Project Description

IsoFast™ Bst 1-Step Mix

제품 소개

IsoFast™ Bst 1-Step Mix는 RNA target의 빠르고 민감한 isothermal amplification 을 위한 이중 효소 시스템입니다.
kit에는 IsoFast™ Bst Polymerase가 포함되어 있어 강력한  성능을 제공하며, 고활성 MLV Rtase Go도 포함되어 있습니다

제품특징

• SARS-CoV-2  핵산의 정량 검출
• 2x mix IsoFast™ Bst Polymerase 포함
• RTase Go plus RNase inhibitor 제공
• 65˚C 에서 반응 수행
• 다양한 템플릿에서 빠르고 일관된 증폭을 제공
• 까다로운 조건에서 더 높은 수율을 위한 버퍼 시스템 포함
• fluorescent dye for real-time detection을 휘한 fluorescent dye 제공
• 30 minute protocol

응용분야

• LAMP 분자진단
• 현장진단 (POCT) 키트 제조
• 동물 질병 진단
• 식물 질병 진단
• 식품시험
• 바이러스 검사- TB, West Nile, Ebola, Zika, Yellow fever
• SARS-CoV-2 testing groups, health trusts, 컨소시엄

보관온도

• -20℃

Figure 1. Rapid and sensitive amplification performance
Isothermal amplification of beta actin from human lung total RNA using IsoFast™ Bst 1-Step Mix. A primer mix of 0.2µM for F3 and B3 primers, 1.6µM for FIP and BIP primers, and 0.8µM for LoopF and LoopB primers were used. The total reaction volume was 25µL. 7 serial dilutions of template were used, corresponding to 250ng, 25ng, 2.5ng, 250pg, 25pg, 2.5pg, and 250fg of total RNA. The reaction was run at 65˚C for 34 minutes. A BioRad CFX96 Touch instrument was used to record fluorescence every 10 seconds. Time to result is the time required to reach the same fluorescent threshold. IsoFast™ Bst 1-Step Mix provides rapid and sensitive amplification down to 2.5pg of total RNA.

Figure 2. Rapid detection of SARS-CoV-2 E1 and N2 sequence
Isothermal amplification of E1 and N2 targets in SARS-CoV-2 RNA using IsoFast™ Bst 1-Step Mix and compared to results obtained with NEB WarmStart® LAMP Kit. A primer mix of 0.2µM for F3 and B3, 1.6µM for FIP and BIP and 0.8µM for LoopF and LoopB primers was used. The total reaction volume was 25µL. 7 serial dilutions of template were used, corresponding to 40,000, 8000, 1600, 320, 64, 12.8, 2.56 copies of SARS-CoV-2 RNA. The reaction was run at 65˚C for 100 minutes. A BioRad CFX96 Touch instrument was used to record fluorescence every 10 seconds. Time to result is the time required to reach the same fluorescent threshold. IsoFast™ Bst 1-Step Mix enables rapid detection of all dilutions of SARS-CoV-2 RNA tested.

주문정보

제품번호 품명 규격 제조사 비고
PB80.21-01 IsoFast™ Bst 1-Step Mix 100 rxns PCRBIOsystems [1 x 1.25mL Bst Mix] & [1 x 200μL RTase Go] & [1 x 125μL Dye]
PB80.21-05 IsoFast™ Bst 1-Step Mix 500 rxns PCRBIOsystems [4 x 6.25mL Bst Mix] & [1 x 1mL RTase Go] & [1 x 625μL Dye]

Document

제품 자료 IsoFast Bst 1-Step Mix Flyer.pdf Upload Data : 2021.06.21
제품 매뉴얼 PB80.21 IsoFast Bst 1-Step Mix Manual.pdf Upload Data : 2021.06.21
제품 MSDS PB80.21 IsoFast Bst 1-Step Mix MSDS.pdf Upload Data : 2021.06.21