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PCR DIRECT Extracting Transport Medium

제품 소개

PCR DIRECT Extracting Transport Medium(ETM)은 면봉 또는 타액 샘플 키트와 함께를 사용하여, 채취시점에 바이러스를 비활성화 하고 숙주 핵산(DNA 및 RNA)의 추출 안정성을 도모하기 위해 사용하는 제품입니다.

이 제품은 낮은 비용으로 증가된 감도를 제공하며, 추출된 핵산은 direct PCR/RT-qPCR 이나 bead/column-based kits를 사용한 후속 작업에 사용할 수 있습니다

Arcis의 PCR DIRECT 시약은 신속한 용해, 핵산 보호 및 잠재적인 PCR inhibitors의 차단을 위해 독점적인 One-Step™ chemistry 을 사용합니다.
면봉 또는 타액 샘플을 초고속으로 처리할 수 있으므로 연구자는 1번의 피펫팅으로도 direct PCR/RT-qPCR에 적합한 높은 감도와 재현성을 가진 결과물을 빠르게 얻을 수 있습니다.

제품특징

• 유해하지 않은 화학 물질로 폐기 비용 없음
• 상온에서 핵산 추출 수행-비용 절감
• 다른 장비나 시약 없이도 충분히 높은 감도 보장
• PCR inhibitors 불활성화
• 실온에서 최대 4 일간 RNA 안정

PCR DIRECT ETM’s non-hazardous lysis formulation delivers superior sensitivity in direct RT-qPCR and improves sensitivity when used with isolation kits

Swab samples extracted with PCR DIRECT ETM are compatible with Direct RT-qPCR or isolation kits. Contrived SARS-CoV-2 samples were generated by collecting swabs and spiking with heat-inactivated SARS-CoV-2. The spiked swabs were placed in ETM or Saline/PBS and tested directly by RT-qPCR or after processing with a bead-based and a column-based RNA isolation kit. ETM effectively lysed the virus and delivered improved sensitivity when paired with a bead-based RNA isolation. Equivalent sensitivity was observed with a column-based isolation kit.

Saliva extracted with PCR DIRECT ETM is compatible with Direct RT-qPCR or isolation kits. Contrived SARS-CoV-2 samples were generated by collecting saliva by active spitting and spiking with heat-inactivated SARS-CoV-2. The spiked saliva was mixed with ETM or Saline/PBS and tested directly by RT-qPCR (using Thermo Fisher Taqman Fast Virus 1-Step Master Mix) or after processing with a bead-based and a column-based RNA isolation kit. In all cases, ETM effectively liquified the saliva, lysed the virus, and delivered improved sensitivity when paired to both bead- and column-based RNA isolation.

제품사양

UFL101 UFL105
Sample type Swabs (oral, nasal, nasopharyngeal), Saliva Swabs (oral, nasal, nasopharyngeal), Saliva
Sample input volume Varies Varies
Reaction time Varies Varies
No. of reactions 50 500
Storage Store at room temperature, do not freeze Store at room temperature, do not freeze
Shipping conditions Room temperature Room temperature

프로토콜

Swab Protocol

Swabs, immediately after collection, should be placed into sterile collection tubes containing aliquoted PCR DIRECT ETM.
We recommend preparing sample collection tubes ahead with 300 µl of PCR DIRECT ETM per tube.
For maximum sensitivity in direct RT-qPCR PCR, the reaction may be prepared with up to 50% sample by volume (e.g., up to 12.5 µl sample in a 25 µl reaction).
If a bead- or column-based isolation kit will be used, 200-300 µl of sample in PCR DIRECT ETM may be used for subsequent processing.

  1. Collect oral or nasal swabs according to laboratory procedure.
  2. Place swab head into PCR DIRECT ETM; swirl and rotate swab head against the tube wall 10 times to extract the maximum amount of liquid from the swab head.
  3. Begin to remove the swab, press the swab head against the side of the collection tube to maximize the amount of prepared lysate.
  4. Shake the tube vigorously at least ten times.
  5. A sample is ready for transport and subsequent downstream molecular analysis or processing.

Note: the head of the swab should be fully submerged in a minimal volume of PCR DIRECT ETM. Complete immersion will ensure that the entire collected sample has been lysed. Minimizing the volume of PCR DIRECT ETM ensures the highest concentration of nucleic acids and supports direct PCR testing methods.

Saliva Protocol

For saliva samples, donors should be instructed not to consume food or drink, or use gum, toothpaste for at least 30 minutes prior to sample collection.
Saliva should not be mixed with other transport media or lysis reagents prior to the addition of the PCR DIRECT ETM as this may affect results.
This protocol may be adjusted for larger sample volumes by scaling the volume of PCR DIRECT ETM in proportion to the increase in the sample volume.
If a bead- or column-based isolation kit will be used, 200-400µl of sample in PCR DIRECT ETM may be used for subsequent processing.

  1. Add 100 µl of saliva to a 1.5 ml low binding microtube.
  2. Add 235 µl of PCR DIRECT ETM to the sample with the same pipette, mix ten (10) times.
  3. Sample is ready for transport and subsequent downstream molecular analysis or processing.

Note: Inadequate mixing in step 2 will result in lower sensitivity. Mixing by pipetting is preferred.

Compare PCR DIRECT Extracting Transport Medium

Combines swab or saliva sample transport, viral inactivation without guanidinium, and RNA extraction in transit, while delivering increased sensitivity at a lower cost.

PCR Direct
Extracting Transport Medium
Traditional Transport Media
(Contains Guanidinium)
Traditional Inactivation Media
(UTM/VTM)
Cold chain storage and transport required? No No Yes
Viruses inactivated? Yes
(> 4 log reduction with Corona 229E)
Yes. Can test outside containment No. Must test under containment
Contains guanidinium? No Yes No
Environmentally safe? Yes (safe with bleach and acids) No (can lead to serious injury with bleach and acids) Yes
The integrity of nucleic acids in the sample? Preserves RNA and DNA without denaturing enzymes Preserves RNA and DNA but damages enzymes It May contain enzymes that damage RNA and DNA
Enables direct testing from the sample? Yes
LOD swab: 900 cps/sample
LOD saliva: 4200 cps/ml
No No
Reduces viscosity to enable automation? Yes No No

PCR Direct Guide to Product Selection

Arcis PCR DIRECT® Product Swab Extraction Reagent Saliva Extraction Reagent Extracting Transport Medium
For use in the Lab O O
For use at the point of collection* O
Extract nucleic acids from dry samples. Avoid the need for media O O
Simplify protocol:
no incubation, no capture, and concentration save time and money
O O O
Reduce viscosity of samples, enable automation O O O
Achieve higher sensitivity compared to other direct methods O O O
Avoid guanidinium & hazardous waste O O O
Achieve greatest possible safety through earliest inactivation O
Sequesters RNases O O O
Sample Type Swab Saliva Swab/Saliva

*Apply specified volume to sample with dropper or pre-aliquot into a collection device.

주문정보

​ 제품번호 품명 규격 제조사 비고
UFL101 PCR DIRECT Extracting Transport Medium(50 Preps) 50 rxns Arcis Biotechnology
UFL105 PCR DIRECT Extracting Transport Medium(500 Preps) 500 runs Arcis Biotechnology

Document

제품 자료
제품 MSDS MSDS-Arcis-Virus-Sample-Prep-Reagent.pdf  Upload date: 2022.02.23
제품 매뉴얼 PCR-DIRECT-ETM-UFL101-v3.pdf
PCR-DIRECT-ETM-UFL105-v2.pdf
Upload date: 2022.02.23