Project Description

qPCRBIO HRM Mix

제품 소개

High Resolution Melt 분석은 이중가닥의 DNA 샘플에서 mutation, SNP genotyping이나 methylation 분석 등을 할 수 있는 기술입니다.
qPCRBIO HRM Mix는 Hot start polymerase, dNTPs, buffer, Mgcl2와 기존 SyGreen보다 double strand 상태의 DNA에 대한 감도가 높은 진보된 SyGreen II dye를 혼합해 높은 형태로, HRM 기능이 있는 thermal cycler에서 사용이 가능한 제품입니다.

제품특징

• 3세대 saturating dye SyGreen II 혼합
• Antibody-mediated hot start

응용분야

• SNP genotyping
• Gene scanning
• CpG methylation analysis

보관온도

•-20℃

HRM analysis of class I, II, III and IV single nucleotide polymorphisms. In each case, three samples of human genomic DNA were amplified using qPCRBIO HRM Mix with primers specific for a fragment of gene containing the SNPs. Figure 1 shows Factor V 1691G/A (class I), figure 2 shows MTHFR 1298A/C (class II) After amplification the products were subjected to HRM analysis. The traces show clear allele calling of class I, II, III and IV SNPs.

HRM analysis of class I, II, III and IV single nucleotide polymorphisms. In each case, three samples of human genomic DNA were amplified using qPCRBIO HRM Mix with primers specific for a fragment of gene containing the SNPs. figure 3 shows HFE 187 C/G (class III) and figure 4 shows beta-globulin 17A/T (class IV). After amplification the products were subjected to HRM analysis. The traces show clear allele calling of class I, II, III and IV SNPs.

주문정보

Cat. No. 품명 규격 제조사 비고
PB20.31-01 qPCRBIO HRM Mix 100 rxns PCRbiosystems 1 x 1mL
PB20.31-05 qPCRBIO HRM Mix 500 rxns PCRbiosystems 5 x 1mL

관련 논문

qPCRBIO HRM Mix

Yang, J., Kemps-Mols, B., Spruyt-Gerritse, M., Anholts, J., Claas, F. and Eikmans, M., 2016. The source of SYBR green master mix determines outcome of nucleic acid amplification reactions. BMC research notes9(1), p.292.

Document

제품 자료 Upload date: 2021.07.13
제품 매뉴얼 PB20.31-qPCRBIO-HRM-Mix-Manual.pdf Upload date: 2021.07.13
제품 MSDS PB20-qPCRBIO-Real-Time-Mixes-MSDS-1.pdf Upload date: 2021.07.13