Project Description

HOME/Consumable/Real-time PCR Master Mix/Lyo-Ready Probe 1-Step Kit

Lyo-Ready Probe 1-Step Kit

제품 소개

Lyo-Ready Probe 1-Step Kit는 바이러스 RNA의 초고감도 검출을 위해 설계된 동결건조 가능한 RT-qPCR 키트입니다.
hot start Taq polymerase, dNTPs, MgCl2 및 첨가제 혼합물을 함께 동결건조 한 후에도 활성 손실 없이 최적의 기능을 제공합니다.
특별히 제조된 이 키트는 diagnostic assays 개발에 이상적입니다.

제품특징

• RNA 바이러스의 초고감도 검출
• 표준 시약와 동일한 고성능
• glycerol-free 4x qPCR mix
• 별도의 고농도 UltraScript RTase 제공(RNase inhibitor 포함)
• SARS-CoV-2 핵산의 정성적 검출 검증
• 높은 처리량으로 multiplexed assays에 활용
• Pre-Lyo Probe 1-Step Evaluation Kit 별도 판매
• ISO 13485 certified Quality Management System

응용분야

• 동결건조 분자진단검사 개발
• 극도로 낮은 copy number 타겟 감지
• TaqMan®, Scorpions® and molecular beacon probes와 호환

보관온도

•-20℃

Fig 1. Reliable performance after lyophilization in a multiplex setup
Multiplex amplification of SARS-CoV-2 N gene, E gene, and RdRp gene according to CDC recommended sequences (N2) and WHO/ Charité sequences (E and RdRp-P2) using Lyo-Ready Probe 1-Step Kit as a wet mix (purple bars) or reconstituted mix after lyophilization (blue bars). Ct values are shown in the top panels and efficiency in the bottom panels. 5 serial dilutions of RNA template were used, corresponding to 40000, 4000, 400, 40, and 4 copies of the SARS-CoV-2 genome. The total reaction volume was 20 μL. Cycle conditions were 55 °C 10 min, 95 °C 3 min and 50 cycles of 95 °C 15 s, 58 °C 30 s. LyoReady Probe 1-Step Kit shows no loss of efficiency or sensitivity after lyophilization.

Fig 2. Reliable performance after lyophilization in a singleplex setup
Amplification of SARS-CoV-2 targets using Lyo-Ready Probe 1-Step Kit as a wet mix (purple bars) or reconstituted mix after lyophilization (blue bars) in singleplex. SARS-CoV-2 targets used were the N gene (CDC N1 and N2 sequences), E gene ( WHO/CDC sequences), and RdRp gene ( WHO sequences). Ct values are shown in the top panels and efficiency in the bottom panels. 5 serial dilutions of RNA template were used corresponding to 40000, 4000, 400, 40, and 4 copies of the SARS-CoV-2 genome. The total reaction volume was 20 μL. Cycle conditions were 55 °C 5 min, 95 °C 3 min and 50 cycles of 95°C 15 s, 58 °C 30 s. Lyo-Ready Probe 1-Step Kit shows no loss of efficiency or sensitivity after lyophilization.

주문정보

Cat. No. 품명 규격 제조사 비고
PB90.01-03 Lyo-Ready Probe Mix 600 x 20 μL Reactions PCRbiosystems

4x Lyo-Ready Probe Mix (3 x 1 mL)

PB90.01-10 Lyo-Ready Probe Mix 2,000 x 20 μL Reactions
(2 x 5 mL)
PCRbiosystems

4x Lyo-Ready Probe Mix (2 x 5 mL)

PB90.01-50 Lyo-Ready Probe Mix 10,000 x 20 μL Reactions
(1 x 50 mL)
PCRbiosystems  4x Lyo-Ready Probe Mix (1 x 50 mL)
PB90.12-10 Lyo-Ready Probe 1-Step Kit 2,000 x 20 μL Reactions
(2 x 5 mL)
PCRbiosystems 4x Lyo-Ready Probe Mix (2 x 5 mL)
3,200x UltraScript RTase (1 x 12.5 μL)
PB90.12-50 Lyo-Ready Probe 1-Step Kit 10,000 x 20 μL Reactions
(1 x 50 mL)
PCRbiosystems 4x Lyo-Ready Probe Mix (1 x 50 mL)
3,200x UltraScript RTase (1 x 62.5 μL)
PB90.11-03 Pre-Lyo Probe 1-Step Evaluation Kit 600 x 20 μL Reactions PCRbiosystems 4x Lyo-Ready Probe Mix (3 x 1 mL)
20x UltraScript RTase (1 x 600μL)

관련 논문

.

Document

제품 자료 Lyo-Ready-Probe-1-Step-Kit.pdf Upload date: 2021.12.27
제품 매뉴얼 PB90.01-Lyo-Ready-Probe-Mix.pdf
PB90.12-Lyo-Ready-Probe-1-Step-Kit.pdf
PB90.11-Pre-Lyo-Probe-1-Step-Evaluation-Kit.pdf
Upload date: 2021.12.27
제품 MSDS Upload date: 2021.12.27