 100, 200, 500  See datasheet
HCV p7  Hepatitis C virus P7  Virus Viral protein  Proteoliposome PL042 10, 20, 50, 100, 200, 500  See datasheet


GPCR

Acronym Protein Origin Family Format Reference Quantity (ug) Learn more
APJ/AR Apelin Receptor Human GPCR Proteoliposome PL061 10, 20, 50, 100, 200, 500  See datasheet
B2R Bradykinin receptor 2 Human GPCR Proteoliposome PL080 10, 20, 50, 100, 200, 500  See  datasheet
BR Bacterior-hodopsin Bacteria GPCR Proteoliposome PL027 10, 20, 50, 100, 200, 500  See datasheet
CCR1 C-C chemokine receptor type 1 Human GPCR Proteoliposome PL021 10, 20, 50, 100, 200, 500  See datasheet
CCR2a C-C chemokine receptor type 2, isoform a Human GPCR Proteoliposome PL022 10, 20, 50, 100, 200, 500  See datasheet
CCR2b C-C chemokine receptor type 2, isoform b Human GPCR Proteoliposome PL023 10, 20, 50, 100, 200, 500  See datasheet
CCR5 C-C chemokine receptor type 5 Human GPCR Proteoliposome PL024 10, 20, 50, 100, 200, 500  See datasheet
CXCR4 C-X-C chemokine receptor type 4 Human GPCR Proteoliposome PL016 10, 20, 50, 100, 200, 500  See datasheet
CXCR4 C-X-C chemokine receptor type 4 Human GPCR Detergent D016 10, 20, 50, 100, 200, 500  See datasheet
GLP1R Glucagon-like peptide 1 receptor Human GPCR Proteoliposome PL067 10, 20, 50, 100, 200, 500  See datasheet
GPR50 G-protein coupled receptor 50 Human GPCR Proteoliposome PL095 10, 20, 50, 100, 200, 500 Learn more
GPR54 G-protein coupled receptor 54 Human GPCR Proteoliposome PL097 10, 20, 50, 100, 200, 500 Learn more
HMOP Mu-type opioid receptor Human GPCR Proteoliposome PL025 10, 20, 50, 100, 200, 500  See datasheet
MT1 Melatonin receptor Human GPCR Proteoliposome PL028 10, 20, 50, 100, 200, 500  See datasheet
RXFP1 Relaxin receptor 1 Human GPCR Proteoliposome PL068 10, 20, 50, 100, 200, 500  See datasheet


Ion Channel

Acronym Protein Origin Family Format Reference Quantity (ug) Learn more
Kir1.1b ATP-sensitive inward rectifier potassium channel 1 isoform b Mouse Ion channel Proteoliposome PL031 10, 20, 50, 100, 200, 500  See datasheet
kv1.2 Potassium voltage-gated channel subfamily A, member 2 Rat Ion channel Proteoliposome PL037 10, 20, 50, 100, 200, 500  See datasheet
kv1.3 Potassium voltage-gated channel subfamily A, member 3 Mouse Ion channel Proteoliposome PL036 10, 20, 50, 100, 200, 500  See datasheet
kv1.3 Potassium voltage-gated channel subfamily A, member 3 Mouse Ion channel Detergent D036 10, 20, 50, 100, 200, 500  See datasheet
VDAC1 Voltage-Dependent Anion Channel 1 Human Ion channel Proteoliposome PL029 10, 20, 50, 100, 200, 500  See datasheet
VDAC1 Voltage-Dependent Anion Channel 1 Human Ion channel Detergent D029 10, 20, 50, 100, 200, 500  See datasheet


Ligand

Acronym Protein Origin Family Format Reference Quantity (ug) Learn more
SDF1a Stromal cell-derived factor 1 Human Ligand Soluble S069 10, 20, 50, 100, 200, 500  See datasheet


