Fig 1. Reliable performance after air-drying in a multiplex setup.
Multiplex amplification of Influenza A, Influenza B, Respiratory Syncytial Virus, and SARS-CoV-2 using Air-Dryable Probe 1-Step Mix before (blue bars) or after drying (purple bars). Ct values are shown in the left panels and efficiency in the right panels. 5 serial dilutions of RNA template were used, corresponding to 40 000, 4 000, 400, 40, and 4 copies of each viral genome. The total reaction volume was 20 µL. Cycle conditions were 45 °C for 20 min, 95 °C 3 min and 50 cycles of 95 °C 15 s, 60 °C 30 s.