Scicons Anti dsRNA Monoclonal Antibody

제품 소개

이중 가닥 RNA(dsRNA)는 모든 세포에서 발견되는 DNA와 유사하지만 티민이 우라실로 대체되고 하나의 산소 원자가 추가된 두 개의 상보적 가닥이 있는 RNA입니다.
dsRNA는 일부 바이러스(이중 가닥 RNA 바이러스)의 유전 물질을 형성합니다.
바이러스 RNA 또는 siRNA와 같은 이중 가닥 RNA는 척추동물에서 인터페론 반응뿐만 아니라 진핵생물에서 RNA interference 를 유발할 수 있습니다.

Scicons 사는 이중 가닥 RNA 분자에 결합하는 4개의 마우스 단일클론항체와 이를 기반으로 한 ELISA  kit를 제조합니다.
Anti-dsRNA antibody는 미지의 병원체가 본질적으로 박테리아인지 바이러스인지를 검출하는 진단 도구로 사용될 수 있습니다.

It can be used to detect dsRNA intermediates of viruses as diverse as Hepatitis C virus, Dengue virus, rhinovirus, Chikungunya virus, Rabies virus, Poliovirus, Classic swine fever virus, Brome mosaic virus, and many more in cultured cells and also in fixed paraffin-embedded histological samples.

Related Brand:
Scicons, Nordic Biosite, Exalpha, Nordic, Gallus, MUbio, Biologo, ADG, ADG (CE/IVD), ELTI, MUbio (CE/IVD), Bii.

1) F. Weber, V. Wagner, S. B. Rasmussen, R. Hartmann, S. R. Paludan. Double-stranded RNA is produced by positive-strand RNA viruses and DNA viruses but not in detectable amounts by negative-strand RNA viruses. J Virol (2006), 80(10):5059-64. doi: 10.1128/JVI.80.10.5059-5064.2006.

2) S. Welsch, S. Miller, I. Romero-Brey, A. Merz, C. K. E. Bleck, P. Walther, S. D. Fuller, C. Antony, J. Krijnse-Locker, R. Bartenschlager. Composition and Three-Dimensional Architecture of the Dengue Virus Replication and Assembly Sites. Cell Host & Microbe (2009) 5(4); 365-375. doi.org/10.1016/j.chom.2009.03.007.

3) K. Knoops , M. Bárcena, R. W. Limpens, A. J. Koster, A. M. Mommaas, E. J. Snijder. Ultrastructural characterization of arterivirus replication structures: reshaping the endoplasmic reticulum to accommodate viral RNA synthesis. J Virol. (2012) 86(5); 2474-2487. doi:10.1128/JVI.06677-11.

4) S. J. Richardson, A. Willcox, D. A. Hilton, S. Tauriainen, H. Hyoty, A. J. Bone, A. K. Foulis, N. G. Morgan. Use of antisera directed against dsRNA to detect viral infections in formalin-fixed paraffin-embedded tissue. J Clin Virol. (2010) 49(3); 180-5. doi: 10.1016/j.jcv.2010.07.015.

5) K. Karikó, H. Muramatsu, J. Ludwig, D. Weissman, Generating the optimal mRNA for therapy: HPLC purification eliminates immune activation and improves translation of nucleoside-modified, protein-encoding mRNA, Nucleic Acids Research (2011) 39(21); e142, https://doi.org/10.1093/nar/gkr695.

6) Schönborn, J., Oberstrass, J., Breyel, E., Tittgen, J., Schumacher, J. and Lukacs, N. (1991) Monoclonal antibodies to double-stranded RNA as probes of RNA structure in crude nucleic acid extracts. Nucleic Acids Res.19, 2993-3000.

7) Lukacs, N. (1994) Detection of virus infection in plants and differentiation between coexisting viruses by monoclonal antibodies to double-stranded RNA. J. Virol. Methods 47, 255-272.

8) Lukacs, N. (1997) Detection of sense:antisense duplexes by structurespecific anti-RNA antibodies. In: Antisense Technology. A Practical Approach, C. Lichtenstein and W. Nellen (eds), pp. 281-295. IRL Press, Oxford.

Recent publication:
Tirosh Shapira, I. Abrrey Monreal, Sébastien P Dion, Mason Jager, Antoine Désilets, Andrea D Olmstead, Thierry Vandal, David W Buchholz, Brian Imbiakha, Guang Gao, Aaleigha Chin, William D Rees, Theodore Steiner, Ivan Robert Nabi, Eric Marsault, Julie Sahler, Avery August, Gerlinde Van de Walle, Gary R Whittaker, Pierre-Luc Boudreault, Hector C Aguilar, Richard Leduc, François Jean. A novel highly potent inhibitor of TMPRSS2-like proteases blocks SARS-CoV-2 variants of concern and is broadly protective against infection and mortality in mice. bioRxiv 2021.05.03.442520; doi: https://doi.org/10.1101/2021.05.03.442520 https://biorxiv.org/cgi/content/short/2021.05.03.442520v1