Magnetic Beads

제품 소개

GenScript는 항체와 epitope-tagged proteins의 빠르고 효율적인 purification이 가능하도록 super paramagnetic MagBeads를 제공합니다.
GenScript MagBeads는 항체와 epitope-tagged proteins에 대한 매우 특이적인 친화성을 가지게 하는 recombinant protein ligands로 코팅되어 있습니다.
magnetic protein purification method 는 세포분해효소로부터 직접 단백질 purification이 가능하기 때문에 원심분리 단계가 필요 없습니다.
GenScript MagBeads는 높은 binding capacity, binding specificity 및 즉각적인 반응성을 보이므로 많은 샘플을 빠른 시간에 처리하길 원하는 application에 매우 이상적인 제품입니다.

제품 특징

• 빠르고 쉽고 편리한 자성 분리
• 높은 바인딩 능력
• 일관된 품질의 비드 사용으로 인한 재현성 있는 결과
• protein degradation 최소화

Applications

• Antibody purification
• Purification of tagged proteins e.g. 6x His, GST, biotin etc.
• High throughput protein and antibody purification

Selection guide

Application	Product	Technology	Binding capacity	Size	Cat.No.
Purification of antibodies from small and large scale cultures	Protein A MagBeads	Antibody capture through recombinant protein A or protein G	>10 mg rabbit IgG/ml settled beads	4 ml	L00273
	Protein A MagBeads MX		>30 mg human IgG /ml settled beads	4 ml	L00672
	Protein G MagBeads		> 10 mg goat IgG/ml settled beads	2 ml	L00274
	Protein G MagBeads MX		> 25 mg Human IgG per 1 ml settled beads	4 ml	L00673
	Protein A/G MagBeads		> 10 mg goat IgG/ml settled beads	2 ml	L00277
Alkaline stable antibody purification	AmMag™ Protein A Magnetic Beads	Antibody capture through alkaline- tolerant recombinant protein A	> 40 mg human IgG per 1 ml settled beads	4 ml	L00695
Purification of His-tagged proteins	Ni-charged MagBeads	His-tagged protein capture through pre-charged nickel ion	5 – 20 mg 6×His-tagged protein (27 kD)/ml settled beads	8 ml	L00295
	AmMag™ Ni Magnetic Beads	His-tagged protein capture through nickel-charged TED ligand. EDTA and DTT resistant. In absence of chelators, reusable over 100 times	> 10 mg 6x His-tagged protein/ml settled beads	4 ml	L00776-4
				40 ml	L00776-40
				400 ml	L00776-400
Purification of GST-tagged proteins	Glutathione MagBeads	GST fusion protein capture through reduced glutathione	20 – 30 mg GST/ml settled beads	8 ml	L00327
Purification of biotinylated molecules	Streptavidin MagBeads	Biotinylated molecule capture through pre-coupled streptavidin	> 60 nmol free biotin /ml settled beads	2 ml	L00424
^* Each size includes 75% Storage Buffer.

주문정보

Protein A, G and A/G MagBeads

제품번호	품명	규격	제조사	비고
L00273	Protein A MagBeads	4 ml	GenScript
L00672-4	Protein A MagBeads MX	4 ml	GenScript
L00274	Protein G MagBeads	2 ml	GenScript
L00673-4	Protein G MagBeads MX	4 ml	GenScript
L00277	Protein A/G MagBeads	2 ml	GenScript
L00695-4	AmMag™ Protein A Magnetic Beads	4 ml	GenScript

Tagged Protein Purification MagBeads

제품번호	품명	규격	제조사	비고
L00295	Ni-charged MagBeads	8 ml	GenScript
L00776-400	AmMag™ Ni Magnetic beads	400 ml	GenScript
L00776-40	AmMag™ Ni Magnetic beads	40 ml	GenScript
L00776-4	AmMag™ Ni Magnetic beads	4 ml	GenScript
L00327	Glutathione MagBeads	8 ml	GenScript
L00424	Streptavidin MagBeads	2 ml	GenScript
L00835-5	MonoRab™ Anti-DYKDDDDK Magnetic Beads	5 ml	GenScript
L00835-25	MonoRab™ Anti-DYKDDDDK Magnetic Beads	25 ml	GenScript
L00835-1	MonoRab™ Anti-DYKDDDDK Magnetic Beads	1 ml	GenScript