Porin

Acronym Protein Origin Family Format Reference Quantity (ug) Learn more
OEP24 Outer envelope pore protein 24, chloroplastic Plant Porin Proteoliposome PL040 10, 20, 50, 100, 200, 500  See datasheet


Enzyme

Acronym Protein Origin Family Format Reference Quantity (ug) Learn more
gp91phox
(Nox2 subunit)
gp91phox Human Enzyme Proteoliposome PL008 10, 20, 50, 100, 200, 500  See datasheet
KMO Kynurenine 3-monooxygenase Human Enzyme Proteoliposome PL005 10, 20, 50, 100, 200, 500  See datasheet
Nox2, p22 Phox Cytochrome b-245 light chain Human Enzyme Proteoliposome PL039 10, 20, 50, 100, 200, 500  See datasheet
Nox4 Nox4 Mouse Enzyme Proteoliposome PL059 10, 20, 50, 100, 200, 500  See datasheet
PORCN Protein-Cysteine    N-palmitoyltransferase porcupine Human Enzyme Proteoliposome PL006 10, 20, 50, 100, 200, 500 See datasheet
TF Tissue factor Human Enzyme Proteoliposome PL053 10, 20, 50, 100, 200, 500  See datasheet


Transcriptional Regulator

Acronym Protein Origin Family Format Reference Quantity (ug) Learn more
AROS Active regulator of SIRT1 Human Transcriptional regulator Proteoliposome S052 10, 20, 50, 100, 200, 500  See datasheet


Transporter

Acronym Protein Origin Family Format Reference Quantity (ug) Learn more
Bak Bcl-2 homologous antagonist killer Human Transporter Proteoliposome PL038 110, 20, 50, 100, 200, 500  See datasheet
EspH Cytoskeleton-modulating effector of enterohaemorrhagic and enteropathogenic Escherichia coli Bacteria Transporter Proteoliposome PL051 10, 20, 50, 100, 200, 500  See datasheet 
HCV p7 Hepatitis C virus P7 Virus Transporter Proteoliposome PL042 10, 20, 50, 100, 200, 500  See datasheet
NhaA Na(+)/H(+) antiporter NhaA E. coli Transporter Proteoliposome PL032 10, 20, 50, 100, 200, 500  See datasheet
UCP1 Mitochondrial brown fat uncoupling protein 1 Mitochondria Transporter Proteoliposome PL047 10, 20, 50, 100, 200, 500  See datasheet

* other quantities can be delivered upon request

Case Studies

Solubilisation of a strongly aggregated vaccine antigen (research project performed for Sanofi Pasteur)


Application Notes

Validation of a new method to characterize proteoliposome in solution by force spectroscopy with atomic force microscopy

Label-free Kinetic Analysis of Membrane Protein Interactions Using Biolayer Interferometry (BLI)

Successful production of antibodies against functional GPCR : Case study using functional CXCR4-containing liposomes

Validation of the activity of GPCRs using SPRI: case study using functional CxCR4


Synthelis Publications

Cholesterol impacts chemokine CCR5 receptor ligand-binding activity. Pierre Calmet, Christophe Cullin, Sandra Cortès, Maylou Vang, Nada Caudy, Rim Baccouch, Jean Dessolin, Nada Taib Maamar, Sophie Lecomte, Bruno Tillier, Isabel D Alves in FEBS J. 2020

High throughput cell-free expression: From PCR product design to functionality validation. Sandra Cortès, Fatima-Ezzahra Hibti, Frydman Chiraz, Safia Ezzine in Methods and Protocols. 2019

Functional characterization of cell-free expressed Kv1.3 channel using a voltage-sensitive fluorescent dye. Sandra Cortès, Caroline Barette, Rémy Béroud, Michel De Waard, Béatrice Schaack in Elsevier. 2018

A new functional membrane protein microarray based on tethered phospholipid bilayers. Meriem Chadli, Ofelia Maniti, Christophe Marquette, Bruno Tillier, Sandra Cortès and Agnès Girard-Egrot in the Analyst. 2018

