Project Description
Lyo-Ready Probe 1-Step Kit
제품 소개
Lyo-Ready Probe 1-Step Kit는 바이러스 RNA의 초고감도 검출을 위해 설계된 동결건조 가능한 RT-qPCR 키트입니다.
hot start Taq polymerase, dNTPs, MgCl2 및 첨가제 혼합물을 함께 동결건조 한 후에도 활성 손실 없이 최적의 기능을 제공합니다.
특별히 제조된 이 키트는 diagnostic assays 개발에 이상적입니다.
제품특징
• RNA 바이러스의 초고감도 검출
• 표준 시약와 동일한 고성능
• glycerol-free 4x qPCR mix
• 별도의 고농도 UltraScript RTase 제공(RNase inhibitor 포함)
• SARS-CoV-2 핵산의 정성적 검출 검증
• 높은 처리량으로 multiplexed assays에 활용
• Pre-Lyo Probe 1-Step Evaluation Kit 별도 판매
• ISO 13485 certified Quality Management System
응용분야
• 동결건조 분자진단검사 개발
• 극도로 낮은 copy number 타겟 감지
• TaqMan®, Scorpions® and molecular beacon probes와 호환
보관온도
•-20℃
Fig 1. Reliable performance after lyophilization in a multiplex setup
Multiplex amplification of SARS-CoV-2 N gene, E gene, and RdRp gene according to CDC recommended sequences (N2) and WHO/ Charité sequences (E and RdRp-P2) using Lyo-Ready Probe 1-Step Kit as a wet mix (purple bars) or reconstituted mix after lyophilization (blue bars). Ct values are shown in the top panels and efficiency in the bottom panels. 5 serial dilutions of RNA template were used, corresponding to 40000, 4000, 400, 40, and 4 copies of the SARS-CoV-2 genome. The total reaction volume was 20 μL. Cycle conditions were 55 °C 10 min, 95 °C 3 min and 50 cycles of 95 °C 15 s, 58 °C 30 s. LyoReady Probe 1-Step Kit shows no loss of efficiency or sensitivity after lyophilization.
Fig 2. Reliable performance after lyophilization in a singleplex setup
Amplification of SARS-CoV-2 targets using Lyo-Ready Probe 1-Step Kit as a wet mix (purple bars) or reconstituted mix after lyophilization (blue bars) in singleplex. SARS-CoV-2 targets used were the N gene (CDC N1 and N2 sequences), E gene ( WHO/CDC sequences), and RdRp gene ( WHO sequences). Ct values are shown in the top panels and efficiency in the bottom panels. 5 serial dilutions of RNA template were used corresponding to 40000, 4000, 400, 40, and 4 copies of the SARS-CoV-2 genome. The total reaction volume was 20 μL. Cycle conditions were 55 °C 5 min, 95 °C 3 min and 50 cycles of 95°C 15 s, 58 °C 30 s. Lyo-Ready Probe 1-Step Kit shows no loss of efficiency or sensitivity after lyophilization.
주문정보
Cat. No. | 품명 | 규격 | 제조사 | 비고 |
PB90.02-03 | Lyo-Ready Probe Mix | 600 x 20 μL Reactions | PCRbiosystems |
4x Lyo-Ready Probe Mix (3 x 1 mL) |
PB90.02-10 | Lyo-Ready Probe Mix | 2,000 x 20 μL Reactions (2 x 5 mL) |
PCRbiosystems |
4x Lyo-Ready Probe Mix (2 x 5 mL) |
PB90.02-50 | Lyo-Ready Probe Mix | 10,000 x 20 μL Reactions (1 x 50 mL) |
PCRbiosystems | 4x Lyo-Ready Probe Mix (1 x 50 mL) |
PB90.02-500 | Lyo-Ready Probe Mix | 100,000 x 20 μL Reactions (1 x 500 mL) |
PCRbiosystems | 4x Lyo-Ready Probe Mix (1 x 500 mL) |
PB90.13-03 | Pre-Lyo Probe 1-Step Evaluation Kit | 600 x 20 μL Reactions | PCRbiosystems | 4x Lyo-Ready Probe Mix (3 x 1 mL) 20x UltraScript RTase (1 x 600μL) |
PB90.14-10 | Lyo-Ready Probe 1-Step Kit | 2,000 x 20 μL Reactions (2 x 5 mL) |
PCRbiosystems | 4x Lyo-Ready Probe Mix (2 x 5 mL) 3,200x UltraScript RTase (1 x 12.5 μL) |
PB90.14-50 | Lyo-Ready Probe 1-Step Kit | 10,000 x 20 μL Reactions (1 x 50 mL) |
PCRbiosystems | 4x Lyo-Ready Probe Mix (1 x 50 mL) 3,200x UltraScript RTase (1 x 62.5 μL) |
PB90.14-500 | Lyo-Ready Probe 1-Step Kit | 100,000 x 20 μL Reactions (1 x 500 mL) |
PCRbiosystems | 4x Lyo-Ready Probe Mix (1 x 500 mL) 3,200x UltraScript RTase (1 x 625 μL) |
관련 논문
.
Document
제품 자료 | Lyo-Ready-Probe-1-Step-Kit-Flyer.pdf | Upload date: 2022.05.31 |
제품 매뉴얼 | PB90.02-Lyo-Ready-Probe-Mix-v1.pdf PB90.13-Pre-Lyo-Probe-1-Step-Evaluation-Kit-v1.pdf PB90.14-Lyo-Ready-Probe-1-Step-Kit-v1.pdf |
Upload date: 2022.05.31 |
제품 MSDS | PB90-Lyo-Ready-Probe-Mix-and-1-Step-Kit-MSDS.pdf | Upload date: 2022.05.31 |