Project Description

HOME/Consumable/Real-time PCR Master Mix/Air Dryable Probe 1 Step Mix

Air Dryable Probe 1 Step Mix

제품 소개

최근 분자 진단 테스트 키트의 개발은 활용하기 편하면서 비용 절감도 가능한 동결 건조 및 공기 건조 형식으로 점점 이동하고 있는 추세입니다.
PCRBIOsystems 사에서는 시약 건조의 어려움을 극복하기 위해 글리세롤이 없는 4xqPCR과 보존제를 혼합하여 바로 사용 가능한 형태로 만들었습니다.
기존 qPCR 및 RT-qPCR 시약과 동일한 고성능을 유지하도록 설계된 이 제품들은 상온 보관이 필요한 진단 검사 개발에 매우 이상적입니다.

Key benefits of air-drying

• 시약의 부피와 반응물의 질량 감소로 인해 배송 및 보관 비용이 절감됩니다.
• 공기 건조된 최종 결과물의  상온 안정성으로인해 냉장/냉동 운송 및 보관이 필요 없습니다.
• 물류를 단순화하고 전체 비용을 절감합니다.
• 샘플의 양을 늘려 사용할 수 있습니다. 샘플 입력 볼륨이 최종 반응 볼륨과 동일할 수 있습니다.
• 공기를 통한 자연 건조는 전문 장비 및 지식이 필요하지 않아 접근성이 높은 방법입니다.
• 중소규모 IVD kit  개발자를 위한 최적의 제품입니다.

제품특징

• Unbiased, sensitive detection of both DNA and RNA targets, 4 copies per reaction
• Complete 1-step RT-qPCR mix, single tube format
• Easy reaction setup
• Rapid air-drying protocol, 80-90 min
• Same high performance before & after drying
• Room temperature storage and shipping of dried reactions
• Increased sample volume input

응용분야

• Diagnostic probe-based qPCR & RT-qPCR
• DNA & RNA detection
• TaqMan®, Scorpions® and molecular beacon probe qPCR
• Multiplex and singleplex setup

보관온도

•How do I store my dried reaction mixes?
>Once dried, seal tubes/plates. In this way, they can be stored at room temperature for at least 1.5 years.
>For long-term storage, we recommend storing the plates inside zip lock bags with silica gel bags.

• How should I store the mix before drying?
>between -30 °C and -15 °C. 12 months

• How long is the dried gel stable?
> at 37 °C for 3 months.

Fig 1. Reliable performance after air-drying in a multiplex setup.
Multiplex amplification of Influenza A, Influenza B, Respiratory Syncytial Virus, and SARS-CoV-2 using Air-Dryable Probe 1-Step Mix before (blue bars) or after drying (purple bars). Ct values are shown in the left panels and efficiency in the right panels. 5 serial dilutions of RNA template were used, corresponding to 40 000, 4 000, 400, 40, and 4 copies of each viral genome. The total reaction volume was 20 µL. Cycle conditions were 45 °C for 20 min, 95 °C 3 min and 50 cycles of 95 °C 15 s, 60 °C 30 s.

Fig 2. Stability of the dried gel after 12 weeks at 37 °C
Amplification of common house-keeping genes (β-Actin, γ-Actin) in singleplex setup using Air-Dryable Probe 1-Step Mix. The mix was dried and stored at -20 °C (blue curves) or incubated at 37 °C for 12 weeks (purple curves). Amplification curves are shown in the left panels and efficiency in the right panels. 3 serial dilutions of mouse total RNA template were used, corresponding to 5 ng/µL, 500 pg/µL, and 50 pg/µL. The total reaction volume was 20 µL. Cycle conditions were 45 °C 20 min, 95 °C 3 min and 50 cycles of 95 °C 15 s, 58 °C 30 s.
Air-Dryable Probe 1-Step Mix shows no significant loss of efficiency (less than 10% difference), speed (less than 1 Ct delay) or sensitivity after incubation at 37 °C for 12 weeks.

주문정보

Cat. No. 품명 규격 제조사 비고
PB90.21-03 Air-Dryable Probe 1-Step Mix 600 x 20 μL Reactions PCR Biosystems 3 x 1 mL
PB90.21-10 Air-Dryable Probe 1-Step Mix 2000 x 20 μL Reactions PCR Biosystems 2 x 5 mL
PB90.21-50 Air-Dryable Probe 1-Step Mix 10 000 x 20 μL Reactions PCR Biosystems 1 x 50 mL
PB90.21-500 Air-Dryable Probe 1-Step Mix 100 000 x 20 μL Reactions PCR Biosystems 1 x 500 mL

Document

제품 자료 Air-Dryable-Probe-1-Step-Mix.pdf Upload date: 2022.09.05
제품 매뉴얼 PB90.21-Air-Dryable-Probe-1-Step-Mix_Manual.pdf Upload date: 2022.09.05
제품 MSDS MSDS_PB90.21.pdf Upload date: 2022.09.05