New Tethered Phospholipid Bilayers Integrating Functional G-Protein-Coupled Receptor Membrane Proteins. Meriem Chadli, Samuel Rebaud, Ofelia Maniti, Bruno Tillier, Sandra Cortès, and Agnes Girard-Egrot in Langmuir2017

Coupling neutron reflectivity with cell-free protein synthesis to probe membrane protein structure in supported bilayers. Thomas Soranzo, Donald K. Martin, Jean-Luc Lenormand, and Erik B. Watkins in Scientific Reports2017

Functional reconstitution of cell-free synthesized purified Kv channels. Stéphane Renauld , Sandra Cortès , Beate Bersch, Xavier Henry, Michel De  Waard , Béatrice Schaack in Biochim Biophys Acta. 2017

Therapeutic effects of proteoliposomes on X-linked chronic granulomatous disease: proof of concept using macrophages differentiated from patient-specific induced pluripotent stem cells Julie Brault, Guillaume Vaganay, Aline Le Roy, Jean-Luc Lenormand, Sandra Cortès, Marie José Stasia in International Journal of Nanomedicine2017

Anti-Tumor Effects of Bak-Proteoliposomes against Glioblastoma Lavinia, Liguori, Fabio Pastorino , Xavier Rousset, Silvia Alfano, Sandra Cortès, Laura Emionite, Antonio Daga, Mirco Ponzoni and Jean-Luc Lenormand in Molecules2015

Functional characterization of p7 viroporin from hepatitis C virus produced in a cell-free expression system. Soranzo T, Cortès S, Gilde F, Kreir M, Picart C, Lenormand JL in Protein Expr Purif2015

Recombinant Nox4 cytosolic domain produced by a cell or cell-free base systems exhibits constitutive diaphorase activity.
Nguyen MV, Zhang L, Lhomme S, Mouz N, Lenormand JL, Lardy B, Morel F in Biochem Biophys Res Commun2012  16;419(3):453-8.

Crystallization of the membrane protein hVDAC1 produced in cell-free system. Deniaud A, Liguori L, Blesneac I, Lenormand JL, Pebay-Peyroula E in
Biochim Biophys Acta. 2010 -1798(8):1540-6.

Single-step production of functional OEP24 proteoliposomes. Liguori L, Blesneac I, Madern D, Vivaudou M, Lenormand JL in  Protein Expr Purif2010 – 69,1 (106-111).

Production of recombinant proteoliposomes for therapeutic uses. Liguori L. and Lenormand JL in  Methods in Enzymology2009 – 465 (209-223).

A bacterial cell-free expression system to produce membrane proteins and proteoliposomes: from cDNA to functional assay. Liguori L, Marques B, Lenormand JL in Curr Protoc Protein Sci2008. Nov;Chapter 5:Unit 5.22.

Liposomes-mediated delivery of pro-apoptotic therapeutic membrane proteins. Liguori L, Marques B, Villegas-Mendez A, Rothe R, Lenormand JL in J Control Release2008. 20;126(3):217-27.

Liposome-mediated cellular delivery of active gp91(phox). Marques B, Liguori L, Paclet MH, Villegas-Mendéz A, Rothe R, Morel F, Lenormand JL in PLoS One2007. 12;2(9):e856. PDF here.

Production of membrane proteins using cell-free expression systems. Liguori L, Marques B, Villegas-Méndez A, Rothe R, Lenormand JL in Expert Rev Proteomics2007. 4(1):79-90. Review.


Cell-free Publications

Liposome chaperon in cell-free membrane protein synthesis: one-step preparation of KcsA-integrated liposomes and electrophysiological analysis by the planar bilayer method. Ando M, Akiyama M, Okuno D, Hirano M, Ide T, Sawada S, Sasaki T, Akiyoshi K in Biomater Sci 2015

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