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HS Taq DNA Polymerase and Mix

제품 소개

PCRBIO HS Taq DNA Polymerase는 항체 매개 hot start 기술로, target gene에 대한 특이성을 높인 제품입니다.
DNA 중합 효소는 95℃의 초기 활성 단계까지 억제되어 프라이머 dimer 형성 및 비특이적 증폭을 효과적으로 저해합니다.
또한 효소 촉매반응부위의 최적화 과정을 거쳐 GC, AT 비율이 높은 복잡한 template의 증폭에도 효과적입니다.

제품특징

• primer dimer 및 비특이적 증폭을 저해하는 hot-start 기술
• Amplification size: < 6 kb
• 5’→3’ exonuclease activity
• 안정성: 37℃에서 4주간 안정

응용분야

• Genotyping
• Difficult PCR – GC/AT rich DNA
• Routine and multiplex PCR
• Colony PCR
• TA Cloning

보관온도

• -20℃
• Stable for 4weeks at 37℃

Figure 1. Shows amplification of a 1kb fragment of Beta-Actin under standard cycling conditions. The top row is PCRBIO HS Taq DNA Polymerase and the 2nd row is an equivalent product from K Comapny.

Figure 2. Shows no change in activity is detected in PCRBIO HS Taq Mix Red after 28 days at 4˚C, 25˚C and 37

Figure 2B. Shows amplification of a 400bp gene from human genomic DNA under fast cycling conditions. The top row is PCRBIO HS Taq DNA Polymerase, the 2nd row is the equivalent product from K company and the 3rd row is the equivalent product from I comapny.

주문정보

제품번호 품명 규격 제조사 비고
PB10.21-05 PCRBIO HS Taq DNA Polymerase 250 units PCRbiosystems [1 x 50ul 5 units/μl] & [2 x 1ml buffer]
PB10.21-10 PCRBIO HS Taq DNA Polymerase 1,000 units [4 x 50ul 5 units/μl] & [8 x 1ml buffer]
PB10.22-02 PCRBIO HS Taq Mix 200 rxns 5 x 1ml
PB10.22-10 PCRBIO HS Taq Mix 1,000 rxns 25 x 1ml
PB10.23-02 PCRBIO HS Taq Mix Red 200 rxns 5 x 1ml
PB10.23-10 PCRBIO HS Taq Mix Red 1,000 rxns 25 x 1ml
PB10.23-96 PCRBIO HS Taq PreMix 96 rxns 96 tubes
PB10.23-480 PCRBIO HS Taq PreMix 480 rxns 96 tubes x 5

관련 논문

PCRBIO HS Taq DNA Polymerase 

Montagnier, L., Aïssa, J., Capolupo, A., Craddock, T.J.A., Kurian, P., Lavallee, C., Polcari, A., Romano, P., Tedeschi, A. and Vitiello, G., 2017. Water Bridging Dynamics of Polymerase Chain Reaction in the Gauge Theory Paradigm of Quantum Fields. Water9(5), p.339.

Nusová, G., Šemeláková, M., Paučulová, L., Uhrin, M. and Kaňuch, P., 2017. Haplotype diversity in common pipistrelle’s mass hibernacula from central Europe. Biologia72(5), pp.548-553.


PCRBIO HS Taq Mix
 

​Borgbo, T., Chrudimska, J., Macek, M., Jeppesen, J.V., Bøtkjær, J.A., Kristensen, S.G., Macklon, K.T., Ernst, E., Hansen, L.L. and Andersen, C.Y., 2017. The polymorphic insertion of the luteinizing hormone receptor “insLQ” show a negative association to LHR gene expression and to the follicular fluid hormonal profile in human small antral follicles. Molecular and Cellular Endocrinology.

Chung, S.W., Choi, B.M., Kim, J.Y., Lee, Y.S., Yoon, J.P., Oh, K.S. and Park, K.S., 2017. Altered Gene and Protein Expressions in Torn Rotator Cuff Tendon Tissues in Diabetic Patients. Arthroscopy: The Journal of Arthroscopic & Related Surgery33(3), pp.518-526.

Hong, S.H., Kim, J.S., Park, J.W. and Lee, E.Y., 2015. A Study on the Effect of the RhizobacteriumBacillus sp. SH1RP8 and Potassium Family Polymers on the Crop Growth under Saline. Korean Society for Biotechnology and Bioengineering Journal. 30(3): 97-102.

Matějčková, I., 2017. Utilization of New Generation Sequencing Methods to Elucidate Cystic FibrosisLike Phenotype at Patients with Unclear Illness of Molecular Type. (Univerzita Karlova).

Tap, R.M., Betty, H., Sue, L., Ramli, N.Y., Suppiah, J., Hashim, R., Sabaratnam, P., Ginsapu, S.J., Gowbei, A., Razak, M.F.A. and Sipiczki, M., 2016. First isolation of Candida wangnamkhiaoensis from the blood of immunocompromised paediatric patient. Mycoses59(11), pp.734-741.


PCRBIO HS Taq Mix Red  

Ingrao, A.J., Schmidt, J., Jubenville, J., Grode, A., Komondy, L., VanderZee, D. and Szendrei, Z., 2017. Biocontrol on the edge: Field margin habitats in asparagus fields influence natural enemy-pest interactions. Agriculture, Ecosystems & Environment243, pp.47-54.

Naďo, L., Chromá, R. and Kaňuch, P., 2017. Structural, temporal and genetic properties of social groups in the short-lived migratory bat Nyctalus leisleriBrill

Schmidt, J.M., Szendrei, Z. and Grieshop, M., 2016. Elucidating the Common Generalist Predators of Conotrachelus nenuphar (Herbst)(Coleoptera: Curculionidae) in an Organic Apple Orchard Using Molecular Gut-Content Analysis. Insects7(3), p.29.

Zahradnik, J., Kolenko, P., Cerny, J., Palyzova, A., Kolarova, L., Kyslikova, E., Maresova, H., Grulich, M, Kyslik, P. and Schneider, B., 2016. A crystal structure of an Apo form of glycerol trinitrate reductase XdpB, a member of a subgroup of bacterial Old Yellow Enzymes with autogenic blocking, from Agrobacterium sp. R89-1. (Univerzita Karlova v Praze).

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제품 자료 PCRBIO-HS-Taq-DNA-Polymerase-Flyer Download Size : 604kB
PCRBIO-HS-Taq-Mix-Red-Flyer Download Size : 680kB
제품 MSDS PCRBIO-HS-Taq-DNA-Polymerase-MSDS Download Size : 289kB
PCRBIO-HS-Taq-Mixes-MSDS Download Size : 246kB
제품 매뉴얼 PCRBIO-HS-Taq-DNA-Polymerase-manual Download Size : 61kB
PCRBIO-HS-Taq-Mix-Red-manual Download Size : 61kB