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Claro™ Sola ECL solution

제품 소개

Claro ECL solution은 Western blot을 위한 detection reagent로, 낮은 antibody 농도에서도 높은 감광을 보입니다.
Solution A (Luminol/enhancer)와 Solution B (Peroxide)를 각각 1:1의 비율로 혼합하여 사용합니다.
민감도에 따라 3가지 제품군으로 나뉘어 있어, 사용자의 목적에 맞게 선택이 가능합니다.

Claro Sola ECL solution은 low-picogram 검출 레벨에 안정적인 광 출력을 갖는 ECL substrate입니다.
이 제품은 높은 signal에서 noise ratio에 대한 낮은 background를 제공 합니다.


• 고감도
• Quantitative: linear dynamic range
• 긴 신호 지속 시간
• Solution A: Luminol/enhancer solution (amber bottle) / Solution B: Peroxide solution (white bottle)
• Low-picogram detection
• 3일 동안 안정한 작업 용액


• Routine protein detection
• Protein confirmation
• Overexpressed proteins
• Tagged proteins


• Storage at room temperature 1 year

Hela whole cell lysate (Abcam ® ) was diluted to 250 μg/mL in electrophoresis reducing sample buffer and  1:1  serial dilutions were made.  5μL  of each dilution (1250 ng to 78 ng of total protein) were separated by SDS-PAGE  and the proteins were transferred to  Amersham™  Protran ®   Premium  0,45  NC  membrane (GEHC). The membrane was blocked with 5% non-fat dry milk in  1X  TBS  TWEEN ® 20  Buffer.  Then the membrane was incubated with  Rabbit Anti-MAP Kinase (ERK 1/2) antibody (SIGMA) at 1:1500 followed by incubation with Anti-Rabbit IgG HRP Linked F(ab′)2 (GEHC) at 1:20000.


Cat. No. 품명 규격 제조사 Antibody Dilution Range
HQS071, 0250 Claro Sola ECL Solution
-Low picogram
2 x 125mL BioD Primary: 1:500 – 1:5,000
Secondary: 1:20,000 – 1:100,000
HQS070, 0250 Claro Mucho ECL Solution
-Mid femtogram
2 x 125mL BioD Primary: 1:1,000 – 1:15,000
Secondary: 1:25,000 – 1:150,000
HQS075, 0100 Claro Supremo ECL Solution
-Mid femtogram to  low femtogram
2 x 50mL BioD Primary: 1:5,000 – 1:50,000
Secondary: 1:50,000 – 1:250,000


제품 자료 BioD-Claro-ECL-solution.pdf Upload date: 2021.07.14
제품 매뉴얼 Manual_Sola ECL.pdf Upload date: 2021.07.14
제품 MSDS MSDS-Sola-Luminol.pdf
Upload date: 2021.07.14