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IsoFast Bst Polymerase & Mix

제품 소개

빠르고 민감한 isothermal amplification 을 위한 새로운 시약인 IsoFast™ Bst Polymerase 가 새롭게 출시되었습니다.
IsoFast™ Bst Polymerase는 DNA 가닥을 ‘unzip’ 하는 고유의 기능을 통해 별도의 전문적인 thermal cycler가 없어도 일반적으로 30분 이내로 실험 결과를 도출할 수 있습니다.
PCR Biosystems의 IsoFast ™ Bst 제품군은 65 ° C의 일정한 온도에서 DNA 및 RNA target의 빠르고 세심한 증폭을 제공합니다.

제품특징

• SARS-CoV-2 의 정확한 검출
>N gene and E gene tested
• DNA와 RNA 검출을 위한 편리한 2x mix 제공
>Save time in set up
>High throughput applications
• Bst enzyme available separately
>Increased flexibility with set up
• 미리 혼합된 RNase inhibitor
> RNase 오염으로 RNA degradation 방지
• 폭넓은 dynamic range에 대한 보다 빠른 검출
> 15분~20분 내에 검출
> Sensitivities down to 3 copies of target DNA (10fg) and 12 copies of SARS-CoV-2 targets
• 동결건조 가능한 Glycerol-free enzyme

응용분야

• LAMP 분자진단
• 현장진단 (POCT) 키트 제조
• 동물 질병 진단
• 식물 질병 진단
• 식품시험
• 바이러스 검사- TB, West Nile, Ebola, Zika, Yellow fever
• SARS-CoV-2 testing groups, health trusts, 컨소시엄

보관온도

• -20℃

Figure 1. Active over a broad temperature range
Isothermal amplification of scaffolding protein gene using M13 genome was successfully achieved incubating the reaction between 55˚C and 70.1˚C.

Figure 2. Flexible formats providing consistent results
No difference in isothermal amplification of scaffolding protein gene using M13 genome was observed using either 2x IsoFast Bst Mix or 10x IsoFast Bst Polymerase.

Figure 3. Rapid and consistent amplification performance across a range of templates
Isothermal amplification of 3 targets using IsoFast™ Bst Mix, NEB WarmStart® LAMP Kit, and NEB Bst 2.0 DNA Polymerase. The manufacturers’ protocols were followed to set up the reaction mix. The primer mix consists of 0.2µM for F3 and B3 primers, 1.6µM for FIP and BIP primers, and 0.8µM for LoopF and LoopB primers. The total reaction volume is 25µL. 7 serial dilutions of template were used (8 for scaffolding protein gene), corresponding to the copy number indicated. The reaction was run at 65°C for 100 minutes. A BioRad CFX96 Touch instrument was used to record fluorescence every 10 seconds. The time to result is the time required to reach the same fluorescent threshold. IsoFast™ Bst Mix shows fast amplification and consistent performance across a range of templates when compared to leading manufacturers.

주문정보

제품번호 품명 규격 제조사 비고
PB80.10-01 IsoFast Bst Polymerase 1,600 units PCRBIOsystems [1 x 200μL 8U/μL] & [1 x 500μL Buffer A] & [1 x 1mL Buffer B]
PB80.10-08 IsoFast Bst Polymerase 8,000 units PCRBIOsystems [1 x 1mL 8U/μL] & [2 x 1.25mL Buffer A] & [3 x 1.7mL Buffer B]
PB80.11-01 IsoFast Bst Polymerase with Dye 1,600 units PCRBIOsystems [1 x 200μL 8U/μL] & [1 x 500μL Buffer A] & [1 x 1mL Buffer B] & [2 x125μL Dye]
PB80.11-08 IsoFast Bst Polymerase with Dye 8,000 units PCRBIOsystems [1 x 1mL 8U/μL] & [2 x 1.25mL Buffer A] & [3 x 1.7mL Buffer B] & [2 x625μL Dye]
PB80.12-01 IsoFast Bst Mix 100 rxns PCRBIOsystems [1 x 1.25mL Mix] & [1 x 125μL Dye]
PB80.12-05 IsoFast Bst Mix 500 rxns PCRBIOsystems [5 x 1.25mL Mix] & [1 x 625μL Dye]
PB80.30-02 Fluorescent Dye 200 rxns PCRBIOsystems 1 x 125μL
PB80.30-10 Fluorescent Dye 1,000 rxns PCRBIOsystems 1 x 625μL

Document

제품 자료 IsoFast Bst Polymerase Flyer.pdf Upload Data : 2021.06.21
제품 매뉴얼 PB80.10 IsoFast Bst Polymerase Manual.pdf Upload Data : 2021.06.21
PB80.11 IsoFast Bst Polymerase with Dye Manual.pdf Upload Data : 2021.06.21
PB80.12 IsoFast Bst Mix Manual.pdf Upload Data : 2021.06.21
PB80.30 Fluorescent Dye Manual.pdf Upload Data : 2021.06.21
제품 MSDS PB80.10 IsoFast Bst Polymerase MSDS.pdf Upload Data : 2021.06.21
PB80.11 IsoFast Bst Polymerase with Dye MSDS.pdf Upload Data : 2021.06.21
PB80.12 IsoFast Bst Mix MSDS.pdf Upload Data : 2021.06.21
PB80.30 Fluorescent Dye MSDS.pdf Upload Data : 2021.06.21