Fig 1. Reliable performance after lyophilization in a multiplex setup
Multiplex amplification of SARS-CoV-2 N gene, E gene, and RdRp gene according to CDC recommended sequences (N2) and WHO/ Charité sequences (E and RdRp-P2) using Lyo-Ready Probe 1-Step Kit as a wet mix (purple bars) or reconstituted mix after lyophilization (blue bars). Ct values are shown in the top panels and efficiency in the bottom panels. 5 serial dilutions of RNA template were used, corresponding to 40000, 4000, 400, 40, and 4 copies of the SARS-CoV-2 genome. The total reaction volume was 20 μL. Cycle conditions were 55 °C 10 min, 95 °C 3 min and 50 cycles of 95 °C 15 s, 58 °C 30 s. LyoReady Probe 1-Step Kit shows no loss of efficiency or sensitivity after lyophilization.