Figure 1. Hela whole cell lysate (Abcam ® ) was diluted to 250 μg/mL in electrophoresis reducing sample buffer and 1:1 serial dilutions were made. 5μL of each dilution (1250 ng to 78 ng of total protein) were separated by SDS-PAGE and the proteins were transferred to Amersham™ Protran ® Premium 0,45 NC membrane (GEHC). The membrane was blocked with 5% non-fat dry milk in 1X TBS TWEEN ® 20 Buffer. Then the membrane was incubated with Rabbit Anti-MAP Kinase (ERK 1/2) antibody (SIGMA) at 1:2000 followed by incubation with Anti-Rabbit IgG HRP Linked F(ab′)2 (GEHC) at 1:25000.