Customer Citations

Y Kuroda., et al. Improved capillary electrophoresis method for the analysis of carbohydrate-deficient transferrin in human serum, avoiding interference by complement C3. J Pharm Biomed Anal. 2013 Mar 25;76:81-6.

Hsieh YH., et al. Histone deacetylase inhibitor suberoylanilide hydroxamic acid suppresses the pro-oncogenic effects induced by hepatitis B virus pre-S2 mutant oncoprotein and represents a potential chemopreventive agent in high-risk chronic HBV patients. Carcinogenesis.. 2013 Feb;34(2):475-85.

Ferreira E., et al. Protein Interaction Studies Point To New Functions For Escherichia Coli Glyceraldehyde-3-Phosphate Dehydrogenase.Res Microbiol. 2013 Feb-Mar;164(2):145-54.

Tyler RE., et al. Unassembled CD147 is an endogenous endoplasmic reticulum-associated degradation substrate. Mol Biol Cell. 2012 Dec;23(24):4668-78.

Wiltrout E., et al. Misacylation Of Trna With Methionine In Saccharomyces Cerevisiae. Nucleic Acids Res. 2012 Nov 1;40(20):10494-506.

Priyanka Sivadas, et al. A Flagellar A-Kinase Anchoring Protein With Two Amphipathic Helices Forms A Structural Scaffold In The Radial Spoke Complex. J Cell Biol. 2012 Nov 12;199(4):639-51.

Dai C., et al.Loss Of Tumor Suppressor Nf1 Activates Hsf1 To Promote Carcinogenesis. J Clin Invest. 2012Oct 1;122(10):3742-54.

Leyme A., et al. Identification Of Ilk As A New Partner Of The Adam12 Disintegrin And Metalloprotease In Cell Adhesion And Survival. Mol Biol Cell. 2012 Sep;23(17):3461-72.

Dai MS., et al. Physical and functional interaction between ribosomal protein L11 and the tumor suppressor ARF.
J Biol Chem. 2012 May 18;287(21):17120-9.

Tang X., et al. A High-Throughput Screening Method for Small-Molecule Inhibitors of the Aberrant Mutant SOD1 and Dynein Complex Interaction. J Biomol Screen. 2012 Mar;17(3):314-26.

An J., et al. CHCM1/CHCHD6, Novel Mitochondrial Protein Linked to Regulation of Mitofilin and Mitochondrial Cristae Morphology. J Biol Chem. 2012 Mar 2;287(10):7411-26.

Kazuhiro Ishiguro, et al. Cutting Edge: Tubulin α Functions as an Adaptor in NFAT-importin β Interaction. J Immunol. 2011Mar 1;186(5):2710-3.

Luo MH., et al. Human Cytomegalovirus Infection Causes Premature and Abnormal Differentiation of Human Neural Progenitor Cells. J Virol. 2010 Apr;84(7):3528-41.

Dongzhu Ma, et al.Rheb GTPase Controls Apoptosis by Regulating Interaction of FKBP38 with Bcl-2 and Bcl-XL. J Biol Chem. 2010 Mar 19;285(12):8621-7.

Crystal Morales, et al. Drosophila Glycoprotein 93 Is an Ortholog of Mammalian Heat Shock Protein gp96 (grp94, HSP90b1, HSPC4) and Retains Disulfide Bond-Independent Chaperone Function for TLRs and Integrins. J Immunol. 2009 Oct 15;183(8):5121-8.

Cross BC., et al. Dissecting the physiological role of selective transmembrane-segment retention at the ER translocon.
J Cell Sci. 2009 Jun 1;122(Pt 11):1768-77.

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Project Description