BlotCycler Mini Automated Western Blot

기존에 사용하던 소모품 (buffer, membrane, antibody 등)을 그대로 사용 가능합니다. 2개의 tray 장착으로 2종류의 항체로 최대 8개 Blot을 동시에 Processing

Collection tube 를 통해 실험 후 Primary antibody를 회수하여 다시 사용할 수 있습니다.

Protocol editor를 통해서 Blocking, Antibody Incubation, Washing 각 단계의 시간 설정, 반복 횟수, 등을 간편한 세팅하여 저장할 수 있습니다.

Versatility
BlotCycler™ is compatible with multiple immunodetection technologies including fluorescent and chemiluminescent detection. In the examples shown at right, blots were processed using the same protocol using BlotCycler™ (left) or manually (right). Fluorescence labeled secondary antibodies were used to detect proteins. (Thanks to The Aaron Diamond AIDS Research Center, NY.)

Reproducibility
Five identical blots were processed using BlotCycler™. Each blot contained three lanes: HeLa whole cell lysate; the recombinant protein Human IL-2, and the mixture of HeLa whole cell lysate and h-IL-2. Blots were incubated with a mix of rabbit anti-IL2 and mouse anti-α-tubulin, then probed with corresponding secondary antibodies: DyLight™ 549 conjugated anti-mouse IgG and DyLight™ 649 conjugated anti-rabbit IgG.