Air Dryable Probe 1 Step Mix

Fig 1. Reliable performance after air-drying in a multiplex setup.
Multiplex amplification of Influenza A, Influenza B, Respiratory Syncytial Virus, and SARS-CoV-2 using Air-Dryable Probe 1-Step Mix before (blue bars) or after drying (purple bars). Ct values are shown in the left panels and efficiency in the right panels. 5 serial dilutions of RNA template were used, corresponding to 40 000, 4 000, 400, 40, and 4 copies of each viral genome. The total reaction volume was 20 µL. Cycle conditions were 45 °C for 20 min, 95 °C 3 min and 50 cycles of 95 °C 15 s, 60 °C 30 s.

Fig 2. Stability of the dried gel after 12 weeks at 37 °C
Amplification of common house-keeping genes (β-Actin, γ-Actin) in singleplex setup using Air-Dryable Probe 1-Step Mix. The mix was dried and stored at -20 °C (blue curves) or incubated at 37 °C for 12 weeks (purple curves). Amplification curves are shown in the left panels and efficiency in the right panels. 3 serial dilutions of mouse total RNA template were used, corresponding to 5 ng/µL, 500 pg/µL, and 50 pg/µL. The total reaction volume was 20 µL. Cycle conditions were 45 °C 20 min, 95 °C 3 min and 50 cycles of 95 °C 15 s, 58 °C 30 s.
Air-Dryable Probe 1-Step Mix shows no significant loss of efficiency (less than 10% difference), speed (less than 1 Ct delay) or sensitivity after incubation at 37 °C for 12 weeks.