NGS Library Prep QC by Qsep Fragments analyzer

2021-08-04 18:23
Next Generation Sequencing (NGS) Library Prep QC

최근 다양한 분야에서 활용되고 있는 차세대 염기서열 분석(NGS) 과정에서 가장 중요한 과정은 일관적이고 품질 좋은 Library를 분리하는 것이라 할 수 있습니다.

Workflow of Sequencing library construction including many steps of quality control which play an important role to make sure of successful analysis

완전 자동화된 Qsep Bio-Fragment Analyzer(Qsep1, Qsep100 & Qsep400)는
Fragmented DNA/RNA의 Integrity Test는 물론 Selected Fragment Library의 Size Control, PCR Amplified Library의 유효성 검사에도 훌륭한 도구로 사용됩니다.

qsep-ngs-f2.jpg qsep-ngs-f3.jpg qsep-ngs-f4.jpg
Quality control of fragmented DNA. DNA is sheared into small fragments with an average size of 895 bp. Monitoring integrity of total RNA is a typical QC step for downstream experiments, such as RNA-seq. RQN (RNA Quality Number) value determines the integrity of RNA. High sensitivity result of serially diluted library sample. The average size of the DNA library is 515 bp. Library concentrations are from 0.33 ng/μl to 0.07 ng/μl.


Qsep1 Qsep100 Qsep400
System Type 1 Channel Portable 1 Channel Standard 4-Channel System
Automated sampling 1~8 samples 1~8 samples 1~96 samples

Cartridge Selection Guide

Cartridge Standard
High Resolution
Kilo Base
High Sensitivity
Sample size range 10-15000 bp 10-15000 bp 10-23000 bp 10-15000 bp 10-15000 bp N/A
L.O.D. 0.1 ng/μl* 0.1 ng/μl* 0.1 ng/μl* 0.1 ng/μl* 5pg/μl* 5pg/μl
Resolution 4-10 bp 1-4 bp 10-50 bp ≥ 50 bp ≥ 10 bp N/A
Analysis Time (per sample) 2-3min 2-3min 5-8min 1-2min 3-5min 5-10min

Routine Applications

• PCR Product Screening
• Genotyping
• Next Generation Sequencing (NGS) QC
• Restriction Fragment Length Polymorphism (RFLP)
• Oligonucleotides Analysis
• Genomic DNA Analysis
• Plasmid Purification & Vector Cloning Analysis
• RNA Analysis
• Protein Profiling (fluorescence detection)

Specific Applications

• CRISPR technology
• cfDNA (Circulating Cell-Free DNA)
• Chromatin immunoprecipitation (ChIP)
• Glycan analysis (N-Glycan)
• Immuno Assay: Ag and Ab Reaction
• Genetic profiling /disease screening
• Pathogen detection/typing (HBV, HCV, HPV, TB, AIV, STD etc.)
• Agriculture product detection (GMO, 한우판정, 식물품종 판별)
• Food Safety analysis
• SNP screening
• Microsatellite test (STR, SSR, MSI)

제품 상세정보 보러가기

Qsep1 DNA Fragment Analyzer
Qsep100 DNA Fragment Analyzer
Qsep400 DNA Fragment Analyzer
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